Log In Sign up
The largest database of trusted experimental protocols

Em 910

Manufactured by Zeiss
Visit Supplier
Sourced in Germany, United States

The EM 910 is a transmission electron microscope (TEM) manufactured by Zeiss. It is designed to provide high-resolution imaging of samples at the nanometer scale. The EM 910 utilizes an electron beam to illuminate and magnify specimens, allowing for detailed analysis of their structure and composition.

Automatically generated - may contain errors

Lab products found in correlation

Dmp30, by Serva Electrophoresis (3 mentions) Glycidether, by Serva Electrophoresis (3 mentions) Nanosight lm10, by Malvern Panalytical (2 mentions) Zetasizer nano z, by Malvern Panalytical (2 mentions) Alhydrogel, by Brenntag (2 mentions) Zetasizer nano zs system, by Malvern Panalytical (1 mentions) Exo spin exosome purification kit, by Cell Guidance Systems (1 mentions) Zetaview instrument, by Particle Metrix (1 mentions) Em ice, by Leica camera (1 mentions) Talos l120c, by Thermo Fisher Scientific (1 mentions) Hyclone water, by GE Healthcare (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Trypsin, by Merck Group (1 mentions) Em 900, by Zeiss (1 mentions) Ja 14 rotor, by Beckman Coulter (1 mentions) Sw60ti rotor, by Beckman Coulter (1 mentions) Jem f200, by JEOL (1 mentions) Ultra 45 diamond knife, by Electron Microscopy Sciences (1 mentions) Em uc6 ultramicrotome, by Leica camera (1 mentions) Araldite, by Serva Electrophoresis (1 mentions)

48 protocols using em 910

1

Ultrastructural Analysis of Genz-Treated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lovo and HCT116 cells, cultured on aclar-fluoropolymer-sheets (Science Servoces, Munic, Germay) were treated as described with 1 μM or 10 μM Genz for 6 days. Cells were fixed in 2% formic aldehyde/2% glutaric aldehyde/1 mM CaCl2/1 mM MgCl2/100 mM cacodylate buffer pH 7.2, following post-fixation in 1% OsO4, Uranyl en bloc staining (1% uranyl-acetate in 75% ethanol), dehydration in ethanol and Epon-embedding (Serva, Heidelberg, Germany). Ultrathin sections were cut at 50 nm nominal thickness (UCT, Leica, Wetzlar, Germany), contrasted with uranyl and lead and observed with an EM910 (Carl Zeiss Oberkochen, Germany) equipped with a CCD-Camera (TRS, Morenweis, Germany).
Jennemann R., Volz M., Bestvater F., Schmidt C., Richter K., Kaden S., Müthing J., Gröne H.J, & Sandhoff R. (2021). Blockade of Glycosphingolipid Synthesis Inhibits Cell Cycle and Spheroid Growth of Colon Cancer Cells In Vitro and Experimental Colon Cancer Incidence In Vivo. International Journal of Molecular Sciences, 22(19), 10539.
+ Open protocol
+ Expand
2

Purification and Visualization of FCoV Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols
FCoV particles were purified and concentrated by ultracentrifugation using a sucrose cushion that contained 2% (wt/vol) paraformaldehyde to preserve virion morphology. Material from the resuspended pellets was mounted on a Pioloform (Plano, Wetzlar), carbon coated, glow discharged using 400μ copper-rhodium grids, washed with distilled water, and stained using 1% (wt/vol) uranyl acetate. The grids were examined with a Zeiss EM 910 transmission electron microscope at 80 kV. Images were taken using a magnification-calibrated side-mounted charge-coupled-device (CCD) camera (Megaview II; SIS, Muenster, Germany) at an instrumental magnification of ×80,000.
Ehmann R., Kristen-Burmann C., Bank-Wolf B., König M., Herden C., Hain T., Thiel H.J., Ziebuhr J, & Tekes G. (2018). Reverse Genetics for Type I Feline Coronavirus Field Isolate To Study the Molecular Pathogenesis of Feline Infectious Peritonitis. mBio, 9(4), e01422-18.
+ Open protocol
+ Expand
3

Visualizing ASO Nanoparticle Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols
ASO solutions were prepared in 0.9% saline at 400 μM concentration and analysed using TEM (EM910 by Zeiss, Germany). The samples were prepared by transferring a drop of ASO solution to a carbon-coated copper grid for 15 min and blotted dry, followed by staining for 3 min with 1% phosphotungstic acid. Samples were analysed at 120 kV.
Marchesi E., Cortesi R., Preti L., Rimessi P., Sguizzato M., Bovolenta M, & Perrone D. (2022). Antisense Oligonucleotides Conjugated with Lipophilic Compounds: Synthesis and In Vitro Evaluation of Exon Skipping in Duchenne Muscular Dystrophy. International Journal of Molecular Sciences, 23(8), 4270.
+ Open protocol
+ Expand
4

Nanoparticle Characterization and Drug Loading

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrodynamic diameters, polydispersity (PDI) and zeta potentials (ζ) of NPs were determined by Zetasizer Nano ZS system (Malvern Instruments Ltd., Malvern, UK). Transmission electron microscopy (TEM) images were recorded using Zeiss EM 910 for morphology analysis.
Drug loading efficiency was determined using HPLC. Briefly, 50 μL of NPs were dissolved in 200 μL acetonitrile, vortexed for 1 min, and sonicated for 10 min. The obtained solution (10 μL) was analyzed by HPLC and drug load was calculated using a standard concentration curve. All measurements were determined in triplicate.
Yang F., Medik Y., Li L., Tian X., Fu D., Brouwer K.L., Wagner K., Sun B., Sendi H., Mi Y, & Wang A.Z. (2020). Nanoparticle drug delivery can reduce the hepatotoxicity of therapeutic cargo. Small (Weinheim an der Bergstrasse, Germany), 16(7), e1906360.
+ Open protocol
+ Expand
5

Electron Microscopy of Viral Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols
VLP and PsV preparations or tissue were fixed with buffered aldehyde solution (2% formaldehyde, 2% glutaraldehyde, 1 mM MgCl2, 2% sucrose in 100 mM calcium cacodylate, pH7.2), followed by post-fixation in buffered 1% OsO4, graded dehydration with ethanol and resin-embedding in epoxide (12 g glycid ether, 6.5 g NMA, 6.5 g DDSA, 400 μl DMP30, all from Serva, Germany). Ultrathin sections at nominal thickness 60 nm and contrast-stained with lead-citrate and Uranylacetate were observed in a Zeiss EM 910 at 100 kV (Carl Zeiss, Oberkochen, Germany) and micrographs were taken with image-plates, scanned at 30 µm resolution (Ditabis micron, Pforzheim, Germany).
Fu Y., Cao R., Schäfer M., Stephan S., Braspenning-Wesch I., Schmitt L., Bischoff R., Müller M., Schäfer K., Vinzón S.E., Rösl F, & Hasche D. (2020). Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity. eLife, 9, e57626.
+ Open protocol
+ Expand
6

Visualizing GFP Expression Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
N or the indicated N mutants at a final concentration of 1.5 mg/ml were mixed with an in vitro-transcribed 8-kb single-stranded RNA comprising a Green Fluorescent Protein (GFP) expression construct (27 (link)) at a final concentration of 0.15 mg/ml. The sample was 10-fold diluted with 20-mM TRIS, pH 7.5, 150-mM NaCl, and 15 μl of this solution was spotted onto carbon-coated grids. The sample was stained with 2% uranyl acetate and images were acquired on a Zeiss EM910 at magnifications between 12.5 and 31.5 k.
Olal D., Dick A., Woods VL J.r., Liu T., Li S., Devignot S., Weber F., Saphire E.O, & Daumke O. (2014). Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein. Nucleic Acids Research, 42(9), 6025-6037.
+ Open protocol
+ Expand
7

Ultrastructural Analysis of L1 Isoforms

Check if the same lab product or an alternative is used in the 5 most similar protocols
L1 isoform preparations were fixed with buffered aldehyde solution (2% formaldehyde, 2% glutaraldehyde, 1 mM MgCl2, 2% sucrose in 100 mM calcium cacodylate, pH7.2), followed by post-fixation in buffered 1% OsO4, graded dehydration with ethanol and resin-embedding in epoxide (12 g glycid ether, 6.5 g NMA, 6.5 g DDSA, 400 μl DMP30; all from Serva, Heidelberg, Germany). Ultrathin sections at nominal thickness 60 nm and contrast-stained with lead-citrate and Uranylacetate were observed in a Zeiss EM 910 at 100 kV (Carl Zeiss, Oberkochen, Germany) and micrographs were taken with image-plates, scanned at 30 µm resolution (Ditabis micron, Pforzheim, Germany).
Ahmels M., Mariz F.C., Braspenning-Wesch I., Stephan S., Huber B., Schmidt G., Cao R., Müller M., Kirnbauer R., Rösl F, & Hasche D. (2022). Next generation L2-based HPV vaccines cross-protect against cutaneous papillomavirus infection and tumor development. Frontiers in Immunology, 13, 1010790.
+ Open protocol
+ Expand
8

Negative Staining of Mgm1 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
For electron microscopy of negatively stained samples in a Zeiss EM910, 4 μM Mgm1 (amino acids 219-912) in 25 mM HEPES-NaOH (pH 7.8), 60 mM NaCl, 100 mM KCl, 0.5 mM MgC12 and 3 mM guanosine-5'-[(β,γ)-methyleno]triphosphate were incubated at room temperature for 10 min. The final concentration of unfiltered Folch liposomes was 0.6 mg/ml. Samples were incubated on carbon-coated copper grids (Plano GmbH, Wetzlar, Germany) and stained with 2% uranyl acetate.
Faelber K., Dietrich L., Noel J.K., Wollweber F., Pfitzner A.K., Mühleip A., Sánchez R., Kudryashev M., Chiaruttini N., Lilie H., Schlegel J., Rosenbaum E., Hessenberger M., Matthaeus C., Kunz S., von der Malsburg A., Noé F., Roux A., van der Laan M., Kühlbrandt W, & Daumke O. (2019). Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1. Nature, 571(7765), 429-433.
+ Open protocol
+ Expand
9

Characterization of SiNP Size and Charge

Check if the same lab product or an alternative is used in the 5 most similar protocols
The hydrodynamic size and zeta potential of SiNP were analyzed at room temperature using a Malvern Zetasizer, Nano ZSP, equipped with a standard 633 nm laser. Transmission electron microscopy (TEM, EM910, Carl Zeiss) was used to measure the size of the nanoparticles.
Sheshachala S., Grösche M., Scherr T., Hu Y., Sun P., Bartschat A., Mikut R, & Niemeyer C.M. (2020). Segregation of Dispersed Silica Nanoparticles in Microfluidic Water‐in‐Oil Droplets: A Kinetic Study. Chemphyschem, 21(10), 1070-1078.
+ Open protocol
+ Expand
10

Transmission Electron Microscopy of Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bacteria were fixed with buffered aldehyde solution (4% formaldehyde, 2% glutaraldehyde, 1 mM MgCl2, 1 mM CaCl2 in 100 mM calcium cacodylate, pH7.1), followed by post-fixation in buffered 1% OsO4, graded dehydration with ethanol and resin-embedding in epoxide (12 g glycid ether, 6.5 g N,N-dimethylacetamide (NMA), 6.5 g dodecenylsuccinic anhydride (DDSA), 400 mL 2,4,6-Tris(dimethylaminomethyl)phenol (DMP30), all from Serva, Heidelberg, Germany). Ultrathin sections at nominal thickness 60 nm and contrast-stained with lead-citrate and uranyl acetate were observed in a Zeiss EM 910 at 100 kV (Carl Zeiss, Oberkochen, Germany).
Nurjadi D., Boutin S., Schmidt K., Ahmels M, & Hasche D. (2020). Identification and Elimination of the Clinically Relevant Multi-Resistant Environmental Bacteria Ralstonia insidiosa in Primary Cell Culture. Microorganisms, 8(10), 1599.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!