Chx c7698

Manufactured by Merck Group

Sourced in United States, China

The CHX (C7698) is a laboratory equipment product from Merck Group. It is designed for general laboratory use, but a detailed factual description cannot be provided while maintaining an unbiased and concise approach. The core function of this product is not available for an objective presentation at this time.

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Lab products found in correlation

Goat polyclonal anti tia 1 sc 1751, by Santa Cruz Biotechnology (1 mentions) Ab220162, by Abcam (1 mentions) Ab207612, by Abcam (1 mentions) Ab220161, by Abcam (1 mentions) Ab220163, by Abcam (1 mentions) Ab92821, by Abcam (1 mentions) Ab208577, by Abcam (1 mentions) Ab220160, by Abcam (1 mentions) Mg132 m7449, by Merck Group (1 mentions) Gbm cell lines, by American Type Culture Collection (1 mentions) Z vad fmk hy 16658, by MedChemExpress (1 mentions) Sharpin 14626 1 ap, by Proteintech (1 mentions)

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C7698 (22 citations)

4 protocols using chx c7698

Immunostaining of Stress Granule Proteins

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Mouse monoclonal anti-Flag (F1804), rabbit polyclonal anti-GFP (G1544), sodium arsenite (ARS, S7400), and CHX (C7698) were purchased from Sigma-Aldrich (USA). Goat polyclonal anti-TIA-1 (sc-1751) and mouse monoclonal anti-PABP (sc-32318) antibodies were purchased from Santa Cruz (USA). Mouse monoclonal anti-G3BP (ab56574) antibody was purchased from Abcam (USA). Rabbit polyclonal anti-eIF3A (sc-2538) antibody was purchased from Cell Signaling Technology (USA). Horseradish peroxidase-conjugated donkey anti-goat, goat anti-rabbit, and anti-mouse secondary antibodies were purchased from Jackson Immuno Research (USA). Alexa Fluor 488-labeled donkey anti-goat IgG (A11055), Alexa Fluor 594-labeled goat anti-rabbit (A11012), and goat anti-mouse (A-11005) were purchased from Thermo Fisher Scientific (USA).

Sun Y., Zhang P., Zheng H., Dong L., Tan L., Song C., Qiu X., Liao Y., Meng C., Yu S, & Ding C. (2018). Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues. Journal of Veterinary Science, 19(1), 3-12.

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Comprehensive Analysis of m6A Regulators

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Ferrostatin-1 (S7243), necrostatin-1 (S8037), Z-VAD-FMK (S7023), liproxstatin-1 (S7699), sorafenib (S7397), erastin (S7242), RSL3 (S8155), MG-132 (S2619) were bought from Selleck Chemicals. CHX (C7698) and Act-D (129935) were purchased from Sigma-Aldrich. Anti-N⁶-methyladenosine antibody (ab208577), anti-METTL3 antibody (ab195352), anti-METTL4 (ab107540), anti-METTL14 antibody (ab220030), anti-FTO antibody (ab92821), anti-ALKBH5 antibody (ab195377), anti-YTHDF1 antibody (ab220162), anti-YTHDF2 antibody (ab220163), anti-YTHDF3 antibody (ab220161), anti-YTHDC2 antibody (ab220160), anti-HNRNPA2B1 antibody (ab31645), anti-ATG3 antibody (ab108282), anti-ATG4A antibody (ab223374), anti-ATG5 antibody (ab108327), anti-BECN1 antibody (ab207612), anti-ATG7 antibody (ab133528), anti-ATG9A antibody (ab108338), anti-ATG12 antibody (ab155589), anti-ATG16L1 antibody (ab187671), anti-LC3-I/II antibody (ab128025), anti-P62 antibody (ab109012), anti-NCOA4 antibody (ab86707), anti-FTH1 antibody (ab65080), and anti-beta actin (ab6276) antibody were purchased from Abcam Technology. Anti-WTAP antibody (sc-374280) was bought from Santa Cruz Biotechnology. Anti-Mouse IgG (G-21040) and anti-Rabbit IgG (G-21234) were bought from Thermo Fisher Scientific.

Shen M., Li Y., Wang Y., Shao J., Zhang F., Yin G., Chen A., Zhang Z, & Zheng S. (2021). N6-methyladenosine modification regulates ferroptosis through autophagy signaling pathway in hepatic stellate cells. Redox Biology, 47, 102151.

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Glioblastoma Cell Line Culture Protocol

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All GBM cell lines and normal SVGP12 cells were originally purchased from ATCC (American Type Culture Collection, Rockville, MD, USA). Cells were cultured in DMEM/DME/F-12 1:1 (Dulbecco's modified Eagle's medium/Ham's nutrient mixture F-12) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (P/S). All cell lines were incubated in a humidified atmosphere at 37 °C and 5% CO₂. The growth media, reagents, and supplements were obtained from Gibco. HA (H3663) and Flag (SAB4200071) antibodies, MG-132 (M7449), and CHX (C7698) were acquired from Sigma (Shanghai, China); PHF19 (77271 S), β-catenin (ab32572), MMP7 (ab38996) antibodies and the Cell Cycle Antibody Sampler Kit (no. 9932), the Cyclin Antibody Sampler Kit (no. 9869), and the Epithelial–Mesenchymal Transition Antibody Sampler Kit (no. 9782) were purchased from Cell Signaling Technology (Shanghai, China). SIAH1 (ab2237), C-myc (ab32), and Axin2 (ab109307) antibodies were obtained from Abcam (Shanghai, China). All antibodies were diluted according to the instructions.

Deng Q., Hou J., Feng L., Lv A., Ke X., Liang H., Wang F., Zhang K., Chen K, & Cui H. (2018). PHF19 promotes the proliferation, migration, and chemosensitivity of glioblastoma to doxorubicin through modulation of the SIAH1/β–catenin axis. Cell Death & Disease, 9(11), 1049.

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Antibodies and Reagents for Cell Signaling

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The following antibodies and reagents were used in this study: PP6 (15852-1-AP), PPP6R1 (17819-1-AP), PPP6R3 (16944-1-AP) and Sharpin (14626-1-AP) were purchased from Proteintech Group. PPP6R2 (sc-376238) and FADD (sc-6036) were purchased from Santa Cruz. Caspase-8 (4927), Cl-Caspase-8 (8592), Caspase-3 (9662), Cl-Caspase-3 (9661), c-FLIP (56343), PARP (9532), IKKα (2682), IKKβ (2678), p-IKKα/β (2697), RIPK1 (3493), p-S166-RIPK1 (31122), p-T231/S232-RIPK3 (57220), TBK1 (3504), p-S172-TBK1 (5483) and A20 (5630) were purchased from Cell Signaling Technology. MLKL (ab183770), p-S345-MLKL (ab196436), and FADD (ab124812) were purchased from Abcam. TNFR1 (AF-425-PB) and pan-cIAP (MAB3400) were purchased from R&D Systems. β-actin (I10813) was purchased from TransGen. Human recombinant TNFα (C008) was purchased from Novoprotein. 5Z-7 (O9890) and CHX (C7698) were purchased from Sigma. PS341 and (HY-10227) and zVAD.fmk (HY-16658) were purchased from MedChemExpress. M1 and K63 ubiquitin antibodys were gifts from Dr. Vishva Dixit of Genentech [65 (link)].

Wu G., Li D., Liang W., Sun W., Xie X., Tong Y., Shan B., Zhang M., Lu X., Yuan J, & Li Y. (2022). PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death. Cell Death & Disease, 13(9), 773.

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