FTIR analysis was used to determine the specific chemical composition of pure PLA, PVA and PLA-PVA core-shell structures and PLA-PVA-CTGF core-shell structures. The FTIR measurements were conducted using a PerkinElmer (USA), Spectrum 400 FTIR instrument.
Spectrum 400 ftir instrument
Manufactured by PerkinElmer
Sourced in United States
The Spectrum 400 FTIR instrument is a Fourier Transform Infrared (FTIR) spectrometer designed for analytical applications. It utilizes infrared light to detect and identify chemical compounds based on their unique molecular vibrations. The instrument measures the absorption of infrared radiation by a sample, producing a spectrum that can be used for qualitative and quantitative analysis.
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Lab products found in correlation
6545 quadrupole time of flight, by Agilent Technologies (1 mentions)
Unity plus 500 spectrometer, by Agilent Technologies (1 mentions)
Dmx 500 spectrometer, by Bruker (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
Silica gel 60 f254 plate, by Merck Group (1 mentions)
4 protocols using spectrum 400 ftir instrument
1
FTIR Analysis of PLA, PVA, and Composites
2
Detailed Spectroscopic Analysis of Organic Compounds
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All chemicals and reagents were purchased from available
commercial
companies and were used without further purification. Reactions were
monitored by thin-layer chromatography using pre-coated aluminum sheets
(silica gel 60 F254, 0.2 mm thickness). Formation of the
desired product was confirmed by infrared (IR), 1H NMR, 13C NMR, and mass spectra (LC–MS/HRMS). IR spectra were
recorded on a Perkin Elmer Spectrum 400 FTIR instrument, and the frequencies
are expressed in cm–1. 1H NMR and 13C NMR spectra were recorded on a Bruker Avance II-400 spectrometer
in CDCl3, and chemical shifts were recorded in ppm with
TMS as the internal standard. The HMBC spectrum was recorded on an
ECZR series 600 MHz NMR spectrometer (Jeol, Japan). Melting points
were recorded by an open capillary tube method. Mass spectral data
were obtained with a Waters UPLC-TQD mass spectrometer (ESI-MS). High-resolution
mass spectra (ESI-HRMS) were recorded on an Agilent 6545 Quadrupole
Time-of-Flight. All reactions were purified by column chromatography
over silica gel (100–200 mesh) using ethyl acetate and hexane
as the eluent.
commercial
companies and were used without further purification. Reactions were
monitored by thin-layer chromatography using pre-coated aluminum sheets
(silica gel 60 F254, 0.2 mm thickness). Formation of the
desired product was confirmed by infrared (IR), 1H NMR, 13C NMR, and mass spectra (LC–MS/HRMS). IR spectra were
recorded on a Perkin Elmer Spectrum 400 FTIR instrument, and the frequencies
are expressed in cm–1. 1H NMR and 13C NMR spectra were recorded on a Bruker Avance II-400 spectrometer
in CDCl3, and chemical shifts were recorded in ppm with
TMS as the internal standard. The HMBC spectrum was recorded on an
ECZR series 600 MHz NMR spectrometer (Jeol, Japan). Melting points
were recorded by an open capillary tube method. Mass spectral data
were obtained with a Waters UPLC-TQD mass spectrometer (ESI-MS). High-resolution
mass spectra (ESI-HRMS) were recorded on an Agilent 6545 Quadrupole
Time-of-Flight. All reactions were purified by column chromatography
over silica gel (100–200 mesh) using ethyl acetate and hexane
as the eluent.
3
Characterization of Natural Product Derivatives
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Melting points were determined using a Fisher-Johns apparatus and are uncorrected. Infrared spectra were obtained in the range of 4000 to 400 cm−1 using a Spectrum 400 FT-IR instrument (Perkin Elmer San Jose, CA, USA). NMR spectra including NOE differential, COSY, HMBC and HMQC experiments were recorded in CDCl3 or CD3OD on a Unity Plus 500 spectrometer (Varian, Palo Alto, CA, USA) or on a DMX500 spectrometer (Bruker, Billerica, MA, USA) operating at 500 or 300 MHz (1H) or 125 or 75 MHz (13C) NMR, using tetramethylsilane as an internal standard. Open column chromatography was carried out on silica gel 60 (70–230 mesh; Merck, Darmstadt, Germany) or Sephadex LH-20 (Sigma-Aldrich Chemical). TLC analyses were performed on silica gel 60 F254 plates (Merck) and spots were visualized by spraying with ceric sulfate (10%) solution in H2SO4, followed by heating. Semi-preparative RP-HPLC was carried out using a Waters (Milford, MA, USA) HPLC instrument equipped with Waters 996 UV photodiode array detector (900) set at 270–400 nm, a Purospher® Star RP-18 endcapped column (10 mm i.d. × 250 mm, 5 μm) and isocratic conditions (CH3CN–H2O 48:52; flow: 2.8 mL/min).
4
FT-IR Spectroscopy of Diverse Samples
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FT-IR spectra
were recorded using a PerkinElmer Spectrum 400 FT-IR instrument, equipped
with attenuated total reflectance with a diamond crystal. For all
the analyses, background data collection was performed before sample
measurements. All spectra were acquired in the range of 650–4000
cm–1 with 16 scans at a resolution of 4 cm–1.
were recorded using a PerkinElmer Spectrum 400 FT-IR instrument, equipped
with attenuated total reflectance with a diamond crystal. For all
the analyses, background data collection was performed before sample
measurements. All spectra were acquired in the range of 650–4000
cm–1 with 16 scans at a resolution of 4 cm–1.
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