Corall kit

Manufactured by Lexogen

The Corall kit is a RNA library preparation solution for highly accurate and efficient 3'-end sequencing. It enables the construction of 3'-end-enriched libraries from small amounts of total RNA or poly(A)+ RNA samples.

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Lab products found in correlation

Dynabeads mrna purification kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CORALL Total RNA-Seq Library Prep Kit (18 citations) CORALL Total RNA-Seq Kit (2 citations) CORALL kit protocol (2 citations) Corall Total RNA Seq library preparation kit (2 citations)

2 protocols using corall kit

Poly-A mRNA Purification and Sequencing

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The Dynabeads mRNA purification kit (Thermo Fisher Scientific) was used to extract all RNA molecules with a poly-A tail. An amount of 1–2 µg total RNA was used as input. Eluted RNA was used as input using the Corall kit (Lexogen, Vienna, Austria) according to the manufacturer’s instructions and targeting an insert size of 350 bp.

Naboulsi R., Cieślak J., Headon D., Jouni A., Negro J.J., Andersson G, & Lindgren G. (2022). The Enrichment of Specific Hair Follicle-Associated Cell Populations in Plucked Hairs Offers an Opportunity to Study Gene Expression Underlying Hair Traits. International Journal of Molecular Sciences, 24(1), 561.

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RNA-seq of microbial communities

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RNA samples were delivered to Core Unit SysMed (Julius-Maximilian-University Würzburg) for RNA-seq. Briefly, after treating RNA samples with DNase, sufficient RNA quality was verified on a bioanalyzer (RNA chip, Agilent). Ribosomal RNA was depleted (RiboCop-META kit, Lexogen), and RNA was then reverse transcribed for cDNA library preparation using the CORALL kit (Lexogen) according to the manufacturer's instructions. After pooling cDNA library samples at equimolar amounts, quality was verified using a bioanalyzer (DNA chip, Agilent). The cDNA pools were sequenced using the NextSeq 500 system (HighOutput flow cell, 400 M, 1× 75 cycle single-end; Illumina).

Jung J., Popella L., Do P.T., Pfau P., Vogel J, & Barquist L. (2023). Design and off-target prediction for antisense oligomers targeting bacterial mRNAs with the MASON web server. RNA, 29(5), 570-583.

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