Tissue squisher

Manufactured by Zymo Research

The Tissue Squisher is a laboratory equipment used for homogenizing tissue samples. It applies mechanical force to disrupt the cellular structure and release the contents of the cells, preparing the sample for further analysis.

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Lab products found in correlation

Quick rna miniprep kit, by Zymo Research (2 mentions) 2100 bioanalyzer, by Agilent Technologies (2 mentions) Superscript 3 first strand synthesis kit, by Thermo Fisher Scientific (2 mentions) Nebnext poly a mrna magnetic isolation module, by New England Biolabs (2 mentions) Hiseq 4000, by Illumina (1 mentions) Rnaeasy kit, by Qiagen (1 mentions) Novaseq 6000 system, by Illumina (1 mentions)

2 protocols using tissue squisher

RNA-Seq Expression Profiling of Mouse Embryonic Skin

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RNA for RNA-Seq expression profiling was collected from flash-frozen dorsal skin from E15.5, 16.5, 17.5, and E18.5 mouse embryos using the Quick-RNA miniprep kit (Zymo Research) or RNAeasy kit (Qiagen) following manual homogenization using the Tissue Squisher (Zymo Research) in lysis buffer. cDNA was synthesized using 1 μg RNA using the Superscript III First Strand Synthesis Kit (Life Technologies/Thermo Fisher Scientific). RNA quality was determined using the 2100 Bioanalyzer (Agilent). One microgram RNA from each sample was used for mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (E7490S, New England Biolabs). RNA-Seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, New England Biolabs). The quality of these RNA-Seq libraries was validated using 2100 Bioanalyzer (Agilent), and sequencing was performed by NUseq Core Facility using the Illumina Hiseq4000 (1 × 50 bp) or the Advanced Genomics Core of the Skin Biology Diseases Resource–based Center at the University of Michigan using the Illumina NovaSeq 6000 system (Illumina).

Godsel L.M., Roth-Carter Q.R., Koetsier J.L., Tsoi L.C., Huffine A.L., Broussard J.A., Fitz G.N., Lloyd S.M., Kweon J., Burks H.E., Hegazy M., Amagai S., Harms P.W., Xing X., Kirma J., Johnson J.L., Urciuoli G., Doglio L.T., Swindell W.R., Awatramani R., Sprecher E., Bao X., Cohen-Barak E., Missero C., Gudjonsson J.E, & Green K.J. (2022). Translational implications of Th17-skewed inflammation due to genetic deficiency of a cadherin stress sensor. The Journal of Clinical Investigation, 132(3), e144363.

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Skin RNA-seq profiling in E18.5 mice

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-/-mice.
RNA for RNA-Seq expression profiling was collected from flash frozen dorsal skin from E18.5 mice using the Quick-RNA miniprep kit (Zymo Research) following manual homogenization using the Tissue Squisher (Zymo Research) in lysis buffer. cDNA was synthesized using 1 µg of RNA using the Superscript III First Strand Synthesis Kit (Life Technologies/Thermo Fisher). RNA quality was determined using the 2100 Bioanalyzer (Agilent). 1µg RNA from each sample was used for mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (E7490S, NEB). RNA-seq libraries were constructed using the purified mRNA samples with NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, NEB). The quality of these RNA-seq libraries were validated using 2100 Bioanalyzer (Agilent), and sequencing was performed by NUseq Core Facility using the IIlumina Hiseq4000 (1x50bp).

Godsel L.M., Roth‐Carter Q.R., Koetsier J.L., Tsoi L.C., Broussard J.A., Fitz G.N., Lloyd S., Kweon J., Huffine A.L., Burks H.E., Hegazy M., Amagai S., Harms P.W., Johnson J., Urciuoli G., Doglio L., Swindell W.R., Awatramani R., Sprecher E., Bao X., Cohen‐Barak E., Missero C., Guðjónsson J.E., & Green K.J. (2020). Th17-skewed inflammation due to genetic deficiency of a cadherin stress sensor.

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