Sk mel 28 melanoma cells

The following human cell-types were obtained from the American Type Culture Collection (ATCC; Manassas, VA), namely DMS-53 lung carcinoma cells, SK-N-MC neuroepithelioma cells, SK-Mel-28 melanoma cells, MCF-7 breast cancer cells, MRC-5 fibroblast cells, HepG2 hepatocellular carcinoma and the papillomavirus 16 transformed kidney proximal tubule HK-2 cells. All of these cell-types, except DMS-53 lung carcinoma cells, were cultured as previously described [35 (link)] in minimum essential media (MEM; Life Technologies) at 37°C. The DMS-53 lung carcinoma cell line was cultured in a similar manner as above using Roswell Park MEMorial Institute 1640 (RPMI 1640; Life Technologies). These media were supplemented with 10% (v/v) fetal calf serum (FCS; Sigma-Aldrich) and the following additives from Life Technologies: 1% (v/v) sodium pyruvate, 1% (v/v) 100× non-essential amino acids, 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamine and 0.28 μg/mL fungizone. Human umbilical vein endothelial cells (HUVECs) were kindly donated by Mr. P. Pisansarakit (Heart Research Institute, Sydney, Australia) and were cultured according to established techniques [35 (link)]. All cells were cultured in an atmosphere of 5% CO₂/95% air.