Stx expression was determined for filtered culture supernatants with or without mitomycin C induction, by using RIDASCREEN Kit Verotoxin enzyme immunoassay (R-Biopharm Latin America) and then semi-quantified, as previously described [20 (link)]. To measure Vero cell cytotoxicity, the strains were cultured overnight at 37°C in 5 ml of LB broth and the culture was centrifuged at 200 rpm. The supernatant was filtered (0.22 μm filters) and subsequently assayed for cytotoxicity on Vero cells, as previously described [20 (link)].
Ridascreen kit verotoxin enzyme immunoassay
Manufactured by R-Biopharm
The RIDASCREEN Kit Verotoxin enzyme immunoassay is a laboratory equipment product for the detection of Verotoxin (Shiga toxin) in food and environmental samples. It is used for the qualitative determination of Verotoxin.
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2 protocols using ridascreen kit verotoxin enzyme immunoassay
1
Stx Expression and Vero Cell Cytotoxicity
2
Stx Expression Quantification in Supernatants
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The expression of Stx was determined in filtered culture supernatants, with the addition or absence of mitomycin C, by using RIDASCREEN Kit Verotoxin enzyme immunoassay (R-Biopharm Latin America). Its expression was semi-quantified according to Beutin et. al [34 (link)].
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