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Antibodies against phospho as160 thr642

Manufactured by Merck Group

Antibodies against phospho-AS160 Thr642 are laboratory reagents that specifically recognize the phosphorylated form of the Thr642 residue on the AS160 (Akt substrate of 160 kDa) protein. AS160 is a key regulator of glucose transport and its phosphorylation status is an important indicator of insulin signaling and glucose metabolism. These antibodies can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the phosphorylation of AS160 and its role in cellular processes.

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2 protocols using antibodies against phospho as160 thr642

1

Antibody-based Analysis of Insulin Signaling

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Antibodies against phospho‐Akt Ser473 (#9271), phospho‐Akt
Thr308 (#9275), phospho‐TBC1D1 Thr590 (#6927), total Akt
(#9272), and total acetyl CoA carboxylase (ACC, #3662) were purchased from Cell
Signaling Technology (Beverly, MA). Antibodies against phospho‐AS160 Thr642(#07‐802), phospho‐ACC Ser79 (#07‐303),
phospho‐TBC1D1 Ser237 (#07‐2268), and total AS160
(#07‐741) were from Millipore (Temecula, CA). Anti‐phospho‐AS160
Ser588(#3028P2) was from Symansis Limited (Timaru, New Zealand). Anti‐GLUT4
antibody (#4670‐1704) was from Bio‐Rad AbD Serotec (Oxford, UK).
HRP‐conjugated anti‐rabbit IgG was from Biosource International (Camarillo, CA).
HRP‐conjugated anti‐sheep IgG was from Millipore. Enhanced chemiluminescence reagents
(ECL, ECL Plus, and ECL Prime) were obtained from GE Healthcare Life Sciences (Uppsala, Sweden). All
other reagents were obtained from Sigma (St. Louis, MO).
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2

Signaling Pathway Protein Detection

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Antibodies against phospho‐Akt Ser473 (#9271), phospho‐Akt Thr308 (#9275), phospho‐TBC1D1 Thr590 (#6927), phospho‐JNK Thr183/Tyr185 (#9251), phospho‐p38 MAPK Thr180/Tyr182 (#9216), phospho‐ERK1/2 Thr202/Tyr204 (#9101), total Akt (#9272), total TBC1D1 (#4629S), total‐JNK (#9252), total‐p38 MAPK (#9212), total‐ERK1/2 (#9102), total IκBα (#9242), and total‐acetyl CoA carboxylase (ACC, #3662) were from Cell Signaling Technology (Beverly, MA). Antibodies against phospho‐AS160 Thr642 (#07‐802), phospho‐ACC Ser79 (#07‐303), phospho‐TBC1D1 Ser237 (#07‐2268), and total AS160 (#07‐741) were from Millipore (Temecula, CA). Anti‐phospho‐AS160 Ser588 (#3028P2) was from Symansis Limited (Timaru, New Zealand). Anti‐GLUT4 antibody (#4670‐1704) was from Bio‐Rad AbD Serotec (Oxford, UK). Anti‐SPT2 antibody (ab23696) was from Abcam (Cambridge, MA). Horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG was from Biosource International (Camarillo, CA). HRP‐conjugated anti‐sheep IgG was from Millipore. HRP‐conjugated anti‐mouse IgG was from Santa Cruz Biotechnology. Enhanced chemiluminescence reagents (ECL, ECL plus, and ECL Prime) were obtained from GE Healthcare Life Sciences (Uppsala, Sweden). All other reagents were obtained from Sigma‐Aldrich (St. Louis, MO).
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