Anti cd3 cd28 coated beads

Manufactured by BD

Anti-CD3/CD28 coated beads are a type of lab equipment used for T-cell activation and expansion. The beads are coated with antibodies that bind to the CD3 and CD28 surface receptors on T-cells, triggering their activation and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Legendplex human th2 panel, by BioLegend (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Cfse labeled, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Irvine Scientific (1 mentions) Penicillin, by Irvine Scientific (1 mentions) Glutamine, by Irvine Scientific (1 mentions) Human serum, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Fcs express 6, by De Novo Software (1 mentions)

Close spelling variants of this product

Anti-CD3/CD28 beads CD3/CD28 beads Anti-CD3/CD28 Coated anti-CD3 Anti-CD28 Anti-CD3

3 protocols using anti cd3 cd28 coated beads

T cell Proliferation Assay with MDSC Suppression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Effector CBMC or CD3^pos T cells were CFSE-labeled (Molecular Probes) and cultured at 2×10⁶ cells/ml. T cells were stimulated with anti-CD3/CD28 coated beads (BD) in RPMI plus 15% human sera with the daily addition of 0.1 ng/mL rhuIL-7 (R&D Systems). CD15^pos MDSC were depleted from CBMC or added to purified T cell cultures at a 1∶1 effector-to-suppressor ratio. Negative controls included effector cells with or without suppressor cells, without anti-CD3/CD28 stimulation. On day 5, cells were permeabilized, stained with anti-CD3 (UCHT1 or SK7), CD4 (RPA-T4), CD8 (SK1) from BD Biosciences and proliferation of T cells was assessed by analysis of lymphoid-gated, CD3^pos, CFSE^lo cell populations. Positive proliferative responses were calculated after subtraction of background proliferation from corresponding negative control wells. Percent suppression was calculated by the following formula: %CFSE^lo (CBMC) - %CFSE^lo (CBMC-CD15)/%CFSE^lo (CBMC-CD15) ×100.

Gervassi A., Lejarcegui N., Dross S., Jacobson A., Itaya G., Kidzeru E., Gantt S., Jaspan H, & Horton H. (2014). Myeloid Derived Suppressor Cells Are Present at High Frequency in Neonates and Suppress In Vitro T Cell Responses. PLoS ONE, 9(9), e107816.

+ Open protocol

+ Expand

T Cell Subsets Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorted T cell subsets were incubated at 5000 cells per well in 96-well plates for 48 hours with 2 μl per well anti-CD3/CD28 coated beads (BD Biosciences), 10% human serum (Gemini), HEPES (Gibco BRL), glutamine, penicillin, streptomycin (Irvine Scientific), and 2-mercaptoethanol (MilliporeSigma) in RPMI (Invitrogen). Different plates were set up for different time points. After 48 hours of culture, 100 μl culture supernatant was collected and concentrations of IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, and TNF-α were determined by Flow Cytometry and the Biolegend LegendPlex Human Th2 panel.

Narsale A., Lam B., Moya R., Lu T., Mandelli A., Gotuzzo I., Pessina B., Giamporcaro G., Geoffrey R., Buchanan K., Harris M., Bergot A.S., Thomas R., Hessner M.J., Battaglia M., Serti E, & Davies J.D. (2021). CD4+CD25+CD127hi cell frequency predicts disease progression in type 1 diabetes. JCI Insight, 6(2), e136114.

+ Open protocol

+ Expand

CFSE-based T cell proliferation assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorted CD4⁺ T cell subsets were labeled with CFSE
(Molecular Probes) using the standard published protocol [29 (link)] before culture, and incubated
in duplicate at 10⁵ cells per well in 48 well plates for 48 and 72
hours with 10 μl per well anti-CD3/CD28 coated beads (BD Biosciences),
10% human serum (Gemini), HEPES (Gibco BRL), glutamine, penicillin,
streptomycin (Irvine Scientific), and 2-mercaptoethanol (Sigma-Aldrich) in RPMI
(Invitrogen). Different plates were set up for different time points. For
cytokine analysis, 100 μl of culture supernatant was collected at 48
hours and concentrations of IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ
and TNF-α were determined by Flow Cytometry using the Cytometric Bead
Array following the manufacturer’s instructions (BD Biosciences Human
Th1/Th2/Th17 CBA kit and Biolegend LegendPlex Human Th2 panel). For cell
proliferation, cells were washed twice after 72 hours of culture and CFSE
dilution determined by Flow Cytometry. FCS Express 6 from De Novo Software
(Glendale, CA) was used to calculate proliferation index and percent divided
cells.

Narsale A., Moya R, & Davies J.D. (2018). Human CD4+ CD25+ CD127hi cells and the Th1/Th2 phenotype. Clinical immunology (Orlando, Fla.), 188, 103-112.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!