Simian RV strain SA11 (4F derivative, provided by Dr. John T. Patton, National Institutes of Health) was used to infect monkey kidney MA104 clone 1 cells (American Type Culture Collection) as described by Arnold et al., 2009 (Arnold et al., 2009 ). Cells were sub-cultured at 37°C and 5% CO2 in Medium 199 (Life Technologies) supplemented to contain 0.25 µg/ml Amphotericin B, 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% fetal bovine serum. SA11 was activated prior to infection by incubation at 37°C for 1 hour in 10 µg/ml of porcine pancreatic type IX trypsin (Sigma Aldrich).
Ma104 clone 1 cells
Manufactured by American Type Culture Collection
MA104 clone 1 cells are a cell line derived from the MA104 kidney cell line. They are commonly used in cell culture experiments and assays. The core function of MA104 clone 1 cells is to serve as a model system for various research applications.
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Lab products found in correlation
2 protocols using ma104 clone 1 cells
1
Simian Rotavirus SA11 Infection Model
2
Simian Rotavirus SA11 Infection Model
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Simian RV strain SA11 (4F derivative, provided by Dr. John T. Patton, National Institutes of Health) was used to infect monkey kidney MA104 clone 1 cells (American Type Culture Collection) as described by Arnold et al., 2009 (Arnold et al., 2009 ). Cells were sub-cultured at 37°C and 5% CO2 in Medium 199 (Life Technologies) supplemented to contain 0.25 µg/ml Amphotericin B, 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% fetal bovine serum. SA11 was activated prior to infection by incubation at 37°C for 1 hour in 10 µg/ml of porcine pancreatic type IX trypsin (Sigma Aldrich).
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