Hplc grade solvents water and acetonitrile

Manufactured by Thermo Fisher Scientific

Sourced in United States

HPLC grade solvents, including water and acetonitrile, are high-purity solvents specifically designed for use in high-performance liquid chromatography (HPLC) applications. These solvents are carefully filtered and processed to minimize impurities, ensuring consistent and reliable performance in HPLC systems.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Acetonitrile (2707 citations) HPLC-grade acetonitrile (752 citations) HPLC grade water (280 citations) HPLC grade (157 citations) HPLC grade solvents (134 citations) HPLC solvents (25 citations) HPLC water (15 citations) Water and acetonitrile (7 citations) HPLC grade water and acetonitrile (5 citations) HPLC grade water and solvents (2 citations)

4 protocols using hplc grade solvents water and acetonitrile

Quantifying Unreacted Soluble Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantification of unreacted soluble protein in the aggregation mixture was followed by periodically removing aliquots (~80 μL) from ongoing aggregation reactions followed by centrifugation at 160,000×g and injecting the supernatant (approx. 20 μL) in duplicates into an analytical reverse phase column (Agilent Zorbax-C8 2.5 × 50 mm column). Protein samples were run against a gradient of acetonitrile (0–100%) in 0.1% TFA maintained at 300 μL·min-1 flow rate in an Agilent HPLC system (1100 series). The wavelength of the UV detector was fixed at 280 nm. HPLC grade solvents (Water and Acetonitrile) used as mobile phase in the reverse phase HPLC system were obtained from Fisher Chemicals. Concentration of the unreacted soluble protein was determined from integrated A280 nm peaks using standard curves individually acquired for each protein.³⁷

Misra P., Blancas-Mejia L.M, & Ramirez-Alvarado M. (2019). Mechanistic insights into the early events in the aggregation of immunoglobulin light chains. Biochemistry, 58(29), 3155-3168.

+ Open protocol

+ Expand

Rhodium-Catalyzed Oxidation Reactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rhodium(III) trichloride hydrate was purchased from Precious Metals Online (PMO Pty Ltd.) and used as received. [Cp*Rh(μ-Cl)Cl]₂, benzo[h]quinoline, silver nitrate, sodium formate, sodium pyruvate, β-nicotinamide adenine dinucleotide reduced disodium salt (NADH), and celite were purchased from Sigma-Aldrich. MeOD-d₄, D₂O, and CDCl₃-d₁ for NMR spectroscopy and Quantofix® peroxides test sticks (1-100 mg/L range) were purchased from Sigma-Aldrich. Reduced glutathione was obtained from Alfa Aesar. Disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate, anhydrous sodium acetate, HPLC grade solvents (water and acetonitrile) with added trifluoroacetic acid with analytic grade, anhydrous DCM/pyridine and laboratory grade solvents used in syntheses were purchased from fisher scientific. A549 human lung and A2780 human ovarian cancer cell lines were purchased from the European Collection of Animal Cell Culture (ECACC, Salisbury, U.K.). Roswell Park Memorial Institute (RPMI-1640) medium, and phosphate-buffered saline (PBS) were purchased from PAA Laboratories GmbH. The total ROS/superoxide detection kit was purchased from Enzo Life Sciences. The apoptosis detection regents were purchased from Abcam.

Zhang W.Y., Bridgewater H.E., Banerjee S., Soldevila-Barreda J.J., Clarkson G.J., Shi H., Imberti C, & Sadler P.J. (2019). Ligand-Controlled Reactivity and Cytotoxicity of Cyclometalated Rhodium(III) Complexes. European journal of inorganic chemistry, 2020(11-12), 1052-1060.

+ Open protocol

+ Expand

Fabrication and Characterization of Doxorubicin-Loaded Polymer Microspheres

Check if the same lab product or an alternative is used in the 5 most similar protocols

Polybead® carboxylate microspheres of various sizes (0.5 μm, 6.0 μm and 10 μm) were purchased from PolySciences, Inc. (Warrington, PA, USA). Poly (vinyl alcohol) (PVA-fully hydrolyzed) was obtained from Sigma Aldrich (St. Louis, MO, USA). Doxorubicin hydrochloride (DOX) was procured from LC laboratories (Woburn, MA, USA). Phosphate buffered saline (PBS) pH 7.4, dimethyl sulfoxide (DMSO), and HPLC grade solvents (acetonitrile and water) were purchased from Fisher Scientific (Hampton, NH, USA). Various other assay kits and molecular biology grade reagents were obtained from several sources which have been listed along with their respective methods.

Shukla S.K., Sarode A., Kanabar D.D., Muth A., Kunda N.K., Mitragotri S, & Gupta V. (2021). Bioinspired Particle Engineering for Non-invasive Inhaled Drug Delivery to the Lungs. Materials science & engineering. C, Materials for biological applications, 128, 112324.

+ Open protocol

+ Expand

Synthesis and Characterization of MCPF-Gly and MCPA-Gly Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols

Custom synthesis of isotopically-labeled and unlabeled MCPF-Gly and MCPA-Gly standards was contracted from IsoSciences, LLC (King of Prussia, PA). Label sites for isotopically-labeled standards are indicated by asterisks in Scheme 1. The purity of the unlabeled standards is ≥97%, and the isotopic incorporation of isotopically-labeled standards is ≥99.3%. HPLC grade solvents acetonitrile and water were obtained from Fisher Scientific (Pittsburgh, PA, USA). Formic Acid (98% purity) was obtained from Sigma Chemical Company (St. Louis, MO). Pooled human urine matrix used for calibrators and quality control (QC) materials was obtained commercially from Tennessee Blood Services (Memphis, TN, USA). The urine products used for calibrators, quality control materials, and the convenience set of 96 individual urine samples were acquired from commercial sources, and the work did not meet the definition of human subjects as specified in 45 CFR 46.102 (f).

Isenberg S.L., Carter M.D., Graham L.A., Mathews T.P., Johnson D., Thomas J.D., Pirkle J.L, & Johnson R.C. (2015). Quantification of Metabolites for Assessing Human Exposure to Soapberry Toxins Hypoglycin A and Methylenecyclopropylglycine. Chemical research in toxicology, 28(9), 1753-1759.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!