The study of the degree of crystallinity and polymorphs of the lipid matrix of the obtained lipid nanoparticles was carried out based on the results of diffraction analysis obtained with the use of the D8 Advance powder diffractometer with a Johansson monochromator (Bruker). For each tested sample, the procedure was performed once, using the high angle range (2Θ = 6.0–60.0°) and obtaining results in the form of diffractograms.
The study of polymorphic variants of the lipid matrix of the obtained lipid nanoparticles was also carried out based on the results of the DSC thermal analysis obtained with the use of the DSC 8500 differential scanning calorimeter (Perkin Elmer, USA). The measuring principle was to gradually heat the sample from 25°C to 90°C in nitrogen flow (20 mL/min) at a scanning rate of 5°C per minute, keep the sample at 90°C for 1 minute, and then cool it to 25°C with similar parameters. For each tested sample, the procedure was performed once, obtaining results in the form of DSC curves (a graph of the relationship between the measured difference of heat fluxes and temperature) and the determined melting point and reaction enthalpy.
The morphology of lipid nanoparticles was determined using a scanning electron microscope (SEM).
The study of polymorphic variants of the lipid matrix of the obtained lipid nanoparticles was also carried out based on the results of the DSC thermal analysis obtained with the use of the DSC 8500 differential scanning calorimeter (Perkin Elmer, USA). The measuring principle was to gradually heat the sample from 25°C to 90°C in nitrogen flow (20 mL/min) at a scanning rate of 5°C per minute, keep the sample at 90°C for 1 minute, and then cool it to 25°C with similar parameters. For each tested sample, the procedure was performed once, obtaining results in the form of DSC curves (a graph of the relationship between the measured difference of heat fluxes and temperature) and the determined melting point and reaction enthalpy.
The morphology of lipid nanoparticles was determined using a scanning electron microscope (SEM).