All tissue donors or a proxy provided written or verbal consent to donate postmortem brains for use in biomedical research in accordance with the standards of each institution. Fresh-frozen autopsied brain tissue was procured from several brain biorepositories in the United States and Europe (SI Appendix, Tables S1 and S2 include available demographic information of patient donors). Samples were chosen based on available cases and each cohort was age- and sex-matched as a group. Frozen tissues were thawed and weighed to determine the mass in grams. Tissue was mechanically homogenized in nine volumes of cold Dulbecco’s phosphate-buffered saline (DPBS) containing Halt Protease Inhibitor Mixture (1×; Thermo Fisher Scientific) using a handheld probe-tip homogenizer (OMNI International). The homogenate was clarified by centrifugation at 5,000g for 5 min at 4 °C, and the supernatants were collected and stored at −80 °C.
Handheld probe tip homogenizer
Manufactured by Omni International
The Handheld probe-tip homogenizer is a laboratory equipment designed for rapid and efficient homogenization of samples. It features a compact, handheld design and a probe-tip that can be submerged directly into the sample to perform the homogenization process.
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Lab products found in correlation
2 protocols using handheld probe tip homogenizer
1
Postmortem Brain Tissue Homogenization
2
Procuring and Preserving Autopsied Brain Tissue
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All tissue donors provided written or verbal consent to donate autopsied brains for use in biomedical research in accordance with the standards of each institution. Fresh-frozen autopsied brain tissue was procured from the brain biorepositories at UCSF and the Guam Brain Specimen repository of the Micronesian Health Study, directed by Dr. Daniel Perl and funded by the National Institute on Aging for over 20 y. It is currently housed at the Uniformed Services University of the Health Sciences in Bethesda, MD. SI Appendix, Tables S1 and S2 include available demographic and clinicopathological information of patient donors. Frozen brain tissues were thawed and weighed to determine the mass in grams. Tissue was mechanically homogenized in nine volumes of cold Dulbecco's phosphate-buffered saline (DPBS) containing Halt Protease Inhibitor Cocktail (1×, Thermo Fisher Scientific), using a handheld probe-tip homogenizer (OMNI International). The homogenate was clarified by centrifugation at 5,000 × g for 5 min at 4 °C, and supernatants were collected and stored at −80 °C.
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