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Human tenocytes

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Human tenocytes are primary cells derived from human tendon tissue. They are used for in vitro cell culture research and experiments.

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Lab products found in correlation

Tenocyte medium, by ZenBio (2 mentions) Bsa conjugated palmitate, by Merck Group (1 mentions) Sodium palmitate, by Merck Group (1 mentions) 5 aminoimidazole 4 carboxamide ribonucleotide aicar, by Merck Group (1 mentions) 3 methyladenine 3 ma, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Scramble control, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Lipofectamin 2000, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Il 1β, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions)

6 protocols using human tenocytes

1

Effect of Substance P on Tenocytes

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The Human tenocytes were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) penicillin-streptomycin. Human tenocytes were purchased from Zen-Bio (NC, USA). The cells were divided into three groups based on the duration of SP exposure: Control (no SP treatment), Once (10−5 M SP once on day 0), and Daily (10−5 M SP daily for 10 days). For the Control and Once groups, the same dose of PBS was used as Control when SP was not administered. After 10 days of cell culture, cell viability was measured using the WST-1 assay. Next, qRT-PCR and ICC were used to analyze the tendinopathy-related markers to determine the different effects of the duration of SP treatment.
Oh S.Y., Kim D.K., Han S.H., Lee H.H., Jeong Y., Baek M., Kim H., Ahn W, & Lee S. (2020). Sustained Exposure of Substance P Causes Tendinopathy. International Journal of Molecular Sciences, 21(22), 8633.
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2

Tenocyte Modulation by Palmitate and DEL-1

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Human tenocytes (ZenBio, USA) were cultured with tenocyte culture medium (ZenBio) supplemented with 10% fetal bovine serum (FBS) (HyClone, USA) and 1% antibiotics (100 IU/ml penicillin and 100 mg/ml streptomycin) (HyClone) at 37°C in a humidified atmosphere containing 5% CO2. The cells were cultured on collagen type I-coated (Sigma-Aldrich, USA) culture plates and rinsed with phosphate-buffered saline (PBS). Tenocytes were subcultured when they reached approximately 90 to 95% confluence. Tenocytes at passages 4 or 5 were used and confirmed to be free of contamination with mycoplasma. Sodium palmitate (Sigma-Aldrich) was conjugated with 5% bovine serum albumin (fatty acid-free grade; Sigma-Aldrich). Human recombinant DEL-1 (Abcam, USA) was reconstituted in PBS. Tenocytes were treated with bovine serum albumin (BSA)-conjugated palmitate (400 μM), DEL-1 (0 to 2 μg/ml), 3-methyladenine (3-MA) (0.5 mM) (Sigma-Aldrich), and/or 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (0.1 mM) (Sigma-Aldrich) at the same time for 24 hours. BSA was used as a vehicle control.
Park T.J., Park S.Y., Cho W., Oh H., Lee H.J., Abd El-Aty A.M., Bayram C., Jeong J.H, & Jung T.W. (2022). Developmental endothelial locus-1 attenuates palmitate-induced apoptosis in tenocytes through the AMPK/autophagy-mediated suppression of inflammation and endoplasmic reticulum stress. Bone & Joint Research, 11(12), 854-861.
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3

Paracrine Factors Influence on Tenocyte Migration

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To investigate the influence of paracrine factors secreted from MSCs cultured on electrospun sheets on the migration of tenocytes, MSC conditioned medium was collected and applied to tenocytes. MSCs were seeded on the SG7 with a density of 5000 cells/cm2 and cultured for 10 days. The conditioned medium was collected at the 4th, 7th, and 10th day of culture. The collected conditioned medium was mixed together and stored at −20oC for further use. The conditioned medium of MSCs cultured on SF, GelMA, and TCP were used as control. Human Tenocytes (Zen Bio) were seeded in 12 well plate with a density of 105 cells/well and cultured at 37 °C and 5% CO2 in high glucose DMEM (Gibco) along with 1% penicillin/streptomycin and 10% FBS. After the cells were confluent, the cell medium was removed and a uniform ~0.5 mm width scratch was created by scraping the confluent cell layers with a sterile pipette. Then, the cells were cultured in MSCs conditioned medium or fresh DMEM with 10% FBS. The images of the scratched wound were taken at predetermined time points to evaluate the migration of the cells.
Xue Y., Kim H.J., Lee J., Liu Y., Hoffman T., Chen Y., Zhou X., Sun W., Zhang S., Cho H.J., Lee J., Kang H., WonHyoung R., Chang-Moon L., Ahadian S., Dokmeci M.R., Lei B., Lee K, & Khademhosseini A. (2022). Co-electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration. Small (Weinheim an der Bergstrasse, Germany), 18(21), e2107714.
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4

Culturing Human Tenocytes

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Human tenocytes were purchased from ZenBio Inc. (Research Triangle, NC). Cells were cultured in tenocyte medium (ZenBio Inc.), following the manufacturer’s instructions.
Mao Y., John N., Protzman N.M., Kuehn A., Long D., Sivalenka R., Junka R.A., Gosiewska A., Hariri R.J, & Brigido S.A. (2022). A decellularized flowable placental connective tissue matrix supports cellular functions of human tenocytes in vitro. Journal of Experimental Orthopaedics, 9, 69.
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5

Modulation of Tenocyte Inflammatory Response

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Human tenocytes (Zen-Bio Inc., Research Triangle Park, NC, USA) were cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA). The 1 × 106 cells were incubated in medium containing 1 ng/mL IL-1β, IL-6, and IL-8 (R&D Systems, Minneapolis, MN, USA) for 24 h. In a subset experiment, cells were transfected with 50 nM miR-29a precursor, antisense oligonucleotide, or scramble control (Applied Biosystems) using Lipofectamin™ 2000 (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and followed by incubating in 1 ng/mL IL-1β for 24 h.
Ko J.Y., Lian W.S., Tsai T.C., Chen Y.S., Hsieh C.K., Kuo C.W, & Wang F.S. (2019). MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness. International Journal of Molecular Sciences, 20(22), 5742.
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6

Cultivation of Human Tenocytes

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Human tenocytes were purchased from ZenBio Inc. (Research Triangle, NC). Cells were cultured in tenocyte medium (ZenBio Inc., Research Triangle, NC), following the manufacturer’s instructions.
Mao Y., John N., Protzman N.M., Long D., Sivalenka R., Azimi S., Mirabile B., Pouliot R., Gosiewska A., Hariri R.J, & Brigido S.A. (2023). A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro. Journal of Materials Science. Materials in Medicine, 34(7), 37.
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