Kidney tissues were fixed in 10% neutral buffered formalin and embedded in paraffin. Four-micron sections were cut for haematoxylin and eosin (HE, Yuanye Biotechnology, Shanghai, China) and Masson’s trichrome (Yuanye Biotechnology) staining according to the manufacturer’s protocol. Slides were examined and pictures were taken using a DM4000B LED Microscope System (Leica Microsystems, Germany) and a DFC 420C 5 M Digital Microscope Camera (Leica Microsystems). Tubulointerstitial damage and the degree of interstitial collagen deposition were graded as described previously [25 (link)].
Masson s trichrome
Manufactured by Yuanye Bio-Technology
Sourced in China
Masson's trichrome is a histological staining technique used to visualize the collagen fibers in tissue samples. It specifically stains collagen fibers blue, while other cellular components are stained in different colors, allowing for clear differentiation of the tissue structure.
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3 protocols using masson s trichrome
1
Histological Analysis of Kidney Tissues
2
Histological Analysis of Kidney Tissue
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Kidney specimens fixed in formalin and embedded in paraffin were cut into 4 μm sections and stained with periodic acid-Schiff (PAS, Yuanye Biotechnology, Shanghai, China), Hematoxylin-Eosin (HE, Yuanye Biotechnology), and Masson's trichrome (Yuanye Biotechnology). Slides were examined and pictures taken using a DM4000 B LED microscope system (Leica Microsystems, Germany) and a DFC 420C 5M digital microscope camera (Leica Microsystems). Tubulointerstitial damage and the degree of interstitial collagen deposition were graded as described previously [17 (link)].
3
Histological Analysis of Wound Healing
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The tissue samples harvested on day 1, 3, 7, 10, and 14 postwounding were fixed in 4% paraformaldehyde overnight at 4 °C. After being washed with PBS, they were dehydrated in a graded ethanol series (30, 50, 70, 80, 90, and 100%), xylene, and paraffin washes and embedded in paraffin. Sections (4- to 6-mm-thick) were prepared from the paraffin-embedded wound tissues and then stained with hematoxylin and eosin as well as Masson’s trichrome (Yuanye, Shanghai, CHN). Tissue sections were observed using a microscope (Olympus Microscopes, Tokyo, Japan).
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