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10 protocols using model jem 2100

1

Glycofullerene Characterization by TEM

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Transmission electron microscopy (TEM) was performed by using a JEOL microscope (Model JEM-2100) (JEOL Ltd., Tokyo, Japan) operated at 200 keV to analyze the sizes and dispersion of the synthetic glycofullerenes. A drop of the glycofullerenes solution (~1 μL) was dropped on a carbon-coated 200-mesh copper grid. The grid was left to dry at room temperature for hours. Before the TEM analysis, the grid was then further dried under vacuum overnight.
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2

TEM Analysis of GO and GO-HN2 Platelets

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TEM micrographs of GO and GO-HN2 platelets were obtained by JEOL TEM (model JEM-2100, Japan), operated at 200 kV using Holey carbon film on 300 mesh nickel grids.
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3

Structural and Morphological Characterization

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The crystal structure of the samples was determined via X-ray powder diffraction (XRD, Philips PC-APD) with Cu Kα radiation at 25 °C between 10 and 80°. The morphology of the samples was characterized via scanning electron microscopy (SEM, SU8000, Hitachi, Japan). Transmission electron microscopy (TEM, JEOL, Model JEM-2100) was used to investigate the lattice structure and surface feature of samples. TGA was conducted on a TGA/DSC 1 thermogravimetric analyzer (Mettler Toledo) in air from room temperature to 450 °C. Fourier transform infrared spectroscopy (FTIR) was performed on Perkin Elmer's Spectrum Two L160000A.
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4

Morphological Analysis of Solid Dispersions

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The morphology of SOLU, GSE, GSE-SOLU solid dispersions was also analysed by transmission electron microscopy (JEOL Model-JEM2100). The samples were diluted with deionized water and a drop of all the samples was individually placed on the grid and dried prior to analysis. Then, the samples were subjected to TEM analysis.
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5

Comprehensive Characterization of Carbon Dots

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Photoluminescence (PL) spectrum, UV-vis absorption spectrum, QY as well as PL lifetime were recorded using fluorescence spectrometer (FLS980) from Techcomp (China). Transmission electron microscopy (TEM), Model JEM-2100 and JEM-2100F-JEOL, was used to characterize the surface morphology of the prepared CDs. Subsequently, high-resolution TEM (HRTEM) images of the as-prepared CDs were obtained with a Model JEM-2100 transmission electron microscope. The solution was drop-casted onto mica substrates for Atomic force microscopy (AFM) investigations by using the Bruker Scan-Dimension-Icon System. X-ray photoelectron spectroscopy (XPS) were collected on a Thermo Scientific ESCALAB 250 Multi-technique surface analysis. A Bruker Vertex 70 spectrometer were used to measure the Fourier transform infrared (FTIR) spectrum spectra. X-ray diffraction spectroscopic (XRD) patterns were carried out by an X-ray diffraction using Cu-Kα radiation (XRD, PANalytical X'Pert Pro MPD). The photographs of CDs were taken with a mobile phone under UV light excited at 365 nm.
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6

Characterizing Glycofullerene Nanostructures by TEM

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Transmission electron microscopy (TEM) was performed using a JEOL microscope (Model JEM-2100) operated at 200 keV, to characterize the sizes and dispersion of the glycofullerenes. A drop of fullerenol solution (~1 μL) was placed on a carbon-coated 200-mesh copper grid. The grid was allowed to dry at room temperature for several hours and further dried under vacuum conditions overnight, prior to TEM analysis.
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7

Multimodal Characterization of Nanomaterials

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Raman measurements were recorded at a wavelength of 514.5 nm using confocal Raman microscope by Renishaw (In Via Raman). TEM analyses of the samples were done using a JEOL JEM-2100 model. The fluorescent study was carried out with an RF-5301 PC, Shimadzu fluorescence spectrometer. Functional groups were qualitatively identified by a Fourier transform infrared spectrometer (Thermo Nicolet 370 spectrophotometer). Atomic force microscope (Witec Alpha 300RA) was used for the height profile analysis. The UV/Vis spectrometer (Ocean optics JAZ series) was used for acquiring absorption spectra. The CHNS analysis was carried out using Elementar Vario EL111 elemental analyzer.
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8

Characterization of M-TiO2@rGO Composites

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The compositions of M-TiO2@rGO and M-TiO2 were characterized by X-ray diffractometer (XRD) with copper Kα radiation (λ = 1.5418 Å) and Raman spectroscopy (RENISHAW, REF 2000, 514.5 nm laser). The surface functional groups of M-TiO2@rGO were checked by XPS (Axis Ultra DLD, Kratos Analytical). The morphologies of the M-TiO2@rGO, M-TiO2, and Ti2CTx were analyzed by a field-emission scanning electron microscope (SEM, JEOL, JSM7500F) and a transmission electron microscope (TEM, JEOL, JEM-2100 model). Nitrogen adsorption–desorption isotherms were tested on a Quantachrome NOVA 2000e sorption analyzer at 77 K with liquid nitrogen. The rGO content of samples was tested by thermogravimetry (TA-Instruments-Wutersllc, TGA 500). The oxygen vacancies were checked through the electron paramagnetic resonance (EPR) spectra, which were recorded on a Bruker EPR ELEXSYS 500 spectrometer.
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9

Comprehensive Materials Characterization

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Raman measurements were performed at an excitation wavelength of 514 nm using Horiba LAB AM-HR spectrometer. The compositional analyses of the samples were carried out using X-ray photoelectron spectroscopy (XPS Omicron ESCA probe with monochromatic (Al) X-radiation. TEM analyses of the samples were done using a JEOL JEM-2100 model. The fluorescent study was carried out with an RF-5301 PC, Shimadzu fluorescence spectrometer. Functional groups were qualitatively identified by a Fourier transform infrared spectrometer (Thermo Nicolet 370 spectrophotometer). The UV/Vis spectrometer (Ocean optics JAZ series) was used for acquiring absorption spectra. The CHNS analysis was carried out on the instrument Elemental Vario EL111 elemental analyzer.
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10

Membrane Characterization via Advanced Microscopy

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Field emission scanning electron microscopy (FESEM) with energy dispersive X-ray spectroscopy (FESEM-EDX; Quanta FEG 250; Japan) was applied to describe the morphologies of the introduced membranes. Creating a high-resolution image with a better conductive surface, a 10 nm gold film was applied to the small-size membranes before investigation using FESEM. Nanofibers of the composite membranes were analyzed with transmission electron microscopy (TEM), using JEOL’s JEM-2100 model. FT-IR (4100, JASCO, Tokyo, Japan) was used to evaluate the chemical bonding of blended membranes. Moreover, the water contact angle was used to test membrane wettability using the DPRO image standard (Phoenix, SEO, New York, NY, USA).
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