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2 protocols using 1 1 diphenyl 2 picrylhydrazyl dpph

1

Antioxidant and Antibacterial Evaluation

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Methanol, hexane, chloroform, ethyl acetate and ethanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. Potassium phosphate monobasic and dibasic, xanthine, xanthine oxidase, allopurinol, and hydrochloric acid were obtained from Sigma-Aldrich Corp., St. Louis, MO, USA. Reagents including 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium acetate, acetic acid, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium peroxodisulfate, and dibutyl hydroxytoluene (BHT) were supplied by Kanto Chemical Co. Inc., Tokyo, Japan. Four bacteria including Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Proteus mirabilis were provided by Sigma-Aldrich Corp., St. Louis, MO USA. All chemicals used were of analytical grade.
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2

Antioxidant and Antimicrobial Assays

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Folin-Ciocalteu’s phenol, potassium phosphate monobasic and dibasic, xanthine, xanthine oxidase, allopurinol, and hydrochloric acid were obtained from Sigma-Aldrich Japan K.K., Tokyo, Japan. Aluminium (III) chloride hexahydrate, 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium acetate, acetic acid, dibutyl hydroxuytoluene (BHT), gallic acid, and quercetin were purchased from Kanto Chemical Co. Inc., Tokyo, Japan. Acetone, potassium peroxodisulfate, 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were obtained from Nacalai Tesque, Inc., Kyoto, Japan. Methanol (MeOH), ethyl acetate (EtOAc), ethanol, and methanol plus were obtained from Junsei Chemical Co., Ltd., Tokyo, Japan. All of the bacteria strains, including Escherichia coli, Proteus mirabilis, Staphylococcus aureus, and Bacillus subtilis, were purchased from Sigma-Aldrich (Tokyo, Japan).
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