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Aperio xt slide scanner

Manufactured by Leica
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The Aperio XT slide scanner from Leica is a high-performance digital pathology imaging system designed for clinical and research applications. The Aperio XT is capable of capturing high-resolution digital images of tissue slides at various magnification levels, enabling efficient and accurate analysis of microscopic samples.

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Lab products found in correlation

Aperio imagescope, by Leica (3 mentions) Ab11174, by Abcam (2 mentions) Imagescope software, by Leica (2 mentions) Ultravision lp detection system hrp polymer dap plus chromogen, by Thermo Fisher Scientific (2 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) Ab206386, by Abcam (1 mentions) Decloaking chamber, by Biocare Medical (1 mentions) Ab38148, by Abcam (1 mentions) γ h2ax, by Abcam (1 mentions)

Spelling variants of this product

Aperio slide scanner (83 citations) Slide scanner (31 citations) Aperio ScanScope XT Slide Scanner (30 citations) Aperio XT (21 citations) Aperio ScanScope XT Slide Scanner system (3 citations) Aperio XT scanner (2 citations) Aperio scanners (2 citations)

8 protocols using aperio xt slide scanner

1

Immunohistochemical Assessment of MMR Proteins

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Tissue microarrays (TMAs) were constructed using three individual 0.6 mm cores of formalin-fixed paraffin-embedded tumour tissue for each patient. Sections were taken from the TMAs and stained for the MMR proteins, hMLH-1 and hMSH-2, using pre-determined immunohistochemical protocols. The glass slides were scanned using an Aperio XT slide scanner (Aperio Technologies Inc, San Diego, CA, USA) and visualised using Aperio ImageScope (Aperio Technologies Inc). Each individual tumour core was scored as positive or negative, with negative defined as the absence of any staining within tumour cells. Staining within immune cells was used as an internal positive control. Cases scored as negative for either marker were taken to be MMR-deficient cancers. In cores with no tumour tissue, assessment of MMR status was not possible and these were classed as unknown.
Pine J.K., Morris E., Hutchins G.G., West N.P., Jayne D.G., Quirke P, & Prasad K.R. (2015). Systemic neutrophil-to-lymphocyte ratio in colorectal cancer: the relationship to patient survival, tumour biology and local lymphocytic response to tumour. British Journal of Cancer, 113(2), 204-211.
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2

Immunohistochemistry and Immunofluorescence Protocol

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Tumors were fixed in 10% neutral buffered formalin for 24hrs, and transferred to cold 70% ethanol. Samples were paraffin-embedded and sectioned at 4um. Sections were de-paraffinized with xylene, and antigen retrieval was performed in 10mM Citrate buffer (pH6) using a pressure cooker. Endogenous peroxidase was quenched with 3% Hydrogen Peroxide. Sections were blocked with 10% Power Block agent (BioGenex) in PBS for 10 mins, and incubated with primary antibody overnight at 4°C. Subsequently three PBS washes, sections were incubated with secondary antibody (Vector Elite) for 1hr at room temperature. For immunohistochemistry, staining was visualized with Vectastain ABC kit (Vector Laboratories) per manufacturer’s instructions follow by Hematoxylin counterstaining. For immunofluorescence, staining was visualized with either Tyraminde Signal Amplification reagent (Thermo Fisher Scientific) for 10 minutes per manufacturer’s instructions, followed by DAPI stain for 5 minutes. Immunohistochemistry images were acquired using Aperio-XT Slide scanner (Aperio Technologies). Immunofluorescent images were acquired using an LSM510 Confocal Microscope (Carl Zeiss), and analyzed using ZEN software.
Xiao B., Zuo D., Hirukawa A., Cardiff R.D., Lamb R., Sonenberg N, & Muller W.J. (2020). Rheb1-independent Activation of mTORC1 in Mammary Tumors Occurs Through Activating Mutations in mTOR. Cell reports, 31(4), 107571.
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3

Immunohistochemical Analysis of Tissue Samples

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Four-micron sections of paraffin-embedded tissues were stained with Mayer's H&E Y/erythrosine B staining, Ki67 (0.084 μg/ml; 12202S; Cell Signaling Technologies, Danvers, MA, USA), and γH2A.X (0.06 μg/ml; ab11174; Abcam, Cambridge, UK) as described earlier (6 (link), 24 (link)) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis by immunohistochemical staining [ab206386; Abcam, Cambridge, UK) with Mayer's hematoxylin nuclear stain. Slides were imaged using an Aperio XT slide scanner and captured using Aperio ImageScope (Leica Biosystems, Wetzlar, Germany), and five high-power images were assessed per sample (N = 4–6 brains per treatment group] using ImmunoRatio (Seinajoki, Finland) as described earlier (24 (link)).
McKelvey K.J., Wilson E.B., Short S., Melcher A.A., Biggs M., Diakos C.I, & Howell V.M. (2021). Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma. Frontiers in Oncology, 11, 633210.
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4

Xenograft Specimen Processing and Imaging

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Processing of xenograft specimens, including paraffin embedding, sectioning, and all staining, was performed by the Mutant Mouse Pathology Laboratory at the Center for Comparative Medicine at the University of California, Davis. Four micrometer thick paraffin sections were stained with Mayer's hemotoxylin and eosin or immunostained as described previously,[42 (link)] with some antibody-dependent and empirical based modifications; e.g., lot-based differences in antibody dilutions. Antigen retrieval was performed at 125°C in pH 6.0 citrate buffer, using a Decloaking Chamber (Biocare Medical, Concord, CA) pressurized to 15psi. The total incubation time was 45 min. Antibodies used for immunohistochemistry are provided in Supplementary Supplementary Table 1. Specimens were incubated with primary antibodies overnight at room temperature in a humidified chamber. Slides were scanned at 20× magnification using an Aperio® XT slide scanner (Leica Biosystems), imported into the Aperio Spectrum database, and visualized with Aperio® Imagescope software.
Hines W.C., Kuhn I., Thi K., Chu B., Stanford-Moore G., Sampayo R., Garbe J.C., Stampfer M., Borowsky A.D, & Bissell M. (2015). 184AA3: A Xenograft Model of ER+ Breast Adenocarcinoma. Breast cancer research and treatment, 155(1), 37-52.
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5

Immunohistochemical Staining of KIF5B

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Immunohistochemical staining was performed to paraffin embedded slides. After deparaffinization and rehydration slides were immersed in retrieval solution (sodium citrate 10 mM, pH 6.0 buffer). The slides were incubated in hydrogen peroxide block s, followed by Ultra V Block. Slides were incubated with a rabbit anti-KIF5B Antibody. UltraVision LP Detection System HRP Polymer & DAP Plus Chromogen (Thermo Fisher Scientific, Fremont CA) was used for detection. The TMA slides were scanned using Aperio XT slide scanner (Leica Biosystems).
Moamer A., Hachim I.Y., Binothman N., Wang N., Lebrun J.J, & Ali S. (2019). A role for kinesin-1 subunits KIF5B/KLC1 in regulating epithelial mesenchymal plasticity in breast tumorigenesis. EBioMedicine, 45, 92-107.
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6

Histologic Slide Scanning and Analysis

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Three H&E-stained 10 μm step sections per sample were scanned using an Aperio XT slide scanner (Leica Biosystems) and analyzed using ImageScope software (Leica Biosystems).
Nandi I., Smith H.W., Sanguin-Gendreau V., Ji L., Pacis A., Papavasiliou V., Zuo D., Nam S., Attalla S.S., Kim S.H., Lusson S., Kuasne H., Fortier A.M., Savage P., Martinez Ramirez C., Park M., Katzenellenbogen J.A., Katzenellenbogen B.S, & Muller W.J. (2023). Coordinated activation of c-Src and FOXM1 drives tumor cell proliferation and breast cancer progression. The Journal of Clinical Investigation, 133(7), e162324.
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7

Immunohistochemical Analysis of CPSF6 and SFPQ

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Paraffin embedded slides were applied for immunohistochemical staining. Slides were baked for 30 min at 55C, followed by deparaffinization then rehydration. Antigen retrieval was performed in sodium citrate 10 mM, pH 6.0 buffer. The slides were incubated in hydrogen peroxide block for 10 min, followed by Ultra V Block for 5 min. Slides were incubated with a rabbit anti-CPSF6 monoclonal Antibody (abcam#ab175237) or rabbit anti-SFPQ polyclonal Antibody (abcam#ab38148). UltraVision LP Detection System HRP Polymer & DAP Plus Chromogen (Thermo Fisher Scientific, Fremont CA) was used for detection. The TMA slides were scanned using Aperio XT slide scanner (Leica Biosystems).
Binothman N., Hachim I.Y., Lebrun J.J, & Ali S. (2017). CPSF6 is a Clinically Relevant Breast Cancer Vulnerability Target: Role of CPSF6 in Breast Cancer. EBioMedicine, 21, 65-78.
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8

Quantitative Immunohistochemistry of Brain Tissues

Cited 1 time
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Four micron section of paraffin embedded tissues were stained with Mayer’s hemotoxylin and eosin Y/erythrosin B staining, and Ki67 (0.084 μg/ml; 12,202; Cell Signalling Technologies, Danvers, MA, USA), γH2A.X (0.06 μg/ml; ab11174; Abcam, Cambridge, UK) and CD45 (0.019 μg/ml; 70,257; Cell Signalling Technologies, Danvers, MA, USA) and counterstained with Mayer’s hematoxylin nuclear stain as previously described [21 (link)]. Slides were imaged using an Aperio XT slide scanner, captured using Aperio ImageScope (Leica Biosystems, Wetzlar, Germany), and five high power images were assessed per sample (N = 6 brains per treatment group) using ImmunoRatio plugin (Seinajoki, Finland) for the open-source platform Fiji/ImageJ as previously described [17 ].
McKelvey K.J., Hudson A.L., Donaghy H., Stoner S.P., Wheeler H.R., Diakos C.I, & Howell V.M. (2022). Differential effects of radiation fractionation regimens on glioblastoma. Radiation Oncology (London, England), 17, 17.
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