BTX-A (Hengli, Lanzhou Institute of Biological Products, China) was dissolved in 5 mL physiological saline to achieve a final concentration of 20 U/mL. Perfluoropropane microbubbles (SunLipo NanoTech, China) were prepared following the manufacturer’s instructions. The MB and BTX-A mixture was obtained by dissolving freeze-dried BTX-A powder in 5 mL of MB solution. This MB and BTX-A mixture was selected for subsequent experiments. The morphology of the microbubbles was examined using a transmission electron microscope (Ht-7700, Hitachi). The size distribution and zeta potential of the microbubbles were measured using a particle size and zeta potential analyzer (Malvern Instruments).
Particle size and zeta potential analyzer
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Particle size and zeta potential analyzer is a lab equipment used to measure the size distribution and surface charge (zeta potential) of particles in a sample. It provides accurate and reliable data on the physical characteristics of particles in various liquid or powder-based materials.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using particle size and zeta potential analyzer
1
Botulinum Toxin and Microbubble Formulation
2
Nanoparticle Characterization and CMC Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particle size and zeta potential are measured using a dynamic light scattering-based nanoparticle size analyzer (Malvern particle size and zeta potential analyzer, Malvern, Britain). Dilution of the prepared nanoparticles to a concentration of 0.2 mg/mL was carried out with ultrapure water. Later on, we carried out appropriate analysis, namely determination of the intensity distribution, polydispersity index (PDI) and zeta potential. Transmission electron microscopy (TEM, JEM-1400, JEOL, Tokyo-Japan) was utilized to examine the size and surface morphology of the nanoparticles. Aliquot of diluted nanoparticles (0.1 mg/mL) was deposited onto a copper grid, followed by infrared drying. Subsequently, a phosphotungstic acid-negative staining solution was applied for 1 minute, while excess stain was removed. After air- and static drying, the copper grid was examined under the TEM to observe and document the morphological features of the nanoparticles. The determination of critical micelle concentration (CMC) was described in the Supplementary Material 1
3
Characterization of MON@PG Nanocomposite
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The elemental mapping and surface morphology of the MON@PG were investigated via a transmission electron microscope (TEM, S-450, Hitachi Limited, Japan). Particle Size And Zeta Potential Analyzer (Malvern Panalytical, United Kingdom) was applied to measure the average size of the nanocomposite. The optical properties of MON@PG was analyzed by UV–Vis spectroscopy (UV-2600, Shimadzu Vietnam Co., Ltd., Japan). Elemental composition of MON was investigated by energy dispersive spectroscopy (EDS, Oxford Instruments, United Kingdom).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!