Ncrnu athymic mice

Further, 6–8 weeks female NCRNU athymic mice were purchased from Taconic Biosciences. All animals were housed in the American Association for Laboratory Animal Care–accredited Animal Resource Center at Moffitt Cancer Center. All animal procedures and experiments were carried out under protocols approved by the Institutional Animal Care and Use Committee of the University of South Florida and Moffitt Cancer Center. All animal studies were performed in accordance with relevant guidelines and regulations of University of South Florida and Moffitt Cancer Center. For orthotopic model, xenograft tumors were established by intracranially injecting 1 × 105 indicated GSCs in a 2–3 μL volume of PBS in the right striatum of mice (n = 5/group) on a Stoelting Digital Stereotaxic Instrument (Stoelting, Wood Dale, IL, USA). Seven days after implantation, the mice were randomized into distinct groups for treatment (as indicated in figures). TMZ, 50 mg/kg/day; 5-Aza, 5 mg/kg/day; Lomeguatrib, 20 mg/kg/day. For survival studies, animals were followed every day until they lost 20% of body weight or had trouble ambulating, feeding or grooming.

6–8 weeks female NCRNU athymic mice were obtained from Taconic Biosciences. All animals were housed in the AALAC–accredited Animal Resource Center of the Moffitt Cancer Center. All animal studies were performed under protocols approved by the Institutional Animal Care and Use Committee.

6–8 weeks female NCRNU athymic mice were ordered from Taconic Biosciences. All animals were housed in the American Association for Laboratory Animal Care–accredited Animal Resource Center at Moffitt Cancer Center. All animal procedures and Experiments were carried out under protocols approved by the Institutional Animal Care and Use Committee of the University of South Florida and Moffitt Cancer Center. All animal studies were performed in accordance with relevant guidelines and regulations of University of South Florida and Moffitt Cancer Center.