Toyopearl AF-Formyl-650M resin was from Tosoh Bioscience (King of Prussia, PA, USA). Wheat germ agglutinin (WGA) was purchased from Vector Laboratories (Burlingame, CA, USA). Stainless steel columns (2×250 mm) and the Gemini C18 column were obtained from Phenomenex (Torrance, CA, USA). Ovomucoid (type III-O, free of ovoinhibitor), trypsin (TPCK treated), PNGase F, chloroform, NaOH beads (97% purity), iodomethane, 2-mercaptoethanol, dithiothreitol, iodoacetamide, trizma base, and sodium cyanoborohydride (NaCNBH3) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Water and acetonitrile (ACN) were obtained from EMD Chemicals (Darmstadt, Germany). Formic acid was from Alfa Aesar (Ward Mill, MA, USA). Trifluoroacetic acid (TFA), dimethylformamide (DMF), urea, ammonium bicarbonate, sodium chloride, magnesium chloride, and calcium chloride were all from Mallinckrodt Pharmaceuticals (St. Louis, MO, USA). C18 Zip-tips were from Agilent Technologies (Santa Clara, CA, USA). C18 Sep-Pak cartridges were from Waters (Milford, MA, USA). Empty spin columns were purchased from Harvard Apparatus (Holliston, MA, USA).
Calcium chloride
Manufactured by Mallinckrodt
Sourced in United States
Calcium chloride is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that is highly soluble in water. Calcium chloride's core function is to act as a desiccant, absorbing moisture from the surrounding environment.
Automatically generated - may contain errors
Lab products found in correlation
Magnesium chloride, by Mallinckrodt (2 mentions)
Gemini c18 column, by Phenomenex (1 mentions)
Sep pak c18 cartridge, by Waters Corporation (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Pngase f, by Merck Group (1 mentions)
Wheat germ agglutinin (wga), by Vector Laboratories (1 mentions)
Iodomethane, by Merck Group (1 mentions)
Sodium chloride (nacl), by Mallinckrodt (1 mentions)
C18 zip tips, by Agilent Technologies (1 mentions)
Naoh beads, by Merck Group (1 mentions)
Sodium cyanoborohydride nacnbh3, by Merck Group (1 mentions)
Empty spin columns, by Harvard Apparatus (1 mentions)
Ammonium bicarbonate, by Mallinckrodt (1 mentions)
Tetrachloroauric acid, by Merck Group (1 mentions)
Alpha mem, by Thermo Fisher Scientific (1 mentions)
2 protocols using calcium chloride
1
Glycoprotein Purification and Analysis
2
Thiolated Oligonucleotides for Graphite-based Biosensors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All thiolated oligonucleotides used in this study were purchased from Integrated DNA Technologies (Coralville, IA). Graphite powder (99%, 7–11 μM) was purchased from Alfa–Aesar (Heysham, Lancashire, UK). Sulfuric acid, phosphoric acid, hydrogen peroxide, trisodium citrate, sodium phosphate tribasic, sodium phosphate dibasic, boric acid, tetrachloroauric acid, dimethyl sulfoxide, apigenin, xylene, ethanol, 3,3-diaminobenzidine tetrahydrochloride, hematoxylin, and isopropanol were purchased from Sigma–Aldrich (Milwaukee, WI). Citric acid, calcium chloride, magnesium chloride, potassium permanganate, tris(hydroxymethyl)aminomethane (Tris), and hydrochloric acid were purchased from Mallinckrodt Baker (Phillipsburg, NJ). Alpha-MEM and fetal bovine serum were purchased from GIBCO (Campinas, Brazil). An MTT assay kit was purchased from Thermo Fisher Scientific (Waltham, MA). MUC1 (VU4H5) mouse mAb, anti-mouse IgG, and HRP-linked antibody were purchased from Cell Signaling Technology (Danvers, MA). Milli-Q ultrapure water (18 MΩ, Millipore, Billerica, MA) was used in all experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!