The substrate (10 mM pyruvate), 4 mM phosphate (KH2PO4), 1 U of Amplex Ultra Red reagent, 4 U of horseradish peroxidase (Amresco, Solon, OH, USA), and 0.2 mg of mitochondria were added to 1 mL of isolation buffer (225 mM mannitol, 75 mM sucrose, 5 mM Hepes (pH 7.4), 1 mM EGTA, 2 mg/mL fatty acids free BSA. The H2O2 concentration was measured as the fluorescence intensity of resorufin formed during the reaction upon oxidation of Amplex Ultra Red. Production changes were recorded after the addition of 0.2 mg cisplatin, 1 μM methylene blue, and 1 μM azure B.
Horseradish peroxidase
Horseradish peroxidase is an enzyme commonly used in laboratory settings. It catalyzes the oxidation of various substrates in the presence of hydrogen peroxide.
Lab products found in correlation
5 protocols using horseradish peroxidase
Mitochondrial H2O2 Production Assay
The substrate (10 mM pyruvate), 4 mM phosphate (KH2PO4), 1 U of Amplex Ultra Red reagent, 4 U of horseradish peroxidase (Amresco, Solon, OH, USA), and 0.2 mg of mitochondria were added to 1 mL of isolation buffer (225 mM mannitol, 75 mM sucrose, 5 mM Hepes (pH 7.4), 1 mM EGTA, 2 mg/mL fatty acids free BSA. The H2O2 concentration was measured as the fluorescence intensity of resorufin formed during the reaction upon oxidation of Amplex Ultra Red. Production changes were recorded after the addition of 0.2 mg cisplatin, 1 μM methylene blue, and 1 μM azure B.
Monoclonal Antibody Production and Purification
Selected mAbs were conjugated with horse-radish peroxidase (Amresco, USA) using optimized NaIO4 method as described previously [36 (link)].
Phospholipase D Activity Assay
Phospholipase D2 Activity Assay
Lipid Substrate Assay for Phospholipase D
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