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Ercc1

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ERCC1 is a protein that is involved in the nucleotide excision repair pathway, which is responsible for repairing DNA damage. This protein plays a key role in the recognition and removal of DNA lesions caused by various environmental factors, such as UV radiation and certain chemotherapeutic agents.

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Lab products found in correlation

Sparc, by R&D Systems (1 mentions) Pd l1, by BD (1 mentions) Pdgfra, by Thermo Fisher Scientific (1 mentions) Benchmark xt, by Roche (1 mentions) Autostainer link 48, by Agilent Technologies (1 mentions) Tubb3, by Fortrea (1 mentions) Cisplatin, by Selleck Chemicals (1 mentions) Bcl 2, by Abcam (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) Mmp 2 9, by Cell Signaling Technology (1 mentions) Hoxb9, by Thermo Fisher Scientific (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) β tubulin, by Abcam (1 mentions)

Spelling variants of this product

Anti-ERCC1 (3 citations) ERCC1 (8F1) (3 citations)

4 protocols using ercc1

1

Comprehensive Immunohistochemical Protein Analysis

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Protein expression was assessed by immunohistochemistry (IHC), as previously described.19 (link) Cancer cells on slides were scored by pathologists. For PD-1, the tumor-infiltrating lymphocytes (TILs) were scored. Protein was considered overexpressed, when the percent staining and intensity were above previously published and validated thresholds specific to each marker, for proteins where increased expression levels were of interest; when loss of protein or underexpression of protein was of interest, percent staining and intensity below previously published and validated levels were reported.19 (link) Antibodies used included: androgen receptor (AR), topoisomerases 1 and 2 (TOPO1, TOPO2A, Leica Biosystems), estrogen receptor (ER), progesterone receptor (PR), cMet, human epidermal growth factor receptor 2 (HER2; Ventana), cKIT, epidermal growth factor receptor (EGFR), phosophatase and tensin homolog (PTEN; Dako), O(6)-methylguanine-methyltransferase (MGMT), PGP, thymidylate synthase (TS; Invitrogen), transducin-like enhancer of split 3 (Santa Cruz), excision repair cross complimentary group 1 (ERCC1; Abcam), RRM1 (Proteintech), SPARC (monoclonal; R&D Systems and polyclonal; Exalpha) and tubulin beta-3 chain (Covance), programmed cell death protein 1 (PD-1), and programmed death-ligand 1 (PD-L1; BD Pharmingen and R&D Systems).
Millis S.Z., Ejadi S, & Demeure M.J. (2015). Molecular Profiling of Refractory Adrenocortical Cancers and Predictive Biomarkers to Therapy. Biomarkers in Cancer, 7, 69-76.
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2

Comprehensive Immunohistochemical Protein Profiling in Sarcoma

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Protein expression was determined by IHC analysis, using commercially available detection kits and automated staining techniques (Benchmark XT, Ventana, and AutostainerLink 48, Dako) [53 (link)]. Antibodies used included: androgen receptor (AR), topoisomerases 1 and 2 (TOPO1, TOPO2A) (Leica Biosystems); ER, PR, cMET, HER2 (Ventana); cKIT, epidermal growth factor receptor (EGFR), phosophatase and tensin homolog (PTEN) (Dako), O(6)-methylguanine-methyltransferase (MGMT), P-glycoprotein (PGP) (Invitrogen); transducin-like enhancer of split 3 (TLE3, Santa Cruz); ribonucleotide reductase M1 (RRM1, Protein Tech); SPARC (monoclonal, R&D Systems; polyclonal, Exalpha), tubulin beta-3 chain (TUBB3) (Covance), Excision Repair Cross-Complementation Group 1 (ERCC1, (Abcam), platelet derived growth factor receptor alpha (PDGFRA, Thermo), Programmed cell death protein 1 (PD-1) and Programmed death-ligand 1 (PD-L1) (BD Pharmingen and R&D Systems). IHC thresholds previously validated in other cancers were used, as cutoffs are not established in sarcoma (Appendix 1).
Movva S., Wen W., Chen W., Millis S.Z., Gatalica Z., Reddy S., von Mehren M, & Van Tine B.A. (2015). Multi-platform profiling of over 2000 sarcomas: Identification of biomarkers and novel therapeutic targets. Oncotarget, 6(14), 12234-12247.
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3

Western Blot Analysis of Apoptotic Markers

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The following antibodies were purchased (Supplementary Table S1): primary antibodies to homeobox B9 (HOXB9, Thermo Fisher Scientific, Waltham, MA, USA), matrix metalloproteinase-2, -9 (MMP-2, -9, Cell Signaling Technology, Danvers, MA, USA), B-cell lymphoma 2 (BCL-2, abCAM, Cambridge, UK), BCL2-associated X (BAX, abCAM, Cambridge, UK), E-Cadherin, Survivin, Caspase-3, Cleaved Caspase-3, Cleaved Caspase-9, PARP, Cleaved PARP (Cell Signaling Technology, Danvers, MA, USA), excision repair cross-complementation-1 (ERCC-1, abCAM, Cambridge, UK), multidrug resistance-associated protein 2 (MRP-2, abCAM, Cambridge, UK), X-linked inhibitor of apoptosis protein (XIAP, Cell Signaling Technology, Danvers, MA, USA) and α-tubulin (Cell Signaling Technology, Danvers, MA, USA). The anti-rabbit IgG and anti-mouse IgG HRP (Horseradish Peroxidase)-conjugated secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Cisplatin was purchased from Selleckchem (Houston, TX, USA).
Suh D.H., Park W.H., Kim M., Kim K., No J.H, & Kim Y.B. (2023). HOXB9 Overexpression Confers Chemoresistance to Ovarian Cancer Cells by Inducing ERCC-1, MRP-2, and XIAP. International Journal of Molecular Sciences, 24(2), 1249.
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4

Immunoblot Analysis of Cellular Proteins

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Cells were lysed on ice for 30 min using RIPA buffer and PMSF (Beyotime, Shanghai, China). Proteins were separated by SDS-PAGE and transferred to a Polyvinylidene Fluoride membrane (Millipore, Bedford, MA). After blocking with 5% dry milk in TBST for 2 h at room temperature, the membranes were incubated with the primary antibodies against human SDHB (1:1000, Epitomics), β-tubulin (1:4000, Epitomics), βactin (1:500, Abmart), ERCC1 (1:400, Abcam), phospho-histone H2A.X (γ-H2A.X, 1:1000, Cell Signaling Technology) in dilution buffer overnight at 4°C. After washing three times with TBST, memmbranes were incubated with IRDye 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG or Anti-Mouse IgG (1:10000) antibodies for 1 h at room temperature. The expression of speci c proteins was detected through the use of Odyssey system following the manufacturer's instructions.
Chen L., & Di W. (2022). Succinate Dehydrogenase Subunit B Regulates Cisplatin Resistance via DNA Damage in Human Ovarian Cancer Cells.
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