Fluorescence lifetime measurements were performed using Fluoro-Cube fluorescence lifetime system (Horiba Jobin Yvon) in nanosecond time domain equipped with a Nano LED (λex = 405 nm) source using 1 cm quartz cell at 25 °C. Fluorescence emission was detected at 470 nm by thermoelectrically cooled TBX-04-D detector. All decay traces were measured using 2048 channel analyzer. Typical parameters for these experiments were as follows: time resolution = 0.2 ns, wavelength accuracy = ±0.5 nm, speed = 150 nm/s, TAC range = 200 ns. The analysis of decay curves was based on multi-exponential iterative re-convolution technique using the DAS 6.3 software provided by IBH and the data were fitted for the best chi square value, while errors were obtained as standard deviation from the fit. The fluorescence lifetime measurements for coralyne were carried out in aqueous solution in the presence and absence of salt.
Fluoro cube fluorescence lifetime system
Manufactured by Horiba
The Fluoro-Cube fluorescence lifetime system is a specialized lab equipment designed for fluorescence lifetime measurements. It provides accurate and reliable data on the time-resolved fluorescence characteristics of samples.
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4 protocols using fluoro cube fluorescence lifetime system
1
Fluorescence Lifetime Analysis of Coralyne
2
Steady-state Spectroscopic Characterization of Compounds
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Steady-state absorption spectra were measured using a Lambda 900 (Perkin-Elmer) UV/vis/NIR spectrometer with a data interval of 0.5 nm. These spectra were taken with ca. 10–5 to 10–6 M solutions in quartz cells with a path length of 1 cm. Steady-state fluorescence spectra were measured using a FluoroMax-3 (JOBIN YVON, HORIBA) spectrofluorophotometer with a data interval of 1 nm. Time correlated single photon counting was recorded in a 1 cm quartz cell using a HORIBA Jobin Yvon FluoroCube Fluorescence Lifetime System equipped with NanoLEDs and LDs. A Hamamatsu (R3809 U) photomultiplier and a thermoelectrically cooled TBX-04-D detector were used to detect the emitted photons. These spectra were taken with ca. 10–6 M solutions in quartz cells with a path length of 1 cm. Solvents were degassed by bubbling with argon before use.
3
Photoluminescence Characterization of Solutions
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The solutions were characterized by measuring the steady-state ultraviolet-visible (UV-VIS) absorption/PL spectra and TR-PL. TR-PL was measured using a C11367-01 spectrometer (Hamamatsu Photonics) or a Fluorocube fluorescence lifetime system (HORIBA). The PLQY of the solutions was measured by an absolute PLQY measurement system (C11347-01, Hamamatsu Photonics), with an excitation wavelength of 337 nm. Before the TAS and TR-PL measurements, the solutions were deoxygenated with dry nitrogen gas to eliminate the deactivation of triplets. The effect of deoxygenation was confirmed by comparing τprompt and τTADF values with those reported by Uoyama et al. (5 (link)).
4
Spectroscopic and Electrochemical Characterization of Corroles
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UV–vis and fluorescence spectra
were recorded using a Cary 100 spectrometer and Hitachi F-4600 spectrofluorometer,
respectively. All 1H NMR measurements were performed using
a Bruker AVANCE 500 MHz spectrometer in CDCl3 and DMSO-d6.The ESI mass spectra were recorded using Bruker
Daltanics-microTOF in the positive ion mode in CH3CN. EPR
spectra were recorded using a Bruker X-band EPR instrument in DMSO.
Electrochemical measurements were carried out with a CHI 620E electrochemical
workstation. A three-electrode system was used that consisted of a
GC working electrode, an Ag/AgCl reference electrode, and a Pt-wire
counter electrode. The concentrations of all of the corroles used
were approximately 1 mM. UV–vis and fluorescence titrations
of1 with FeIII ions were carried out in DMSO.
Fluorescence lifetime measurements in the nanosecond time domain were
recorded in DMSO using a Horiba Jobin Yvon “fluorocube fluorescence
lifetime system” equipped with a NanoLED (635 nm) source. Theoretical
calculations were carried out using B3LYP functional and the 6-31G
basis set in the gas phase.
were recorded using a Cary 100 spectrometer and Hitachi F-4600 spectrofluorometer,
respectively. All 1H NMR measurements were performed using
a Bruker AVANCE 500 MHz spectrometer in CDCl3 and DMSO-d6.The ESI mass spectra were recorded using Bruker
Daltanics-microTOF in the positive ion mode in CH3CN. EPR
spectra were recorded using a Bruker X-band EPR instrument in DMSO.
Electrochemical measurements were carried out with a CHI 620E electrochemical
workstation. A three-electrode system was used that consisted of a
GC working electrode, an Ag/AgCl reference electrode, and a Pt-wire
counter electrode. The concentrations of all of the corroles used
were approximately 1 mM. UV–vis and fluorescence titrations
of
Fluorescence lifetime measurements in the nanosecond time domain were
recorded in DMSO using a Horiba Jobin Yvon “fluorocube fluorescence
lifetime system” equipped with a NanoLED (635 nm) source. Theoretical
calculations were carried out using B3LYP functional and the 6-31G
basis set in the gas phase.
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