Immunohistochemical and immunofluorescent staining of paraffin-embedded sections was performed by using a Discovery XT processor (Ventana Medical Systems). Tissue sections were deparaffinized with EZPrep buffer (Ventana Medical Systems) and subjected to antigen retrieval with CC1 buffer (Ventana Medical Systems). Sections were blocked for 30 minutes with Background Buster solution (Innovex) and for 8 minutes with avidin-biotin blocking (Ventana Medical Systems). Sections were incubated with anti-AR (Abcam, ab133273 1 μg/mL); ITGB4 (Cell Signaling, cat# 14803, 0.5 μg/mL); Vimentin (Cell Signaling, cat# 5741, 0.5 μg/mL); and synaptophysin (Abcam, ab32127, 1 μg/mL) antibodies for 5 hours followed by biotinylated horse anti-rabbit antibodies (Vector Labs, cat# PK6101) at 1:200 dilution (for AR, ITGB4, Vimentin) or HRP-conjugated goat anti-rabbit (PI-1000) antibodies at 1:250 dilution (for synaptophysin) for 60 minutes. Detection was performed with DAB detection kit (Ventana Medical Systems) according to manufacturer instruction. Slides were counterstained with hematoxylin and mounted using Permount (Fisher Scientific).
Itgb4
Manufactured by Vector Laboratories
ITGB4 is a product offered by Vector Laboratories. It is a laboratory equipment used for research purposes. The core function of ITGB4 is to detect and analyze the presence and distribution of the ITGB4 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated horse anti rabbit antibody, by Vector Laboratories (2 mentions)
Permount, by Thermo Fisher Scientific (2 mentions)
Dab detection kit, by Roche (2 mentions)
Cc1 buffer, by Roche (2 mentions)
Synaptophysin, by Vector Laboratories (2 mentions)
Discovery xt processor, by Roche (2 mentions)
Ezprep buffer, by Roche (2 mentions)
Avidin biotin blocking, by Roche (2 mentions)
Itgb4, by Cell Signaling Technology (2 mentions)
Vimentin, by Vector Laboratories (2 mentions)
2 protocols using itgb4
1
Immunohistochemical Staining of Paraffin Sections
2
Immunohistochemical Staining of Paraffin Sections
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Immunohistochemical and immunofluorescent staining of paraffin-embedded sections was performed by using a Discovery XT processor (Ventana Medical Systems). Tissue sections were deparaffinized with EZPrep buffer (Ventana Medical Systems) and subjected to antigen retrieval with CC1 buffer (Ventana Medical Systems). Sections were blocked for 30 minutes with Background Buster solution (Innovex) and for 8 minutes with avidin-biotin blocking (Ventana Medical Systems). Sections were incubated with anti-AR (Abcam, ab133273 1 μg/mL); ITGB4 (Cell Signaling, cat# 14803, 0.5 μg/mL); Vimentin (Cell Signaling, cat# 5741, 0.5 μg/mL); and synaptophysin (Abcam, ab32127, 1 μg/mL) antibodies for 5 hours followed by biotinylated horse anti-rabbit antibodies (Vector Labs, cat# PK6101) at 1:200 dilution (for AR, ITGB4, Vimentin) or HRP-conjugated goat anti-rabbit (PI-1000) antibodies at 1:250 dilution (for synaptophysin) for 60 minutes. Detection was performed with DAB detection kit (Ventana Medical Systems) according to manufacturer instruction. Slides were counterstained with hematoxylin and mounted using Permount (Fisher Scientific).
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