The capacity of cell migration and invasion were measured with Transwell assay. The Transwell chambers with or without Matrigel (Clontech Laboratories, Inc., Mountainview, CA, USA) were put into a 24-well plate, thus forming upper and lower chambers. Cell suspension (200 µl) with density of 1×105/ml was plated in the upper chamber and the medium of the suspended cells was free of FBS. The lower chamber was added with 400 µl RPMI-1640 supplemented with 20% FBS. After incubation for 24 h at 37°C, the cells which moved to the lower surface were stained with 0.5% crystal violet for 30 min and the cells on the upper chamber were removed. The cells in the lower chamber were observed with a microscope (BX51 Olympus; Olympus Corp., Tokyo, Japan) and counted at five random fields.
Transwell chambers
Manufactured by Takara Bio
Sourced in United States
Transwell chambers are a type of cell culture insert designed for studying cell migration and invasion. They consist of an upper compartment with a porous membrane and a lower compartment. Cells are seeded in the upper compartment and their ability to migrate or invade through the porous membrane is observed and quantified.
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Lab products found in correlation
2 protocols using transwell chambers
1
Transwell Assay for Cell Migration and Invasion
2
Transwell Assay for Cell Migration
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Transwell migration chambers (8‐μm pore size; Millipore, Billerica, MA, USA) were used to measure cell migration. A total of 2 × 105 cells were suspended in the serum-free DMEM media and planted into the top chamber together. In the lower chamber, DMEM medium with 10% FBS was added. The chambers were incubated for 48 h before removal, and crystal violet was used to stain these cells from the lower chamber following 4% paraformaldehyde for 15 min. Random six fields at high magnification (Olympus, Tokyo, Japan) were chosen, and cell migration was calculated. Additionally, the invasion assays were carried out in Transwell chambers covered with matrigel (Clontech, CA, USA).
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