FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
Pe conjugated cd11b
Manufactured by Thermo Fisher Scientific
Sourced in United States
PE-conjugated CD11b is a fluorescently labeled antibody that binds to the CD11b surface antigen. CD11b is a cell surface receptor expressed on various immune cells, including monocytes, macrophages, and granulocytes. The PE fluorescent dye is used to label the CD11b antibody, enabling its detection and quantification through flow cytometry or other fluorescence-based analysis methods.
Automatically generated - may contain errors
Lab products found in correlation
Fitc and pe conjugated anti mouse cd8a clone 53, by BD (2 mentions)
Fitc conjugated anti mouse ifn γ clone xmg1, by BD (2 mentions)
Purified anti mouse cd16 32 fc block, by BD (2 mentions)
Apc conjugated cd25, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated gr 1, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated anti mouse cd4, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated cd71, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Moflo machine, by Beckman Coulter (1 mentions)
Lsrii flow cytometer, by FlowJo (1 mentions)
Perm wash buffer, by BD (1 mentions)
Calibur, by BD (1 mentions)
Cellquest pro, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
6 protocols using pe conjugated cd11b
1
Multicolor Flow Cytometry Analysis
2
Immunophenotyping of Hematopoietic Lineages
3
Multi-parameter Flow Cytometry Analysis
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All antibodies were purchased from BD PharMingen or eBiosciences (CA, USA) as follows: PE-conjugated CD11b, APC-conjugated Annexin V, and APC-conjugated Ki67. Total cells were Fc-blocked and stained with indicated combinations of antibodies for 30 min on ice, then washed three times and resuspended in 1% FBS/PBS. For apoptosis analysis, cells were resuspended with binding buffer and stained with Annexin V and 7-AAD for 15 min at 25 °C in the dark. For cell cycle analysis, cells were thoroughly suspended and incubated with fixation and permeabilization solution for 20 min at room temperature, washed twice with BD Perm/Wash buffer, and stained with KI-67 and HO33342 for 30 min. The flow cytometric data were collected on a BD Calibur or a LSRII flow cytometer and analyzed using FlowJo software or Summit software. For the fluorescence-activated cell sorting (FACS), the nucleated cells were stained with the indicated antibodies and resuspended in 2% FBS/PBS. The cells were sorted using a MoFlo machine (Beckman Coulter).
4
Multicolor Flow Cytometry Analysis
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FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
5
Flow Cytometric Analysis of MSC Surface Markers
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For surface marker analysis, the MSCs were detached and stained with anti-mouse monoclonal antibodies for 30 min at 4°C, including PE-conjugated CD11b (cat no. 12-0112-82; eBioscience, San Diego, CA, USA), CD44 (cat no. 553134), CD45 (cat no. 553081); FITC-conjugated CD29 (cat no. 561796), and CD34 (cat no. 560238; BD Pharmingen, San Diego, CA, USA). The labeled cells were analyzed using flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA). PE-IgG and FITC-IgG were used as controls; for all antibodies, 1 µl antibody was added to 2.5×105 cell suspension in 200 µl PBS. The analysis of the DNA content of the MSCs was performed as described above. The cell pellets were incubated in propidium iodide on ice for 30 min. The percentages of cells in the G0/G1, G2/M and S phases were analyzed using flow cytometry using BD CellQuest™ Pro software (BD Biosciences).
6
Neutrophil Differentiation Assay
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The Lin− cells were collected by BD Influx and cultured with 100 ng/ml rmG-CSF for 10 days with PHPS-1 treatment (20 μM). Neutrophils were analyzed on culture day 10. For analysis of neutrophils, cells were stained with APC-eFluor780-conjugated Gr-1 (eBioscience, San Diego, CA, USA) and PE-conjugated CD11b (eBioscience) in the presence of anti-CD16/32 block (2.4 G2; BD Pharmingen, San Jose, CA, USA). Gr-1lo/CD11b+ and Gr-1hi/CD11b+ subsets defined immature and mature neutrophil populations, respectively.43 (link)
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