All solvents used in this study were of HPLC grade. Acetonitrile, methanol, phosphoric acid, and trimethylamine (99.5%) were purchased from Merck (Darmstadt, Germany). Glycerol, D(+)-glucose, L-proline (99%), glycine (99%), l -phenylalanine (99%), and creatinine were obtained from R&M (R&M Marketing, Essex, U.K). 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was obtained from Toronto Research Chemicals (Toronto, Canada). A stock solution of 100 ppm PhIP standard was prepared in methanol and kept at 4 °C.
Trimethylamine
Manufactured by Merck Group
Sourced in United States, Germany, Spain, United Kingdom, Italy, China, Belgium
Trimethylamine is a colorless, flammable gas with a fishy odor. It is commonly used as a reagent in organic synthesis and as a precursor for other chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (14 mentions)
Methanol, by Merck Group (14 mentions)
Chloroform, by Merck Group (13 mentions)
Ethanol, by Merck Group (9 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Dichloromethane, by Merck Group (8 mentions)
Acryloyl chloride, by Merck Group (8 mentions)
Sodium borohydride, by Merck Group (7 mentions)
Dimethylamine, by Merck Group (7 mentions)
Trypsin edta, by Thermo Fisher Scientific (7 mentions)
Acetic acid, by Merck Group (6 mentions)
Sodium hydroxide (naoh), by Merck Group (6 mentions)
Hydrochloric acid (hcl), by Merck Group (6 mentions)
Tetrahydrofuran, by Merck Group (6 mentions)
Bovine serum albumin (bsa), by Merck Group (6 mentions)
N n dimethylformamide (dmf), by Merck Group (6 mentions)
Phosphoric acid, by Merck Group (5 mentions)
Ammonium acetate, by Merck Group (5 mentions)
4 dimethylaminopyridine, by Merck Group (5 mentions)
Hydrogen peroxide, by Merck Group (5 mentions)
131 protocols using trimethylamine
1
HPLC Analysis of PhIP in Samples
2
Cinchona Alkaloid Quantification by SFC
3
Metabolite Extraction and Analysis
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Deionized water was produced in‐house with a MilliQ® Integral 3 instrument (Millipore, Billerica, MA, USA). Acetonitrile (ACN; ≥99.9%) and methanol (MeOH; ≥99.9%) were purchased from VWR International (Vienna, Austria). n‐Hexane (≥99.0%), trimethylamine (≥99.0%), acetic acid (glacial; ≥99.0%), N‐methyl‐N‐trimethylsilyltrifluoroacetamide (MSTFA; ≥98.5%), NaOH (≥97.0%) and CDTA (≥97.0%) were obtained from Merck (Darmstadt, Germany); helium (≥99.9999) came from SIAD (St Pantaleon, Austria).
4
Cinchona Alkaloid Quantification by SFC
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Six Cinchona alkaloids (compounds 1 -6 , see Fig. 1 for structures) with a purity ≥98% were available as standards; they were purchased from Phytolab (Vestenbergsreuth, Germany; compounds 1 and 2 ) and Sigma Aldrich (St. Louis, MO, USA; compounds 3 -6 ). Plant samples (CC-2017-1 to CC-2017-4) were bought 2017 in different pharmacies in Innsbruck, Austria; voucher specimens are deposited at the Institute of Pharmacy, Pharmacognosy, University of Innsbruck. Compressed carbon dioxide for SFC analysis had a purity of ≥99.995% (4.5 grade) and came from Messer (Gumpoldskirchen, Austria). All solvents and reagents (methanol, acetonitrile, diethylamine, trimethylamine, sodium hydroxide, acetic acid, ammonium acetate, phosphoric acid) utilized in this study were of analytical grade and purchased from Merck (Darmstadt, Germany). An Arium 611 water purification system from Sartorius (Göttingen, Germany) produced the required HPLC grade water.
5
Synthesis of 2-Deoxy-D-ribose Derivatives
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2-Deoxy-D-ribose was purchased from Biosynth Ltd. (Compton, UK). All amines: trimethylamine, N,N-dimethylhexylamine, N,N-dimethyloctylamine, N,N-dimethyldecylamine, pyridine, 2-methylpyridine, 4-(N,N-dimethylamino)pyridine and isoquinoline were purchased from Merck (Darmstadt, Germany).
6
HPLC Quantification of Microbial GABA
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GABA produced after fermentation was measured using HPLC (Agilent Tech, Waldron, Germany). The HPLC had Hypersil ODS C18 reverse-phase column with 250 mm length, 5 μm diameter, and 4.6 mm width. The culture broth (100 μL) was sieved using a 0.22 μm filter. Mobile phase A of the HPLC was filled with 10.254 g of 99% sodium acetate three hydrates (Sigma-Aldrich, USA). It was then dissolved in 900 mL of deionized water and 500 μL trimethylamine (Merck). Glacial acetic was used to adjust the pH of the mobile phase A to 5.8. Acetonitrile (HPLC grade, Merck) and deionized water were the mobile phases B and C, respectively. Phases were filtered through 0.22 μm membrane filter, with an injection volume of 20 μL. The final compound was identified by the detector at 254 nm. The quantity of GABA produced was computed by comparing the peak to the GABA standard.
7
Poloxamer-188 Formulation for Diltiazem Delivery
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Poloxamer-188 was purchased from Merck (Darmstadt, Germany). Diltiazem hydrochloride (DLZ), stearyl alcohol (STA), and hydroxypropyl methylcellulose (HPMC) were obtained from Thermo Fisher Scientific (Loughborough, UK). Tildiem® (60 mg tablets) were ordered from Al Ain Pharmacy (Al Ain, UAE). Lecithin was acquired from Lipoid GmbH (Ludwigshafen, Germany). Sodium taurocholate (NaTC) and pepsin were obtained from Sigma Aldrich (St. Louis, MO, USA). Acetonitrile, 2−propanol, n-hexane, isopropyl alcohol, trimethylamine, ammonium dihydrogen phosphate, phosphoric acid, hydrochloric acid, glacial acetic acid, and sodium hydroxide were acquired from Merck (Darmstadt, Germany). All solvents used were of HPLC grade.
8
Lipid-Based Nanoparticle Formulation
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Epikuron®200 was purchased from ACEF (Fiorenzuola d’Arda, Italy), benzyl alcohol, propylene glycol, stearoyl chloride, cholesterol, butyl lactate from Fluka (Buchs, Switzerland), Fluorescein isothiocyanate, CS low viscosity, Sepharose®CL 4B, CURC, potassium chloride, Poly(ethylene glycol) 300, 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide, crystal violet solution from Sigma (Dorset, UK), Cremophor®RH60 from BASF (Ludwigshafen am Rhein, Germany), Taurocholic acid sodium salt from ICN Biomedicals (Solon, OH, USA), potassium phosphate monobasic, potassium phosphate dibasic from Merck (Darmstadt, Germany), methanol, trimethylamine, sodium chloride, acetic acid and ethanol from Carlo Erba (Val De Reuil, France). Deionized water was obtained by a MilliQ system (Millipore, Bedford, MO, USA).
9
Biomimetic 3D Scaffold Fabrication
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1,3-trimethylene carbonate (TMC) (Forusorb) was purchased from Huizhou Foryou Medical Devices (China) and used without any further purification. Stannous octoate (Sn(Oct)2), Dulbecco's modified Eagle's medium (DMEM), β-tricalcium phosphate (TCP), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), phalloidin-fluorescein isothiocyanate (FITC), gelatin and glutaraldehyde were purchased from Sigma (USA). 1,1,1tris(hydroxymethyl) propane, hydroquinone, methacrylic anhydride, trimethylamine, diphenyl(2,4,6trimethylbenzoyl)phosphine oxide (TPO-L), Orasol Orange-G, dichloromethane, ethanol, propylene carbonate, deuterated chloroform (CDCl3) and sodium borohydride were purchased from Merck (Germany). Recombinant human bone morphogenetic protein-2 (BMP) was purchased from Medtronics (USA), zoledronic acid (ZA) (Zoldonat) was purchased from Natco Pharma Ltd (India) and fetal bovine serum (FBS) was purchased from Gibco (USA). Penicillin-streptomycin antibiotic cocktail was purchased from Hi-Media (India), isoflurane (Forane) from Abbott (India), Ceftriaxone (Cefaxone) from Lupin (India), Tramadol (Dolphan) from GreenCross Remedies (India) and povidine-iodine (Betadine) from Win-Medicare (India). Nano-hydroxyapatite (HA) used was synthesized in the laboratory using the protocol as described elsewhere 51 (link) .
10
Radiolabeling of [18F]Fallypride Precursor
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