Anti phosphorylated iκb α

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-phosphorylated (p)-IκB-α is a laboratory product used to detect and quantify the phosphorylated form of the IκB-α protein. IκB-α is a regulatory protein that controls the activity of the transcription factor NF-κB, which plays a crucial role in inflammatory and immune responses. The anti-p-IκB-α product can be used in various techniques, such as Western blotting and ELISA, to measure the levels of phosphorylated IκB-α in biological samples.

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Lab products found in correlation

2 protocols using anti phosphorylated iκb α

Anti-inflammatory and Antioxidant Effects

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GC (PubChem CID: 161120) and PQ were obtained from Sigma-Aldrich (Sigma, MO, USA). Anti-Nrf2, anti-HO-1, anti-NQO-1, anti-GCLM, anti-ICAM-1, anti-VCAM-1, anti-iNOS, anti-IKK-β, anti-IκB-α, anti-NF-κB p65, anti-phosphorylated (p)-IκB-α, anti-Bcl-2, anti-Bcl-xl, anti-Bax, anti-Caspase-3, anti-Caspase-9, anti-GAPDH, anti-β-actin, anti-histone, and IgG-HRP antibodies were products of Santa Cruz Biotechnology (Santa Cruz, Texas, USA). BCA protein concentration assay kit, PVDF membranes, and SDS-PAGE gel preparation kit were purchased from Beyotime Institute of Biotechnology. ECL plus kit was obtained from Nanjing KeyGen Biotech Co., Ltd. (KeyGen, Nanjing, CN). TNF-α, IL-1β, and IL-6 ELISA kits were obtained from Abcam (Cambrige, UK). GSH, NADPH, SOD, CAT, MDA, CK, and LDH kits were products of Nanjing Jiancheng Engineering Institute (Nanjing, CN).

Zhang R., Zhao C., Gong X., Yang J., Zhang G, & Zhang W. (2022). Ginkgolide C Alleviates Acute Lung Injury Caused by Paraquat Poisoning via Regulating the Nrf2 and NF-κB Signaling Pathways. Oxidative Medicine and Cellular Longevity, 2022, 7832983.

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Inflammatory Modulation by GC and LPS

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GC (PubChem CID: 161120) and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Merck KGaA). TNF-α, IL-1β, IL-6 ELISA kits and anti-IKK-β antibody were purchased from Abcam. Anti-CD40, anti-ICAM-1, anti-VCAM-1, anti-iNOS, anti-NF-κB p65, anti-phosphorylated (p)-IκB-α, anti-IκB-α, anti-β-actin, anti-histone and goat anti-mouse IgG-HRP antibodies were purchased from Santa Cruz Biotechnology, Inc. Phenylmethanesulfonyl fluoride and RIPA lysis buffer were purchased from Abcam. PVDF membranes, the BCA protein concentration assay kit and the SDS-PAGE gel preparation kit were obtained from Beyotime Institute of Biotechnology. The ECL plus kit was purchased from Cusabio Technology LLC.

Zhang R., Guo N., Yan G., Wang Q., Gao T., Zhang B, & Hou N. (2021). Ginkgolide C attenuates lipopolysaccharide-induced acute lung injury by inhibiting inflammation via regulating the CD40/NF-κB signaling pathway. International Journal of Molecular Medicine, 47(4), 62.

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