7200 accurate mass q tof mass spectrometer

The quantification was based on a modified method of the fast GC method reported by Tsochatzis et al. [1 (link)] using styrene and PS oligomer standards, which were prepared in CHCl₃, and external calibration was applied, according to a previously reported method [1 (link)]. The organic CHCl₃ extracts were filtered with PTFE 0.22 μm filters, and a volume of 1 μL was injected by the GC-TOF-MS system in split-less mode into an Agilent Technologies 7890B gas chromatography system coupled to an Agilent Technologies 7200 Accurate-Mass Q-ToF mass Spectrometer (Agilent Technologies, Waldbronn, Germany). Separation of the metabolites was performed on an HP-5MS capillary column (30 m, 0.250 mm i.d., 0.25 μm film thickness, Agilent Technologies). The ion source temperature was set to 230 °C, and the MS analysis was performed in scan mode with a quadruple temperature of 150 °C and a fragmentation voltage of 70 eV (pressure of 21.4 psi, a gas saver of 20 mL/min and 3 mL/min purge flow). The GC oven temperature program followed the temperature program previously reported in [1 (link)]. Briefly, an isotherm of 60 °C was held for 2 min, followed by a temperature ramp of 10 °C/min up to a temperature of 320 °C that was held for 5 min. The total analysis time was 34 min.