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Murine or human cytokines

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom

Murine or human cytokines are proteins secreted by various cells in the body that play a crucial role in cell signaling and immune response. These cytokines can be used in a variety of laboratory applications, such as cell culture and immunoassays.

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3 protocols using murine or human cytokines

1

Diverse Reagent Sources for Experimental Protocols

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Common reagents were from Sigma-Aldrich-Merck (St Louis, MO, USA) or Roche (Darmstadt, DE). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Abcam (Cambridge, UK) or Cell Signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, ES).
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2

Cytokine and Antibody Protocol

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Common reagents were from Sigma-Aldrich-Merck (St Louis, MO, USA) or Roche (Darmstadt, Germany). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Abcam (Cambridge, UK) or Cell signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA). Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, Spain).
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3

Immunoblotting and Cell Culture Protocols

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Common reagents including z-VAD.FMK were from Sigma-Aldrich (St Louis, MO, USA) or Roche (Darmstadt, Germany). Murine or human cytokines were obtained from PeproTech (London, UK). Antibodies were from Ambion (Austin, TX, USA), Santa Cruz Biotech (Santa Cruz, CA, USA) or Cell Signaling (Danvers, MA, USA). Reagents for electrophoresis were from Bio-Rad (Hercules, CA, USA) and Sigma-Aldrich. Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen and Gibco (Life Technologies/Thermo Fisher, Madrid, Spain).
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