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Tlr2 cb225

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The TLR2 (CB225) is a laboratory equipment product manufactured by BD. It is a tool used for the detection and analysis of the Toll-like receptor 2 (TLR2) protein, which plays a crucial role in the immune system's innate response. The TLR2 (CB225) provides researchers and scientists with a reliable and efficient way to study the expression and function of TLR2 in various experimental settings.

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Lab products found in correlation

Cytofix solution, by BD (2 mentions) Cd11a m17 4, by Thermo Fisher Scientific (2 mentions) Tlr4 sa15 21, by BioLegend (2 mentions) Cd3ε 145 2c11, by BioLegend (2 mentions) Nk1.1 pk136, by Thermo Fisher Scientific (2 mentions) Nkp46 29a1.4, by BioLegend (2 mentions) F4 80 bm8, by BioLegend (2 mentions) Cd19 6d5, by BioLegend (2 mentions) Cd11c hl3, by BD (2 mentions) Ly6g 1a8, by BioLegend (2 mentions) Cd127 a7r34, by BioLegend (2 mentions) Ly6c hk1, by BioLegend (2 mentions) Cd8α 53 6.7, by BioLegend (2 mentions) Cd4 gk1, by BioLegend (2 mentions)

2 protocols using tlr2 cb225

1

Multiparametric Immune Cell Profiling

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Peripheral blood was collected retro-orbitally from isoflurane-anesthetized mice. The blood was treated with ACK lysis buffer for 5 min to remove RBCs, leaving the peripheral blood leukocytes (PBLs). To determine expression of cell surface molecules, we incubated PBLs with mAb at 4°C for 20–30 min, and cells were subsequently fixed for 10 min using Cytofix Solution (BD Biosciences). The following mAb clones were used to stain processed samples: CD11a (M17/4; eBioscience), TLR4 (SA15-21; BioLegend), CD3ε (145-2C11; BioLegend), NK1.1 (PK136; eBioscience), NKp46 (29A1.4; BioLegend), F4/80 (BM8; BioLegend), TLR2 (CB225; BD Bioscience), CD19 (6D5; BioLegend), CD11c (HL3; BD Biosciences), CD4 (GK1.5; BioLegend), Ly6G (1A8; BioLegend), CD127 (A7R34; BioLegend), Ly6C (HK1.4; BioLegend), and CD8α (53-6.7; BioLegend). Flow cytometry data were acquired on a Cytek Aurora (Cytek, Bethesda, MD) and analyzed with FlowJo software (Tree Star, Ashland, OR).
Berton R.R., Jensen I.J., Harty J.T., Griffith T.S, & Badovinac V.P. (2022). Inflammation Controls Susceptibility of Immune-Experienced Mice to Sepsis. ImmunoHorizons, 6(7), 528-542.
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2

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood was collected retro-orbitally from isoflurane-anesthetized mice. The blood was treated with ACK lysis buffer for 5 min to remove RBCs, leaving the peripheral blood leukocytes (PBLs). To determine expression of cell surface molecules, we incubated PBLs with mAb at 4°C for 20–30 min, and cells were subsequently fixed for 10 min using Cytofix Solution (BD Biosciences). The following mAb clones were used to stain processed samples: CD11a (M17/4; eBioscience), TLR4 (SA15-21; BioLegend), CD3ε (145-2C11; BioLegend), NK1.1 (PK136; eBioscience), NKp46 (29A1.4; BioLegend), F4/80 (BM8; BioLegend), TLR2 (CB225; BD Bioscience), CD19 (6D5; BioLegend), CD11c (HL3; BD Biosciences), CD4 (GK1.5; BioLegend), Ly6G (1A8; BioLegend), CD127 (A7R34; BioLegend), Ly6C (HK1.4; BioLegend), and CD8α (53-6.7; BioLegend). Flow cytometry data were acquired on a Cytek Aurora (Cytek, Bethesda, MD) and analyzed with FlowJo software (Tree Star, Ashland, OR).
Berton R.R., Jensen I.J., Harty J.T., Griffith T.S, & Badovinac V.P. (2022). Inflammation Controls Susceptibility of Immune-Experienced Mice to Sepsis. ImmunoHorizons, 6(7), 528-542.
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