α mouse alexa647

α-Cx45-CT(MAB3101), α-mouse-HRP (H+L; 12-439), α-pS (05-1000), and α-Nedd4 (07-049) were purchased from EMD Millipore; α-Cx43 (C6219), α-β-catenin (C2206), and α-β-tubulin (T5201) were purchased from Sigma-Aldrich; α-E-cadherin-Alexa488 (#3195), α-HA-647 (#3444), α-Myc-555 (#3756), α-rabbit-Alexa555 (#4413), α-mouse-Alexa647 (#4410), Normal Rabbit IgG (#2729), and α-pY-1000 Multi-Mab (#8954) were purchased from Cell Signaling; α-mouse-Alexa488 (A-11001), Texas Red Dextran (10,000 Da; D1863), Lucifer yellow CH (L453), and Streptavidin-Alexa647 (S21374) were purchased from Life Technologies; α-rabbit-HRP (H+L; #20320) was purchased from Alpha Diagnostics; Neurobiotin (SP-1120) was purchased from Vector Labs; α-rabbit-HRP (Light chain; #211-032-171) was purchased from Jackson Immuno Research; α-Cx45-CL (ACC-207) was purchased from Alomone; Veriblot-HRP (ab131366) was purchased from Abcam; and DAPI (#02157574) was purchased from MP Biomedicals.

Cx43 pY247 and pY265 antibodies were generous gifts from Dr. Paul Lampe (Fred Hutchinson Cancer Research Center). The Cx43 pY313 antibody was produced in rabbit from a peptide designed and chemically synthesized (LifeTein). Additional antibodies used in this study include α-Src (#2123S), α-phospho-Src (Y416) (#6943S), α-rabbit-Alexa488 (#4413), α-mouse-Alexa647 (#4410), Normal Rabbit IgG (#2729), and α-pTyr-1000 Multi-Mab (#8954) purchased from Cell Signaling; α-mouse-HRP (H+L; #12–439) and α-Drebrin (#AB10140) purchased from EMD Millipore; α-Cx43 (#C6219) purchased from Sigma-Aldrich; Texas Red Dextran (3 kDa; #D1863) and Lucifer yellow CH (#L453) purchased from Life Technologies; α-rabbit-HRP (H+L; #20320) purchased from Alpha Diagnostics; α-Cx43 (ab87645 and ab15189) and α-goat-Alexa555 (ab150130), α-rabbit-Alexa647 (ab150079) purchased from Abcam; and DAPI (#157574) purchased from MP Biomedicals.