Tissues were fixed in 4% Paraformaldehyde in PBS for 24 h, then washed with PBS to remove any remaining Paraformaldehyde. Tissues were embedded in Paraplast X-TRA paraffin wax (Sigma-Aldrich, St. Louis, MO, USA. Cat.# P3808-1KG) and chilled prior to sectioning. Tissues were sectioned using a Leica Histocore Autocut microtome.
Histocore autocut microtome
Manufactured by Leica
The Leica Histocore Autocut microtome is a precision instrument designed for cutting thin, uniform sections from paraffin-embedded tissue samples. It features an automated cutting mechanism that ensures consistent, high-quality slices for histological analysis and examination.
Automatically generated - may contain errors
4 protocols using histocore autocut microtome
1
Paraffin Embedding and Sectioning
2
Histological Analysis of Pig Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 4-week female Yorkshire pig (#2) was euthanized for a different study (Stanford APLAC protocol nr 33684), the brain was harvested, cut into 5-mm coronal slabs using a brain slicer, and a mid-frontal slab (#5) was paraffin-embedded, similar to the human pathologic specimen preparation above. The slab was cut in 10μm sections using a Leica HistoCore AUTOCUT microtome. After deparaffinization, a section (#127) was stained with hematoxylin and eosin and cover-slipped (Fig. 2J –M ).
3
Oocyte Maturation in Mice Across Ages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ovaries from CF-1 mice of varying ages (2 weeks, 3 weeks, 5 weeks, and 8 weeks) were collected, submerged, and stored in buffered zinc formalin overnight. Tissues were then moved to 70% ethanol for storage until undergoing a 10-h protocol using a Leica ASP300S enclosed tissue processor. After processing, tissues were embedded in paraffin wax and cooled fully using the Leica EG1150 C/H pair. Embedded tissues were sectioned at 8 µm thickness using a Leica Histocore AutoCut microtome. Representative samples were taken for each age by taking 3 consecutive sections periodically (every 4, 6, 8, or 10 sections respectively). Tissues were stained with Hematoxylin and Eosin (Abcam, # 245880) before being mounted with permount resin (Thermo Fisher Scientific, Inc. # SP15). Oocytes and their corresponding nucleus positioning were examined using 10X or 40X objective on a Leica DMI8 microscope.
4
Histological Analysis of Decalcified Teeth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the histological analysis, the preference was accorded to decalcified sections. The procedure followed was consistent with the protocol shown by Foster [20] . Crowns were removed with a Dremel® 3000 Rotary tool, to shorten the time required for decalcification; roots were then submerged in Osteomoll® decalcifying solution (CH₂O 4%, HCl 10%) for approximately 36-48 hours, with some of the hardest teeth requiring even 72 hours before reaching the required level of decalcification. Each root was further cut transversally to its middle third and embedded in paraffin (Leica ASP300 processor, Histoline TEC2900 incorporator). The middle third area was then cut into 10 µm sections starting from its occlusal end (Leica HistoCore auto cut microtome), for at least 3 for each sample.
The staining was performed using hematoxylin and eosin, after each section was deparaffinized in xylene for 10' and rehydrated in a descending ethanol series (10' in 100%, 5' in 90% ethanol, 5' in 70% ethanol) and rinsed in deionized water. The sections were then dipped in hematoxylin for 1.5', put in tap water for 2' and then in eosin for 1.5', then dehydrated in an ascending ethanol series (50%, 70%, and 100%) and cleared in xylene for 10' before mounting coverslips.
The staining was performed using hematoxylin and eosin, after each section was deparaffinized in xylene for 10' and rehydrated in a descending ethanol series (10' in 100%, 5' in 90% ethanol, 5' in 70% ethanol) and rinsed in deionized water. The sections were then dipped in hematoxylin for 1.5', put in tap water for 2' and then in eosin for 1.5', then dehydrated in an ascending ethanol series (50%, 70%, and 100%) and cleared in xylene for 10' before mounting coverslips.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!