U lh75xeapo

All 12 samples were stained with a novel fluorescent near-infrared SSTR2-targeted peptide, MMC(FNIR-Tag)-TOC, developed by the Azhdarinia laboratory following their established protocol,^{9 (link)} and subsequently wet mounted using Fluoromount-G®. Samples were then imaged with an inverted Olympus IX71 microscope with a 20x objective (1-U2B825-U, Olympus), illuminated with a xenon light source (U-LH75XEAPO, Olympus), and and ORCA-Flash 4.0 digital CMOS camera (C11440-22CU, Hamamatsu Photonics K.K.). Images were collected as 2048 x 2048 pixel tiles at 16-bit depth using a manual stage with varying overlap.

Cultured neurons were incubated with ATPOS complex at a final ATPOS concentration of 600 nM in physiological salt solution (50 mM HEPES, 125 mM NaCl, 2.5 mM KCl, 2 mM CaCl₂, 1 mM MgCl₂, and 25 mM glucose; pH 7.4) containing 0.1% bovine serum albumin at room temperature. After 10 min incubation, the cells were washed four times with physiological salt solution containing 0.1% bovine serum albumin.
Fluorescence images were acquired with an inverted microscope (IX-71, Olympus) equipped with an EM-CCD camera (iXon EM+, Andor) and a 75 W xenon light source (U-LH75XEAPO, Olympus), using a × 40/0.95 NA dry objective lens (UPlanSApo, Olympus). Cy3 was excited at 520–550 nm and the emission was measured at wavelengths longer than 580 nm with a filter set (Olympus). Alexa488 was excited at 460–480 nm and the emission was measured at 495–540 nm with a filter set (Olympus). The extracellular solution was physiological salt solution containing 0.1% bovine serum albumin and 100 μM ARL 67156 (Sigma) at pH 7.4.

For Hematoxylin and eosin (H&E) staining, liver sections were paraffin-embedded, deparaffinized, rehydrated, and stained using standard protocols. Imaging was performed using an inverted microscope (Olympus, U-LH75XEAPO). Liver pathological scores were evaluated according to the scoring system adapted from the previous literature by two pathologists [13 (link)]. The pathological scoring ranges from zero to eleven, including biliary duct involvement (0–3), biliary epithelial proliferation (0–4), and mononuclear leucocytic infiltration (0–4).
Immunohistochemistry followed similar initial steps as H&E staining. After quenching endogenous peroxidase activity, sections were incubated with primary antibodies (CD4, CD8, CK-19, Rorγ) (Supplementary Fig. S4, Table. S2). overnight at 4 °C, followed by a 30-minute incubation with horseradish peroxidase-conjugated secondary antibodies at 37 °C. Post-washing with PBS, diaminobenzidine (DAB) was applied for visualization. Microscopic examination was conducted with a light microscope (Olympus).