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Anti mouse and anti rabbit igg horseradish peroxidase conjugated secondary antibodies

Manufactured by Cell Signaling Technology
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Anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies are laboratory reagents used to detect and quantify primary antibodies in various immunoassays. They consist of antibodies specific to mouse or rabbit immunoglobulin G (IgG) that are conjugated to the enzyme horseradish peroxidase.

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Lab products found in correlation

Rabbit anti akt, by Cell Signaling Technology (3 mentions) Sds page sample buffer, by Bio-Rad (3 mentions) P0191, by Teknova (3 mentions) Rabbit anti ser505 phospho dakt, by Cell Signaling Technology (3 mentions) Hybridoma bank ab 579794, by Developmental Studies Hybridoma Bank (3 mentions) Pvdf membrane, by Bio-Rad (3 mentions) Polyacrylamide gel, by Bio-Rad (3 mentions)

Close spelling variants of this product

Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit IgG secondary antibodies Horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies Horseradish peroxidase-conjugated secondary antibodies (anti-mouse/rabbit IgG) Anti-mouse and anti-rabbit horseradish peroxidase-conjugated IgG antibodies Horseradish peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies Horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies Anti-mouse and anti-rabbit peroxidase conjugated secondary antibodies Anti-mouse and anti-rabbit secondary antibodies Horseradish peroxidase-conjugated IgG antibodies Anti-mouse and anti-rabbit IgG antibodies

3 protocols using anti mouse and anti rabbit igg horseradish peroxidase conjugated secondary antibodies

1

Quantifying Drosophila AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Robins W.P., Berkey C.D., Mekalanos J.J, & Watnick P.I. (2018). The Drosophila immune deficiency pathway modulates enteroendocrine function and host metabolism. Cell metabolism, 28(3), 449-462.e5.
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2

Quantifying Drosophila AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Wong A.C., Vanhove A.S., Hang S., Purdy A.E., Pearson K.K., Asara J.M., Ali A., Morris JG J.r, & Watnick P.I. (2017). Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. Nature microbiology, 3(2), 243-252.
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3

Quantifying Drosophila AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Wong A.C., Vanhove A.S., Hang S., Purdy A.E., Pearson K.K., Asara J.M., Ali A., Morris JG J.r, & Watnick P.I. (2017). Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. Nature microbiology, 3(2), 243-252.
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