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84 protocols using graphite flakes

1

Characterization of Exfoliated Graphite

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Example 6

Additional analysis was performed to compare the parent material graphite flakes (Sigma-Aldrich) with exfoliated material produced from the graphite flakes in accordance with the techniques disclosed herein. The graphite flakes and exfoliated material were characterized by XRD spectroscope. FIG. 10 compares the XRD patterns of the graphite flakes (Sigma Aldrich) used in the Examples with exfoliated material produced in accordance with the techniques disclosed herein. The results indicate that the peak intensity decreased from 400,000 arbitrary units (a.u.) for graphite at 27.1° to 55,000 a.u. for the exfoliated material at 26.6°.

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2

Sustainable Graphite from Ground Nuts

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Ground nuts were taken from the local market of Muzaffarnagar (UP), India. Dry ground nut shells were utilized without any further treatment. All chemicals were used as received and included the following: graphite flakes (Merck), H2SO4 (Merck), KMnO4 (Merck), HNO3 (Merck), sodium hypochlorite (Himedia, India), aqueous 30 % (H2O2)(Rankem, India), NaOH (Rankem, India), HCl(Rankem, India), and phenol (LobaChemie, India)
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3

Synthesis of Graphene-based Composite Materials

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CoCl2·6H2O, graphite flakes, KMnO4, KOH, H2SO4 (98%), H3PO4 (85%), HCl (37%) and H2O2 (30%) were obtained from Merck chemical Co. Polytetrafluoroethylene (PTFE), N-methyl-2-pyrrolidone (NMP) and carbon black were purchased from Sigma-Aldrich Co. Ni foam was purchased from nano-BAZAR Co., Ltd. (Tehran, Iran). All chemicals were used without any further purification and all aqueous solutions were prepared using deionized water.
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4

Synthesis of Stimuli-Responsive Polymer Nanoparticles

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Graphite flakes, phosphoric acid (H3PO4), potassium permanganate (KMnO4), N,N-dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), copper bromide (CuBr), ε-caprolactone, N-isopropylacrylamide (NIPAm), and N-methylolacrylamide (NMA) were all purchased from Merck Chemical Co. Moreover, N-hydroxysuccinimide (NHS), N-ethylcarbodiimide hydrochloride (EDC), pyrrole, succinic anhydride, 3-bromo-1-propanol, stannous octoate (Sn(Oct)2), and N,N-dimethylformamide (DMF) were all purchased from Aldrich. All the chemicals were of analytical grade and used without any purification.
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5

Transferrin Antibody Immobilization Protocol

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Graphite flakes (Bay Carbon), hydrogen peroxide (H2O2), sodium chloride (NaCl), potassium permanganate (KMnO4), sodium nitrate (NaNO3), and potassium chloride (KCl) were purchased from Merck, Germany. Potassium phosphate monobasic (KH2PO4), sodium phosphate dibasic dihydrate (Na2HPO4), ethanolamine (HOCH2CH2NH2), potassium hexacyanoferrate (II) trihydrate (K4Fe(CN)6·3H2O), potassium hexacyanoferrate (III) (K3Fe(CN)6), N-(3-dimethyl aminopropyl)-N’-ethyl carbodiimide hydrochloride (EDC), sulfuric acid (H2SO4), and N-hydroxysuccinimide (NHS) were procured from Sigma Aldrich, USA. Glass substrates with an ITO coating on one side (glass thickness: 1.1 mm; resistivity: 15–20 cm) were bought from Macwin in India. Tf antibody and anti-rabbit IgG peroxidase-labeled secondary antibody were procured from Thermo Scientific. Human holo-transferrin protein was procured from Sigma-Aldrich. All the reagents were synthesized in milli-Q water, with a resistivity of 18 M Ω cm.
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6

Graphite Flakes Surface Modification

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Graphite flakes (GF), N-benzoyl-N-methylalanine, acetic anhydride, 4-aminothiophenol, silver nitrate, commercial nisin (~2.5% w/w) and solvents were purchased from Merck (Milan, Italy) and used as received.
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7

Enzymatic Dehalogenation of Chlorinated Solvents

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Thermomyces lanuginosus solution (Lipozyme® TL 100L) was from Novozymes (Copenhagen, Denmark). Copper (II) sulfate pentahydrate, sodium acetate and hydrogen peroxide (33%, H2O2) were from Panreac (Barcelona, Spain). Graphite flakes, sodium bicarbonate, sodium phosphate, sodium borohydride, Rhodamine B (RhB), trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE), and vinyl chloride (VC) were purchased from Merck (Darmstadt, Germany). HPLC grade acetonitrile, methanol, tetrahydrofurane (THF) and dioxane (98%) were from Scharlau (Barcelona, Spain). Tween-80, dodecyl sulphate sodium (85%), Mercaptoethanol and cetyl trimethyl ammonium bromide (CTAB) were purchased from Thermo Fischer Scientific (Waltham, MA, USA).
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8

Extraction and Cytotoxicity Evaluation of T. ornata

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Brown seaweed T. ornata was collected from the Rameswaram‐Mandapam, Ramnad District, and washed thoroughly with tap water to remove epiphytic growth and surface adhering salts. Then shade dried and pulverized. The pulverized seaweed was sieved with a 0.5 mm mesh and kept in a polyethylene bag at 4°C until use.
Methanol, graphite flakes, Sulphuric acid (H2SO4), nitric acid (HNO3), potassium permanganate (KMnO4), hydrogen peroxide solution (H2O2) were purchased from Merck (Mumbai). DMSO and MTT reagent were purchased from SRL Chemicals, India. RPMI‐1640 medium, Foetal bovine serum (FBS), penicillin, and streptomycin were purchased from Himedia India Pvt Ltd.
Cell lines were procured from NCCS, Pune, India, and the cytotoxicity assay was carried out at Lifeteck Research Centre, Chennai, India.
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9

Synthesis of Titanium-Samarium Nanocomposite

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Ti(OCH2CH2CH2CH3)4, Sm(NO3)3·6H2O, Na2MoO4·2H2O, Graphite flakes, H2SO4, KMnO4, H2O2, and HCl were
obtained from Merck chemicals.
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10

Graphene Oxide Synthesis and Characterization

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Graphene oxide
(GO) was synthesized using the modified Hummers method
followed by sonication.31 Briefly, graphite
flakes (Merck, USA), sodium nitrate, and potassium permanganate (SRL
Chemicals, Mumbai, India) were mixed at a ratio of 2:1:6 in the presence
of concentrated H2SO4 in an ice bath (at 0–5
°C). The reaction mixture was diluted by adding distilled water
under vigorous stirring followed by addition of hydrogen peroxide
(30%, v/v; Merck, India) to terminate the reaction. The resulting
mixture was centrifuged at ∼3000g for 10 min,
and the pellet was washed with 10% hydrochloric acid solution to remove
the metal ions followed by repeated washing with distilled water to
get the solution having pH 7. Purified GO was finely dispersed in
distilled water and sonicated for 2–4 h to facilitate the synthesis
of nanoparticulates.
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