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P src family y416

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P-Src family Y416 is an antibody that detects phosphorylation of a conserved tyrosine residue (Y416) in the activation loop of Src family kinases. This phosphorylation event is associated with the activation of these kinases.

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Lab products found in correlation

Wnt5a b, by Cell Signaling Technology (1 mentions) P mek1 2, by Cell Signaling Technology (1 mentions) P s6k, by Cell Signaling Technology (1 mentions) Paccs79, by Cell Signaling Technology (1 mentions) Flag polyclonal, by Merck Group (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions) Total acc, by Cell Signaling Technology (1 mentions) P p90rsks380, by Cell Signaling Technology (1 mentions) P ulk1s555, by Cell Signaling Technology (1 mentions) P paxillin y118, by Cell Signaling Technology (1 mentions) Nuak1, by Cell Signaling Technology (1 mentions) Phospho fak y397, by Abcam (1 mentions) Ampk alpha, by Cell Signaling Technology (1 mentions) Mark2, by Cell Signaling Technology (1 mentions) Mark3, by Cell Signaling Technology (1 mentions) Pfak y925, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Ha tag, by Cell Signaling Technology (1 mentions) Axin2, by Cell Signaling Technology (1 mentions)

2 protocols using p src family y416

1

Signaling Pathway Antibody Analysis

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Cell Signaling Technology antibodies used: Wnt5a/b (#2530), Snail (#3879), Slug (#9585), ZEB1 (#3396), LKB1 (#3047), P-ACC S79 (#3661), Total ACC (#4190), Axin2 (#2151), MARK2 (#9118), MARK3 (#9311), AMPKalpha (#2532), P-ULK1 S555 (#5869), Nuak1 (#4458), SIK2 (#6919), GST (#2622), myc-tag (#2272), P-Src family Y416 (#2113), Src (#2109), P-Paxillin Y118 (#2541), Pathscan I for P-ERK1/2 and P-Akt S473 (#5301), Total ERK1/2 (#4695), P-S6K (#9234), HA-tag (#3724), P-MEK1/2 (#9154), P-p90RSK S380 (#11989), P-FAK Y925 (#3284). Epitomics antibodies used: Phospho-FAK Y397 (#2211-1), Phospho-FAK Y576/577 (#2183-1), Total FAK1 (#2146-1), Zyxin (#3586-1). BD Transduction Labs antibodies used: Paxillin (P13520). Sigma antibodies used: Actin (A5441), Flag polyclonal (F7425). Protein Tech antibodies used: MARK1 (21552-1-AP), MARK2 (15492-1-AP). Millipore antibodies used: ZEB2 (ABT332), MARK4 (07-699). Abcam antibodies used Twist (ab50887), IRSp53 (ab15697). CLASP2 antibody was from Santa Cruz Biotechnology (sc-98440). DIXDC1 total antibody was from R&D Systems (AF5599). Phospho-DIXDC1 S592 was developed in collaboration with Antony Wood at Cell Signaling Technologies (CST, Danvers, MA).
Goodwin J.M., Svensson R.U., Lou H.J., Winslow M.M., Turk B.E, & Shaw R.J. (2014). A novel AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Molecular cell, 55(3), 436-450.
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2

T Cell Signaling Pathway Analysis

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Antibodies used for T cell stimulation included DimerX I recombinant HLA-A2 Ig (BD Biosciences), CD3 (clone OKT3, Biolegend), and CD28 (clone CD28.2, Biolegend). Western blot antibodies included PTPN22 (clone D6D1H, Cell Signaling Technology), Lck (Cell Signaling Technology), Zap-70 (Cell Signaling Technology), α-tubulin (clone TU-02, Santa Cruz), anti-rabbit Ig (Life Technologies) and anti-mouse Ig (LI-COR). For IP-FCM, the Akt capture antibody used was clone SKB1 (Merck Millipore), total Akt was detected using clone 53G (Cell Signaling Technology), and phospho Akt S473 was detected using clone D9E (Cell Signaling). Flow cytometry antibodies included CD69 (clone FN50, BD Biosciences), Il-2 (clone MQ1-17H12, Biolegend), pSrc family Y416 (Cell Signaling Technology), Lck pY505 (Cell Signaling Technology), Zap-70 pY493 (Cell Signaling Technology), Erk 1/2 pY204 (clone 197G2, Cell Signaling Technology), NFAT (clone D43B1, Cell Signaling Technology), cFos (clone 2G2, Novus Biologicals), an anti-rabbit secondary (Invitrogen), and an anti-mouse secondary (Life Technologies).
Bray C., Wright D., Haupt S., Thomas S., Stauss H, & Zamoyska R. (2018). Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2. Frontiers in Immunology, 9, 2595.
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