Celeris rodent erg system

Dynamic light scattering (DLS) analysis was conducted by Nano‐ZS (Malvern Instruments, UK). Transmission electron microscopy (TEM) images were captured by CM100 Transmission Electron Microscope (Philips, USA). UV‐Vis absorption spectra and cell viability were measured by SpectraMax M4 multi‐mode microplate reader (Molecular Devices, USA). Drug‐loading capacity, encapsulation efficiency, and drug concentrations were measured by 1260 Infinity II HPLC (Agilent Technologies, USA). Photolysis experiments and irradiation were conducted using a commercial diode laser system (Laserwave, Canada). The light irradiance was measured by PM100USB power and energy meter (Thorlabs, USA) equipped with S142C integrating sphere photodiode power sensor (Si, 350–1100 nm, Thorlabs, USA). Fluorescence images were captured by LSM 980 confocal microscope (ZEISS, German). Intraocular DAS content was detected by API 4000 liquid chromatograph‐triple quadrupole mass spectrometry (AB Sciex, USA). Experimental CNV mouse model was constructed using OcuLight Infrared Laser System (IRIDEX, USA). Anterior chamber and fundus images were acquired by SL‐D701/BG‐5/IMAGEnet digital slit lamp photography system (Topcon, Japan) and Micron IV retina imaging system (Phoenix Research Laboratories, USA), respectively. Electroretinography (ERG) tests were examined by Celeris rodent ERG system (Diagnosys LLC, USA).

Full field ERGs were recorded in dark-adapted (18 h) mice (Rpe65-450Met, agouti) at the indicated ages. The mice were anaesthetized with an intraperitoneal injection of a mixture of ketamine (80 mg/kg), xylazine (10 mg/kg), and acepromazine (5 mg/kg). Pupils were dilated with topical phenylephrine hydrochloride and tropicamide and body temperature was maintained at 35 to 37 °C. Recordings were acquired with a Celeris rodent ERG system (Diagnosys) and a pair of corneal electrodes positioned with GenTeal Tears gel (Alcon Laboratories). Scotopic ERG responses in dark-adapted mice were elicited by single flashes having intensities from 0.01 cd·s/m² to 100 cd·s/m². Photopic responses from 0.1 to 100 cd⋅s/m² intensities were recorded in mice adapted to background light of 30 cd⋅s/m² (10 min). Triplicate responses were computer-averaged for each flash condition. The amplitude of the a-wave was measured from the baseline to the first trough. The amplitude of the b-wave was measured from the trough of the a-wave to the following positive peak.