Pcdna3 yset2

Manufactured by Addgene

pCDNA3-ySet2 is a yeast expression plasmid. It contains a yeast-optimized SET2 gene, which encodes a histone methyltransferase involved in transcriptional regulation.

Automatically generated - may contain errors

Lab products found in correlation

Setd2 halotag human orf in pfn21a, by Promega (2 mentions) Scramble sirna, by Horizon Discovery (1 mentions) Oligonucleotide, by Integrated DNA Technologies (1 mentions) Sirnas, by Horizon Discovery (1 mentions) Setd2 gfp, by Addgene (1 mentions) Plenti puro ha ubiquitin, by Addgene (1 mentions) Ptagrfp c1 fibrillarin, by Addgene (1 mentions)

Spelling variants of this product

PcDNA3.1 (119 citations)

4 protocols using pcdna3 yset2

Plasmid Constructs of hnRNP L and SETD2

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hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase, NEB) using full-length hnRNP L and SETD2, respectively, as a template and individual fragments were cloned. All constructs generated were confirmed by sequencing. pCDNA3-ySet2 were procured from Addgene. siRNA for setd2 and hnrnpl as well as scramble-siRNA sequence were procured from Dharmacon.

Bhattacharya S., Levy M.J., Zhang N., Li H., Florens L., Washburn M.P, & Workman J.L. (2021). The methyltransferase SETD2 couples transcription and splicing by engaging mRNA processing factors through its SHI domain. Nature Communications, 12, 1443.

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Dissecting hnRNP L, LL, and SETD2 Regulation

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hnRNP L, hnRNP LL, and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L, LL, and SETD2 were constructed by PCR (Phusion polymerase, NEB) using a full-length version of the constructs, respectively as a template, and individual fragments were cloned. All constructs generated were confirmed by sequencing. pCDNA3-ySet2 were procured from Addgene. siRNA for setd2, hnrnpll, and hnrnpl as well as scramble siRNA sequences were procured from Dharmacon.

Bhattacharya S., Wang S., Reddy D., Shen S., Zhang Y., Zhang N., Li H., Washburn M.P., Florens L., Shi Y., Workman J.L, & Li F. (2021). Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing. Nature Communications, 12, 6452.

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SETD2 Protein Characterization

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SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase, NEB) using full-length SETD2 as a template and individual fragments were cloned. All constructs generated were confirmed by sequencing. SETD2-GFP, mRFP-Ub, pLenti puro HA-Ubiquitin, pTagRFP-C1-Fibrillarin and pCDNA3-ySet2 were procured from Addgene. siRNAs were purchased from Dharmacon and oligonucleotides were procured from Integrated DNA Technologies (IDT).

Bhattacharya S, & Workman J.L. (2020). Regulation of SETD2 stability is important for the fidelity of H3K36me3 deposition. Epigenetics & Chromatin, 13, 40.

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SETD2 Deletion Mutant Generation

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SETD2-HaloTag human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase, NEB) using full-length SETD2 as a template, and individual fragments were cloned. All constructs generated were confirmed by sequencing. pCDNA3-ySet2 was procured from Addgene.

Bhattacharya S., Lange J.J., Levy M., Florens L., Washburn M.P, & Workman J.L. (2021). The disordered regions of the methyltransferase SETD2 govern its function by regulating its proteolysis and phase separation. The Journal of Biological Chemistry, 297(3), 101075.

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