Td 94149

The results from this study used samples from our previously reported work (Wahlang et al., 2017 (link)). Briefly, C57BL/6 male mice at 8 weeks of age were purchased from Taconic (Hudson, NY, USA) and were allowed to acclimate for a period of 1 week. Mice were divided into 4 study groups, including CD+vehicle, CD+PCB 126, MCD+vehicle, and MCD+PCB 126. Animals were fed either the amino acid control diet (CD; TD.94149; Envigo, Madison, WI, USA) or the methionine-choline deficient diet (MCD; TD.90262, Envigo) for a period of 4 weeks. Then the mice were treated by intragastric gavage with 1.53 µmol kg⁻¹ of PCB 126 (AccuStandard, CT, USA) in corn oil or vehicle alone. Animals were euthanized (ketamine/xylazine, 100/20 mg kg⁻¹ body weight, i.p.) at week 14. Tissues were harvested and stored at −80 °C prior to analysis. The animal protocol was approved by the University of Kentucky Institutional Animal Care and Use Committee.

C57BL/6J male mice were housed in ventilated cages with corn cob bedding and maintained on a 14:10-h light/dark cycle with ad libitum access to standard nonpurified diet (Envigo #2918). At 10 weeks of age, mice were randomly divided into two groups. One group was fed an MCD diet (Envigo #TD.90262), and a control group was fed a nutrient-matched control diet (CTR) (Envigo #TD.94149) containing sufficient methionine and choline. Mice were fed the diets ad libitum for 3 weeks. During the final week of the study, each diet group was divided into two subgroups (n=8 per group) that received intraperitoneal (IP) injections every other day (four total) of the following solutions: vehicle [1% dimethylsulfoxide (DMSO) in saline: Veh] or alloxazine (Alx) at a dose of 0.5 mg/kg body weight dissolved in vehicle. All study protocols were approved by the Institutional Animal Care and Use Committee of the University of Florida and were in accordance with the Guide for the Care and Use of Laboratory Animals (NIH publication 86–23 revised 1985).