Dynamics software 7

A DynaPro NanoStar (556-DPN, WYATT Technology, U.S.A.) was used for the dynamic light scattering (DLS) measurements. The laser was set at λ = 661 nm with the detector angle at 90° for measurements. Both EVs samples, EVs(208K) and EVs(SEC) were adjusted with PBS to give concentration ranges from 0.2 to 2.0 mg/mL. PBS buffer was filtered through a Millipore Millex-GV 0.22 µm PVDF filter. DLS spectra were acquired in a disposable cyclic olefin copolymer cuvette at 25 °C. Each spectrum was collected over 5 runs consisting of 10 ten-second scans. The five runs were then averaged. Dynamics software (7.5.0.17, WYATT Technology, U.S.A.) was used to acquire and analyze the spectra. The regularization method was used to fit the autocorrelation functions.

A DynaPro NanoStar (556-DPN, WYATT Technology, USA) was used for the dynamic light scattering (DLS) measurements. The laser was set at λ = 661 nm with the detector angle at 90° for measurements. The samples were adjusted with PBS to give concentration ranges from 0.2 mg/mL to 2.0 mg/mL. PBS buffer was filtered through a Millipore Millex-GV 0.22 μm PVDF filter. DLS spectra were acquired in a disposable cyclic olefin copolymer cuvette at 25 °C. Each spectrum was collected over 5 runs consisting of 10 ten-second scans. The 5 runs were then averaged. Dynamics software (7.5.0.17, WYATT Technology, USA) was used to acquire and analyze the spectra. The regularization method was used to fit the autocorrelation functions.