Graphite flakes (Bay Carbon), hydrogen peroxide (H
2O
2), sodium chloride (NaCl), potassium permanganate (KMnO
4), sodium nitrate (NaNO
3), and
potassium chloride (KCl) were purchased from Merck, Germany. Potassium phosphate monobasic (KH
2PO
4), sodium phosphate dibasic dihydrate (Na
2HPO
4), ethanolamine (HOCH
2CH
2NH
2), potassium hexacyanoferrate (II) trihydrate (K
4Fe(CN)
6·3H
2O), potassium hexacyanoferrate (III) (K
3Fe(CN)
6), N-(3-dimethyl aminopropyl)-N’-ethyl carbodiimide hydrochloride (EDC), sulfuric acid (H
2SO
4), and
N-hydroxysuccinimide (NHS) were procured from Sigma Aldrich, USA. Glass substrates with an ITO coating on one side (glass thickness: 1.1 mm; resistivity: 15–20 cm) were bought from Macwin in India. Tf antibody and anti-rabbit IgG peroxidase-labeled secondary antibody were procured from Thermo Scientific. Human holo-transferrin protein was procured from Sigma-Aldrich. All the reagents were synthesized in milli-Q water, with a resistivity of 18 M Ω cm.
Kaur H., Chittineedi P., Bellala R.S., Bellala V.M., Singh S., Kumari R., Chandra P., Pandrangi S.L, & Singh S.P. (2023). Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection of Transferrin in Serum Sample. Biosensors, 13(3), 406.