Mouse cerebellum tissue was lysed using a bath-type sonicator (Cosmobio, Bioruptor UCD-250) in PBS buffer. Tissue and cell debris were removed by centrifugation at 13,200 rpm at 4°C for 10 min. After centrifugation, supernatant was separated into hydrophobic and hydrophilic phases using ice-cold chloroform and methanol (2:1, v/v) according to the method of Bligh and Dyer (46 (link)). These phases were dried by centrifugation under vacuum at 45°C for 4 hours.
Ucd 250
Manufactured by Cosmo Bio
Sourced in Japan
The UCD-250 is a centrifuge that can separate substances of different densities in a liquid mixture. It has a maximum speed of 25,000 RPM and can accommodate sample volumes up to 250 milliliters. The UCD-250 is designed for general laboratory use.
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Lab products found in correlation
2 protocols using ucd 250
1
Cerebellum Tissue Lipid Extraction
2
E. coli Cell Lysis and Enzyme Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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E. coli cells were grown in LB medium containing appropriate antibiotics to the mid-log phase (A660 ≅ 0.6), collected through centrifugation (15,000× g, 4 °C, 5 min), washed thrice with ice-cold 50 mM potassium phosphate buffer (pH 8.0) or 50 mM Tris-HCl (pH 8.0), and then resuspended in the original volume of the same solution. The collected cells were disrupted by applying 5 cycles of 15-s sonic oscillation with 45-s intermittent cooling in an ice bath by using a Bioruptor (UCD-250, CosmoBio Co., Tokyo, Japan). The supernatant obtained through centrifugation (15,000× g, 4 °C, 15 min) was used for assaying enzyme activity.
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