Attune nxt software version 4

The apoptotic effect of CA and RA was determined in OSCC OC3 cells, using a CoraLite 488-Annexin V and propidium iodide (PI) Apoptosis Kit (Proteintech, Rosemont, IL, USA) following the manufacturer’s instructions. Cells were treated with CA or RA alone or in combination with indicated concentrations, and control cells were treated with DMSO (0.02%). After 48 h and 72 h of incubation in conditioned medium, cells were washed with PBS and then resuspended in Annexin V binding buffer for Annexin V-FITC and PI labelling. Apoptotic cells were analyzed on a flow cytometer (FACScalibur, Becton Dickinson) by using an FITC signal detector and phycoerythrin emission signal detector. Attune^® NxT Software version 4.2 (Life Technologies, Carlsbad, NY, USA) was used for acquisition and data analysis.

The apoptotic effect of CA and RA was determined in OSCC OC3 cells, using a CoraLite 488-Annexin V and propidium iodide (PI) Apoptosis Kit (Proteintech, Rosemont, IL, USA) following the manufacturer’s instructions. Cells were treated with CA or RA alone or in combination with indicated concentrations, and control cells were treated with DMSO (0.02%). After 48 h and 72 h of incubation in conditioned medium, cells were washed with PBS and then resuspended in Annexin V binding buffer for Annexin V-FITC and PI labelling. Apoptotic cells were analyzed on a flow cytometer (FACScalibur, Becton Dickinson) by using an FITC signal detector and phycoerythrin emission signal detector. Attune^® NxT Software version 4.2 (Life Technologies, Carlsbad, NY, USA) was used for acquisition and data analysis.