Cd19 pe cy7

Manufactured by BD

Sourced in United States, France

CD19-PE-Cy7 is a fluorescent-conjugated antibody used for the detection and enumeration of CD19-positive cells in flow cytometry analysis. It is composed of a phycoerythrin-cyanine 7 (PE-Cy7) fluorescent dye conjugated to an anti-CD19 monoclonal antibody. CD19 is a cell surface antigen expressed on B lymphocytes and is commonly used as a marker for identifying and quantifying B cells in various biological samples.

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Lab products found in correlation

Cd3 fitc, by BD (8 mentions) Facsaria 2, by BD (7 mentions) Facsdiva software, by BD (7 mentions) Cd8 apc cy7, by BD (7 mentions) Cd56 apc, by BD (7 mentions) Cd8 apc h7, by BD (6 mentions) Cd24 pe, by BD (6 mentions) Cd8 fitc, by BD (6 mentions) Cd3 pe, by BD (6 mentions) Facsaria, by BD (6 mentions) Cd45ro apc, by BD (6 mentions) Cd3 bv421, by BD (5 mentions) Cd4 fitc, by BD (5 mentions) Igm fitc, by BD (5 mentions) Cd3 apc, by BD (5 mentions) Il 17 apc, by R&D Systems (5 mentions) Cd25 pe cy7, by BD (5 mentions) Cd3 percp, by BD (5 mentions) Cd90 apc, by BD (5 mentions) Cd4 pe, by BD (4 mentions)

Close spelling variants of this product

CD19-PE (90 citations) Anti-CD19-PE-Cy7 (37 citations) PE-Cy7-conjugated anti-CD19 (6 citations) CD19-PE-Cy7 (SJ25C1), (4 citations) CD19 PE-Cy7 (clone SJ25C1, (3 citations) Anti-mouse CD19 PE/Cy7, (2 citations) PE-Cy7-conjugated anti-human CD19 (2 citations) PE-Cy7-α-CD19 (1D3, (2 citations) PE-Cy7-labeled CD19 (2 citations)

83 protocols using cd19 pe cy7

Immune Cell Profiling in Vedolizumab Response

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Absolute counts of T cells, B cells, NK cells and monocytes were determined by flow cytometry (BD FacsCanto II cytometer with DIVA software) and Sysmex XS-800i analyzer in responder and non-responder patients at inclusion, before the first infusion of vedolizumab, and at week 22. In the same way, α4β7 expression was analyzed by flow cytometry using a FITC-conjugated vedolizumab antibody in the same cells and in B cell subpopulations i.e. Plasmablasts, Transitional B cells, Switch and non-Switch B cells, Naïve B cells.
Within a maximum of 4h after drawing, EDTA whole blood samples (50μl) were incubated 15min with the following antibodies to analyze T cells, B cells, monocytes and NK cells: CD45-KrOrange, CD16-PE, CD14-PECy5, IgG1-FITC (Beckman Coulter, France) and with CD3-BV421, IgG1-APC, CD56-PE, CD19-PECy7, CD8-APC-H7 (BD Biosciences, France). For B cells subpopulation analysis, the following antibodies were used for staining 100μl of washed blood: CD45-KrOrange, IgG1-FITC (Beckman Coulter) and CD24-PE, CD27 PerCP-Cy5, CD38-APC, IgD-APC-H7, CD19-PECy7 (BD Biosciences). Quantitation of lymphocyte subsets (B cells, T cells, CD4 and CD8 T cells and NK cells) was performed using flow cytometry with BD Trucount™ Tubes (BD Biosciences).

Quénéhervé L., Trang-Poisson C., Fantou A., Flamant M., Durand T., Bouguen G., Bregeon J., Oullier T., Amil M., Dewitte M., Bardot S., Blandin S., Braudeau C., Vibet M.A., Josien R., Neunlist M, & Bourreille A. (2024). Confocal laser endomicroscopy as predictive biomarker of clinical and endoscopic efficacy of vedolizumab in ulcerative colitis: The DETECT study. PLOS ONE, 19(4), e0298313.

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Multicolor Flow Cytometry for Immune Cell Analysis

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Blood was collected with heart puncture and transferred into a Na EDTA-containing tube. Single cell suspensions were prepared from spleens and BM as described (Heo, 2005 ). Whole blood (50 μl) was stained and erythrocytes were lysed with FACSlyse (BD Biosciences, San Jose) before analysis. Splenocytes and BM cells were counted, and 1 × 10⁶ cells were used for staining followed by FACSlyse and analysis by six-color flow cytometry with a FACSCanto flow cytometer (BD Biosciences), and the following fluorochrome conjugated Abs: PerCP Cy5.5-CD45, APC Cy7-CD45, PerCP Cy5.5-CD4, PE-CD4, FITC-CD4, FITC-CD8a, PE-CD8a, PerCP-CD8a, FITC-CD3, APC-CD3e, PerCP-CD3e, PE Cy7-CD24, FITC-CD43, PerCP-CD249- APC Cy7-B220, PE -IgD, APC-IgM, APC Cy7-CD3, PE Cy7-CD19, PerCP cy5.5-CD93, APC-CD138, PE-CXCR4, FITC-MHC-II, PE-CD19, FITC-CD19, PE Cy7-CD19, APC-CXCR5, PE Cy7-PD1, PerCP Cy5.5-CD138 and anti-CD16/32 (Fc block). The Abs were purchased from BD Pharmingen (San Diego, CA), Biolegend (San Diego, CA), or eBiosciences (Thermo Fisher Scientific, MA, USA). Frequencies and numbers of populations in the blood and spleens of B6 and BTBR mice were gated based on FSC-A and SSC-A, followed by gating out the doublets and finally gated on CD45⁺ cell. Data were analyzed by Flow Jo-V10.

Uddin M.N., Yao Y., Mondal T., Matala R., Manley K., Lin Q, & Lawrence D.A. (2020). Immunity and autoantibodies of a mouse strain with autistic-like behavior. Brain, Behavior, & Immunity - Health, 4, 100069.

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Isolation and Phenotyping of Memory B Cells

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Peripheral blood lymphocytes were isolated from heparinized blood by Lymphoprep gradient centrifugation (Nicamed, Oslo, Norway). For detecting IgM memory B cells and switched memory B cells, peripheral blood cells were stained with the appropriate antibody combination, including IgM FITC, CD24 PE, CD38 PERCP Cy5.5, CD19PECy7, CD27 APC, IgG APC H7, IgD V450, CD45 V500C (Becton Dickinson Co., San Jose, CA). All flow cytometric analyses were performed on a FACSLyric system interfaced to a BD FACSuite (Becton Dickinson Co.) computer program. Dead cells were excluded from analysis by side/forward scatter gating. The lower limit of normal for IgM memory B cells was 26/µl, as previously reported^{31 (link)}. Gating strategies for the detection of lymphocyte subpopulations are depicted in Supplementary Fig. 1.

Lenti M.V., Aronico N., Pellegrino I., Boveri E., Giuffrida P., Borrelli de Andreis F., Morbini P., Vanelli L., Pasini A., Ubezio C., Melazzini F., Rascaroli A., Antoci V., Merli S., Di Terlizzi F., Sabatini U., Cambiè G., Tenore A., Picone C., Vanoli A., Arcaini L., Baldanti F., Paulli M., Corazza G.R, & Di Sabatino A. (2020). Depletion of circulating IgM memory B cells predicts unfavourable outcome in COVID-19. Scientific Reports, 10, 20836.

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Immunophenotyping of Peripheral Blood Cells

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On peripheral blood, after red blood cell lysis with ammonium chloride, the following antibodies were employed: CD3 PerCP (clone BW264/56, MiltenyiBiotec, BergischGladbach, Germany), CD4 APC (clone OKT4, Becton Dickinson, Franklin Lakes, NJ, USA), CD8 PE-Cy7 (clone RPA-T8, Becton Dickinson, USA), TCR alpha–beta APC (clone T10B9, Becton Dickinson), TCR gamma-delta FITC (11F3, MiltenyiBiotec, DE), CD45RA APC-H7 (clone T6D11, MiltenyiBiotec, DE), CCR7 PE (clone 3D12, Ebioscience, San Diego, CA, USA), CD127 PE-CY7 (clone eBioRDR5, eBiosciences), CD16 PE (clone 3G8), CD56 PE (clone NCAM16.2), CD19 PE-CY7 (clone SJ25C1, Becton Dickinson), CD27 FITC (clone M-T271, Becton Dickinson), CXCR5 (clone J252D4 Biolegend), CD31 (cloneWM59, Miltenyi Biotec, DE).

Baccelli F., Leardini D., Muratore E., Messelodi D., Bertuccio S.N., Chiriaco M., Cancrini C., Conti F., Castagnetti F., Pedace L., Pession A., Yoshimi A., Niemeyer C., Tartaglia M., Locatelli F, & Masetti R. (2022). Immune dysregulation associated with co-occurring germline CBL and SH2B3 variants. Human Genomics, 16, 40.

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T Cell Identification and Transduction

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The following antibodies were used for basic T cell identification: CD3-PE, CD8-FITC, CD14-PE-Cy7CD16-PE-Cy7, CD19-PE-Cy7, all from Becton Dickinson. To detect transduced NYESO-1 TCR expressing cells, APC-conjugated dextramer reagents specific for the HLA-A*0201 / SLLMWITQV complex (Immudex, Copenhagen Denmark) were utilized at the manufacturer recommended concentrations.

Rapoport A.P., Stadtmauer E.A., Binder-Scholl G.K., Goloubeva O., Vogl D.T., Lacey S.F., Badros A.Z., Garfall A., Weiss B., Finklestein J., Kulikovskaya I., Sinha S.K., Kronsberg S., Gupta M., Bond S., Melchiori L., Brewer J.E., Bennett A.D., Gerry A.B., Pumphrey N.J., Williams D., Tayton-Martin H.K., Ribeiro L., Holdich T., Yanovich S., Hardy N., Yared J., Kerr N., Philip S., Westphal S., Siegel D.L., Levine B.L., Jakobsen B.K., Kalos M, & June C.H. (2015). NY-ESO-1 specific TCR engineered T-cells mediate sustained antigen-specific antitumor effects in myeloma. Nature medicine, 21(8), 914-921.

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T Cell Identification and Transduction

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Mesenchymal Stem Cell Immunophenotyping

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To evaluate the MSC immunophenotype a flow cytometer analysis was performed at passage 4. After the detachment, cells where washed with Dulbecco’s phosphate buffered saline (DPBS 1× without Ca²⁺/Mg²⁺; Euroclone) and briefly incubated with the following conjugated monoclonal antibodies and isotype controls for 15 min at room temperature: CD90 FITC, CD105 PE, CD73 PE, HLA-DR PE-Cy7, CD45 APC-Cy7, CD14 APC-Cy7, CD19 PE-Cy7,CD34 APC (Becton Dickinson), APC-Cy7 IGg₁ isotype control, APC-Cy7 IGg₂ isotype control, PE-Cy7 IGg₁ isotype control, PE-Cy7 IGg₂ isotype control, FITC IGg₁ isotype control, PE IGg₁ isotype control and APC IGg₁ isotype control (BD Pharmingen). The vitality dye 7-aminoactinomycin D (7-AAD; BD Pharmingen) was also added to discriminate dead cells during flow cytometry analysis. After incubation, cells were washed, resuspended in DPBS and then analyzed by flow cytometry on a FACS Canto II (Becton Dickinson) equipped with the DIVA software program.

Becherucci V., Piccini L., Casamassima S., Bisin S., Gori V., Gentile F., Ceccantini R., De Rienzo E., Bindi B., Pavan P., Cunial V., Allegro E., Ermini S., Brugnolo F., Astori G, & Bambi F. (2018). Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience. Stem Cell Research & Therapy, 9, 124.

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Multicolor Flow Cytometry Analysis of Lymphocyte Subsets

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Multicolour flow cytometric analysis was performed using the following fluorochrome-labelled anti-human monoclonal antibodies for measuring lymphocyte Th and Tfh subsets (CD4-APC-Vio770, CD45RA-PE-Vio770, CCR6-APC, CXCR3-VioBright FITC CXCR5 PerCP-Cy5.5, CCR4-PE) all from Miltenyi biotech. Tregs and Tfr were detected using BD Human Regulatory T Cell Cocktail (BD, San Jose, CA, USA), including CD4-FITC (clone SK3), CD25-PECy7 (clone 2A3), CD127 Alexa Fluor® 647 and adding CXCR5 PerCP-Cy5 and CD8 horizon450 (the last two from Biolegend). Bregs were detected using the following fluorochrome-labelled anti-human monoclonal antibodies: CD38-FITC CD1d-PE, CD19-PE-Cy7, CD5-PerCP-CY5.5, CD24-APC-H7 and CD27- BV510, all from BD Biosciences (San Jose, CA, USA).

Bergantini L., d’Alessandro M., Cameli P., Pianigiani T., Fanetti M., Sestini P, & Bargagli E. (2021). Follicular T Helper and Breg Cell Balance in Severe Allergic Asthma Before and After Omalizumab Therapy. Molecular Diagnosis & Therapy, 25(5), 593-605.

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Comprehensive Immunophenotyping of Bone Marrow

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Characterization of human samples was performed using the EuroFlow lyse-wash-and-stain using a standard sample preparation protocol adjusted to 10⁶ BM-derived nucleated cells, together with the eight-color combination of the monoclonal antibodies CD138-BV421, CD27-BV510, CD38-FITC, CD56-PE, CD45-PerCPCy5.5, CD19-PECy7, CD117-APC and CD81-APCH7 (BD Biosciences)^{67 (link)}. Data acquisition was performed in a FACS CantoII flow cytometer (BD Biosciences). Samples were analyzed using the Infinicyt software (Cytognos SL) and the semiautomated pipeline ‘FlowCT’, based on the analysis of multiple files by automated cell clustering^{68 (link)}. Cell sorting was performed in a FACS Aria sorter instrument. Classification of BM samples according to immune cell infiltration was calculated similar to that in the mouse samples. The maximum percentages of T cells and NK cells present in the BM from healthy control individuals (cutoff, 20%) were used to divide patients with MM into cases with low or high number of immune-infiltrating cells.

Larrayoz M., Garcia-Barchino M.J., Celay J., Etxebeste A., Jimenez M., Perez C., Ordoñez R., Cobaleda C., Botta C., Fresquet V., Roa S., Goicoechea I., Maia C., Lasaga M., Chesi M., Bergsagel P.L., Larrayoz M.J., Calasanz M.J., Campos-Sanchez E., Martinez-Cano J., Panizo C., Rodriguez-Otero P., Vicent S., Roncador G., Gonzalez P., Takahashi S., Katz S.G., Walensky L.D., Ruppert S.M., Lasater E.A., Amann M., Lozano T., Llopiz D., Sarobe P., Lasarte J.J., Planell N., Gomez-Cabrero D., Kudryashova O., Kurilovich A., Revuelta M.V., Cerchietti L., Agirre X., San Miguel J., Paiva B., Prosper F, & Martinez-Climent J.A. (2023). Preclinical models for prediction of immunotherapy outcomes and immune evasion mechanisms in genetically heterogeneous multiple myeloma. Nature Medicine, 29(3), 632-645.

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Phenotypic Analysis of PBMCs

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Isolated PBMCs were examined for cell surface expression by flow cytometry. Expression of surface markers was determined using the following antibodies: CD34 BV421 (BD, clone 581), CD10 APC (BioLegend, clone HI10a), CD123 BV711 (BD, clone 7G3), CD3 FITC (BioLegend, clone HIT3a), CD90 PE (BD, clone 5E10), CD38 CF594 (BD, clone HIT2), CD19 PECy7 (BD, clone HIB19), CD20 BV605 (BD, clone 2H7), and CD45RA A700 (BD, clone HI100). Cells were stained for 30 minutes at 4°C. Single-color controls were obtained using CompBead Anti-Mouse Ig, k/Negative Control Compensation Particles (BD Biosciences). Data were acquired on BD FACSAria Cell Sorter and analyzed with FlowJo software (BD Biosciences).

Fisher M.H., Kirkpatrick G.D., Stevens B., Jones C., Callaghan M., Rajpurkar M., Fulbright J., Cooper M.A., Rowley J., Porter C.C., Gutierrez-Hartmann A., Jones K., Jordan C., Pietras E.M, & Di Paola J. (2020). ETV6 germline mutations cause HDAC3/NCOR2 mislocalization and upregulation of interferon response genes. JCI Insight, 5(18), e140332.

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