Mouse tat complex elisa kit

Manufactured by Assaypro

Sourced in United States

The Mouse TAT-complex ELISA kit is a quantitative assay designed to measure the concentration of the TAT-complex in mouse samples. It employs the sandwich ELISA technique.

Automatically generated - may contain errors

Lab products found in correlation

Cytokine elisa kit, by Thermo Fisher Scientific (1 mentions) Human factor x, by Enzyme Research (1 mentions)

3 protocols using mouse tat complex elisa kit

Investigating Mycobacterium tuberculosis Pathogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gamma-irradiated H37Rv (γ-Mtb) and Mycobacterium tuberculosis (M.tb) H37Rv (27294) were obtained from ATCC (Rockville, MD). Recombinant human factor VIIa (FVIIa) was from Novo Nordisk (Gentofte, Denmark). Human factor X was purchased from Enzyme Research Laboratories (South Bend, IN). Chromogenic substrate Chromogenix S-2765 was from DiaPharma (West Chester, OH). Cytokine ELISA kits were from eBioscience (San Diego, CA). Mouse TAT-complex ELISA kit was from Assaypro (St. Charles, MO). Mouse macrophage marker antibody F4/80 (SP115) was from Novus Biologicals (Littleton, CO) and neutrophil marker Ly-6G (IA8) was from BD Pharmingen (San Diego, CA). Preparation and characterization of monospecific polyclonal antibodies against human TF were described earlier [36] (link). Rabbit polyclonal anti-mouse TF antibody was kindly provided by Lars C. Petersen, Novo Nordisk, Denmark.

Kothari H., Keshava S., Vatsyayan R., Mackman N., Rao L.V, & Pendurthi U.R. (2014). Role of Tissue Factor in Mycobacterium tuberculosis-Induced Inflammation and Disease Pathogenesis. PLoS ONE, 9(12), e114141.

+ Open protocol

+ Expand

Mouse Plasma TAT and ECP Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse blood was drawn into syringes containing 10% 0.1 M citrate and immediately put on ice. Plasma was obtained by 10-min centrifugation (3,000 g) and stored at −80°C until analysis. The following ELISA kits were used: Mouse TAT Complex ELISA kit (EMT1020-1; Assaypro) and Human ECP ELISA kit (SK00128-01; Aviscera Bioscience). ELISAs were performed according to the manufacturer’s specifications.

Uderhardt S., Ackermann J.A., Fillep T., Hammond V.J., Willeit J., Santer P., Mayr M., Biburger M., Miller M., Zellner K.R., Stark K., Zarbock A., Rossaint J., Schubert I., Mielenz D., Dietel B., Raaz-Schrauder D., Ay C., Gremmel T., Thaler J., Heim C., Herrmann M., Collins P.W., Schabbauer G., Mackman N., Voehringer D., Nadler J.L., Lee J.J., Massberg S., Rauh M., Kiechl S., Schett G., O’Donnell V.B, & Krönke G. (2017). Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease. The Journal of Experimental Medicine, 214(7), 2121-2138.

+ Open protocol

+ Expand

Mouse TAT Complex ELISA Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse TAT complex levels were measured with the mouse TAT Complex ELISA Kit (Assaypro, St Charles, MO, USA).

SUNDARAM J., KESHAVA S., GOPALAKRISHNAN R., ESMON C.T., PENDURTHI U.R, & RAO L.V. (2014). Factor VIIa binding to endothelial cell protein C receptor protects vascular barrier integrity in vivo. Journal of thrombosis and haemostasis : JTH, 12(5), 690-700.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!