For fluorescence-guided surgery (FGS), a 15-mm transverse incision was made on the left flank of the mouse through the skin and peritoneum and kept open with a retractor. The tail of the pancreas was exposed through this incision. Anti-CEA antibody conjugated to DyLight 650 (50 μg), was injected intravenously via the tail vein in the mice in the FGS group 24 hours before surgery. A MINI MAGLITE® LED PRO flashlight (MAG INSTRUMENT, Ontario, CA, USA) coupled to an excitation filter (ET 640/30X, Chroma Technology Corporation, Bellows Falls, VT, USA) was used as the excitation light source. A Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon) coupled with an emission filter (HQ700/75M-HCAR, Chroma Technology Corporation) was used as the real-time image capturing device for FGS. BLS was performed under standard bright-field using an MVX10 microscope (Olympus). After completion of surgery, the incision was closed in one layer using 6-0 nylon surgical sutures, and the mice were allowed to recover in their cages.
Et 640 30x
Manufactured by Chroma Technology
Sourced in United States
The ET 640/30X is a specialized lab equipment product. It is designed to perform a core function within a laboratory setting. The details of its specific capabilities and applications are not included in this factual, unbiased description.
Automatically generated - may contain errors
2 protocols using et 640 30x
1
Fluorescence-Guided Pancreatic Tumor Resection
2
Fluorescence-Guided Surgery for Pancreatic Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For fluorescence-guided surgery (FGS), a 15-mm transverse incision was made on the left flank of the mouse through the skin and peritoneum which was kept open with a retractor. The tail of the pancreas was exposed through this incision. Fifty µg of anti-CA19-9 antibody, conjugated to DyLight 650, was injected via the tail vein in the mice in the FGS group 24 hours before surgery. A MINI MAGLITE LED PRO flashlight (MAG INSTRUMENT, Ontario, CA, USA) coupled to an excitation filter (ET 640/30X, Chroma Technology Corporation, Bellows Falls, VT, USA) was used as the excitation light source. A Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) coupled with an emission filter (HQ700/75M-HCAR, Chroma Technology Corporation) was used as the real-time image capturing device for FGS. BLS was performed under standard bright-field using an MVX10 microscope (Olympus, Tokyo, Japan). After completion of surgery, the incision was closed in one layer using 6-0 nylon surgical sutures, and the mice were allowed to recover in their cages.
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