All chemicals and solvents were purchased from Merck (Germany) or Fluka (Switzerland). The fourier-transform infrared spectroscopy (FT-IR) spectra of the samples were recorded with the KBr pellet method by PerkinElmer PE-1600-FTIR spectrometer. A SIGMA VP 500 (Zeiss) microscope equipped with an EDX measurement system was used to record field emission scanning electron microscope (FESEM) imaging and energy-dispersive X-ray spectroscopy (EDX) analysis. Transmission electron microscopy (TEM) images were obtained using a SIGMA VP 500 (Zeiss) microscope. X-ray diffraction (XRD) spectra were carried out using an X-ray diffractometer (PANalytical X'Pert PRO, Netherlands) with Cu Kα radiation (λ = 1.54 Å). An ESCALab MKII (Thermo Fisher Scientific, USA) spectrometer with Al Kα (1.4866 keV) as the X-ray source was used to record X-ray photoelectron spectroscopy (XPS). Zeta potential was measured using a Zetasizer Nano-ZS, Model ZEN3600 (Malvern Instruments Ltd, Malvern, UK).
Sigma 500 vp
Manufactured by Zeiss
Sourced in Germany
The Sigma 500 VP is a high-precision laboratory equipment designed for a range of scientific applications. It features advanced optics and precise control mechanisms to ensure accurate and reliable measurements. The core function of the Sigma 500 VP is to provide researchers and scientists with a versatile and reliable tool for their analytical needs.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (4 mentions)
K alpha, by Thermo Fisher Scientific (4 mentions)
Pe 1600 ftir spectrometer, by PerkinElmer (4 mentions)
Drx 250 avance spectrometer, by Bruker (3 mentions)
Jem 2100, by JEOL (2 mentions)
Ultima 4, by Rigaku (2 mentions)
Oxford ultim max 40, by Oxford Instruments (2 mentions)
Lambda 750, by PerkinElmer (2 mentions)
D8 advance, by Bruker (2 mentions)
Lr white resin, by Electron Microscopy Sciences (2 mentions)
Uv 2100 151pc, by Shimadzu (2 mentions)
Escalab mk 2, by Thermo Fisher Scientific (2 mentions)
Agnps, by NanoComposix (1 mentions)
Ti spinning disk confocal microscope, by Nikon (1 mentions)
Spectramax m5, by Molecular Devices (1 mentions)
Prism 9, by GraphPad (1 mentions)
Spectrum 400 spectrometer, by PerkinElmer (1 mentions)
Vertex 70 system, by Bruker (1 mentions)
X ray diffractometer, by Malvern Panalytical (1 mentions)
Tristar 2 3020, by Micromeritics (1 mentions)
53 protocols using sigma 500 vp
1
Comprehensive Characterization of Nanomaterials
2
Quantifying Nanoparticle-Polysaccharide Interactions
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Unless otherwise noted, all reagents were purchased from commercial sources. Low–molecular weight TMC was purchased from Sigma-Aldrich (St. Louis, MO) with a reported >70% degree of quaternization. Forty kilodalton DS with 15 to 19% sulfur content was purchased from Sigma-Aldrich (St. Louis, MO). Forty-kilodalton FITC-labeled DS was purchased from TdB Labs (Uppsala, Sweden), with 16 to 19% reported sulfur content. All reagent solutions were freshly prepared for each experiment and fabrication process. AgNPs of 10 nm in diameter were purchased from nanoComposix (San Diego, CA). Glycerol stocks of PA14 were provided by O. Rosenberg. A549 lung cells were provided by the UCSF Cell and Genome Engineering Core.
Zeta potential and DLS measurements were conducted on a Malvern Zetasizer Nano ZS. Absorbance and fluorescence quantification measurements were conducted on a Molecular Devices SpectraMax M5 plate reader. All fluorescence microscopy studies were conducted at the UCSF Nikon Imaging Center using a Nikon Ti spinning disk confocal microscope. Scanning electron microscopy (SEM) images were obtained at the UCSF Bioengineering and Biomaterials Correlative Imaging Core using a Zeiss Sigma 500 VP (Carl Zeiss Microscopy GmbH). All calculations and statistical analyses were performed using GraphPad Prism 9.2 software.
Zeta potential and DLS measurements were conducted on a Malvern Zetasizer Nano ZS. Absorbance and fluorescence quantification measurements were conducted on a Molecular Devices SpectraMax M5 plate reader. All fluorescence microscopy studies were conducted at the UCSF Nikon Imaging Center using a Nikon Ti spinning disk confocal microscope. Scanning electron microscopy (SEM) images were obtained at the UCSF Bioengineering and Biomaterials Correlative Imaging Core using a Zeiss Sigma 500 VP (Carl Zeiss Microscopy GmbH). All calculations and statistical analyses were performed using GraphPad Prism 9.2 software.
3
Hybrid Lipid-Hydrogel Formulation for Topical Delivery
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FC+ prepared by a gel-phase dispersion process of lipidated CAAS into FG-hydrogel matrix to form a hybrid-hydrogel (hybrid-FENUMAT™). Briefly, about 4.2 g of CAAS was dissolved in water, mixed with an ethanolic solution of lecithin (2.1 g), and homogenized. The homogeneous solution was then mixed uniformly with fenugreek galactomannan solution and the resulting mass was evaporated to dry powder form.
The physicochemical properties including particle size, zeta potential, Fourier-transform infrared spectroscopy (FTIR) and morphology of the formulation FC+ was carried out using high resolution transmission electron microscope (HR-TEM) (JEOL JEM-2100 LaB6, Jeol Co Limited, Japan), field emission scanning electron microscope (FE-SEM) (ZEISS Sigma 500 VP, ZEISS microscopy, Oberkochen, Germany), dynamic light scattering (DLS) (Horiba SZ-100 particle size analyser, Horiba India Private Limited, Bengaluru, India) and FTIR spectra from PerkinElmer Spectrum 400 spectrometer.
The physicochemical properties including particle size, zeta potential, Fourier-transform infrared spectroscopy (FTIR) and morphology of the formulation FC+ was carried out using high resolution transmission electron microscope (HR-TEM) (JEOL JEM-2100 LaB6, Jeol Co Limited, Japan), field emission scanning electron microscope (FE-SEM) (ZEISS Sigma 500 VP, ZEISS microscopy, Oberkochen, Germany), dynamic light scattering (DLS) (Horiba SZ-100 particle size analyser, Horiba India Private Limited, Bengaluru, India) and FTIR spectra from PerkinElmer Spectrum 400 spectrometer.
4
Ruthenium Surface Characterization Protocol
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Before immersion in the solution to be tested, the sample processing method was as follows: 1 × 1 cm samples were cut from a 12 inch ruthenium blanket wafer and soaked in a 25 mM citric acid solution for 10 minutes, followed by rinsing with deionized water and finally dried with pressure-air. The morphological characteristics of the ruthenium soaked in the test solutions for 15 minutes was characterized by atomic force microscopy (AFM; 5600LS, Agilent) and scanning electron microscopy (SEM; Zeiss Sigma 500/VP, Carl Zeiss). The X-ray photoelectron spectroscopy (XPS) device used herein was manufactured by Thermo Scientific and was model number ESCALAB250Xi. After testing, the XPS spectra was analyzed by CasaXPS software and calibrated according to the C1s peak (284.6 eV).
5
Microstructural Analysis of Cured Composites
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Microstructural analysis was conducted on the GPC samples to examine the effect of varying curing temperature for a constant CF content. This analysis aimed to elucidate the reasons behind the observed enhancement in mechanical properties, concurrent with a decrease in volume density and alterations in total porosity. Samples containing 37.5% of CFs and cured in various temperatures were investigated (samples: 7, 8, 9).
The microstructure of mortars was analyzed using the SEM model Sigma 500 VP produced by ZEISS (Carl Zeiss Microscopy GmbH, Köln, Germany). BSE (backscattered electron) images were collected. Microanalysis was performed using EDX (energy-dispersive X-ray spectroscopy) detector model Ulitim Max 40 produced by Oxford (Oxford Instruments, High Wycombe, UK). For SEM analysis, thin slices were cut from the middle of each type of mortar bar perpendicular to the trowelling surface. The slices were trimmed to achieve surfaces measuring 20 × 20 mm in dimensions. Samples were dried and put into resin under vacuum. The next step was grinding and polishing samples to receive the proper surface for SEM-EDX examinations. The procedure of sample preparation is described widely in previous publications [32 (link)]. Before SEM examinations, the samples were gold evaporated.
The microstructure of mortars was analyzed using the SEM model Sigma 500 VP produced by ZEISS (Carl Zeiss Microscopy GmbH, Köln, Germany). BSE (backscattered electron) images were collected. Microanalysis was performed using EDX (energy-dispersive X-ray spectroscopy) detector model Ulitim Max 40 produced by Oxford (Oxford Instruments, High Wycombe, UK). For SEM analysis, thin slices were cut from the middle of each type of mortar bar perpendicular to the trowelling surface. The slices were trimmed to achieve surfaces measuring 20 × 20 mm in dimensions. Samples were dried and put into resin under vacuum. The next step was grinding and polishing samples to receive the proper surface for SEM-EDX examinations. The procedure of sample preparation is described widely in previous publications [32 (link)]. Before SEM examinations, the samples were gold evaporated.
6
Morphology Analysis of Prepared Mixtures
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The morphology of the prepared mixtures as well as the pure ingredients, was evaluated using a Sigma 500 VP scanning electron microscope (Zeiss, Jena, Germany). To improve the discharge process, just before measurements, the investigated samples were covered with a layer of gold using a Quorum machine (Quorum International, Fort Worth, TX, USA).
7
Scanning Electron Microscopy of E. coli Exposed to BCp12
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E. coli suspensions (about 106 CFU/mL) were prepared and separately treated with 0.5× and 1× MIC BCp12. All samples were incubated at 37 °C for 12 h, and the suspensions were centrifuged at 10,000 rpm, 4 °C for 5 min. The precipitated cells were washed thrice and resuspended with PBS. The bacterial precipitate was fixed with 2.5% glutaraldehyde for 6 h. The fixing solution was subsequently removed via centrifugation. The precipitate was again washed thrice with PBS and dehydrated with ethanol standard solutions in a concentration gradient (30%, 50%, 70%, 90%, and 100%) for 10 min (with each ethanol solution in order of increasing concentration). The precipitate was then dried at 60 °C for sputter coating. Cells treated as described above and not exposed to BCp12 were considered as negative controls. A scanning electron microscope (SIGMA 500/VP, Carl Zeiss, Germany) was used for visualization [28 (link)].
8
HRSEM and EDX Analysis of Foams
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High-resolution scanning electron microscopy (HRSEM) utilizing a ZEISS Sigma 500 VP instrument was used to study the morphology of the foams. Energy dispersive X-ray (EDX) measurements were achieved in parallel by employing the EDX system included in the electron microscope.
9
Characterization of Synthesized Materials
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The crystalline structure of the synthesized materials was analyzed by X-ray diffraction (XRD) using an X-ray diffractometer (PANalytical, Empyrean, Netherlands) equipped with Cu Kα radiation of wavelength 1.54045 Å at an operating voltage of 40 kV with an operational current of 30 mA. Data were obtained using monochromatic Al K radiation from 10 to 1350 eV at a pressure of 109 mbar at a full-spectrum pass energy of 200 eV and a narrow spectrum of 50 eV for the X-ray photoelectron spectroscopy (XPS) analysis (K-ALPHA, Thermo Fisher Scientific, USA). The Fourier transform infrared (FTIR) spectra of the samples were recorded using a Vertex 70 system (Bruker, Germany) in the wavenumber region of 400–4000 cm−1 to predict the presence of the different functional groups. The surface morphologies of the samples were characterized by scanning electron microscopy (SEM, ZEISS, Sigma 500 VP). The microstructures and morphologies of the samples were characterized by high-resolution transmission electron microscopy (HR-TEM, JEM2100, Jeol, Japan), operated at an acceleration voltage of 200 kV. The textural characteristics, including the surface areas and pore-size distributions of the sample, were investigated using N2 adsorption–desorption isotherms, tested with a Tri-Star II 3020 (Micromeritics, USA) analyzer by applying the Brunauer–Emmett–Teller (BET) method.
10
Concrete Self-Healing Evaluation
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The removal of the specimens was taken place after 24 h casting and then cured with water. Standard molds of Beam for mortar specimens (40 × 40 × 160 mm) were used. Compression and self-healing were conducted at various curing times of 7, 14, and 28 days for hardened concrete. The compressive, flexural, and indirect tests of splitting tensile strength for hardened concrete after 28 days were conducted for concrete specimens. Cylinder molds of 100 × 200 mm, 100 mm cubic, 100 × 100 × 500 mm beam were used. Besides, to track microstructural changes due to calcite formation, samples were also subjected to a SEM. Bacterial calcite precipitation was scanned by using SEM micrographs in micro-cracks specimens. These micrographs were created using a Carl Zeiss sigma 500 VP. A stereomicroscope was used for mortar self-healing measurements.
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