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Rabbit anti gapdh monoclonal antibody

Manufactured by Merck Group
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Rabbit-anti-GAPDH monoclonal antibody is a laboratory reagent used for the detection and quantification of the GAPDH protein in biological samples. GAPDH, or Glyceraldehyde 3-phosphate dehydrogenase, is a widely used reference protein for various analytical techniques.

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Lab products found in correlation

Rabbit anti hif 1α monoclonal antibody, by Merck Group (3 mentions) Rabbit anti vegf monoclonal antibody, by Merck Group (3 mentions) Diazoxide, by Merck Group (2 mentions) Ros scavenger n 2 mercaptopropionylglycine, by Merck Group (2 mentions) Hif 1 agonist dimethyloxaloylglycine, by Merck Group (2 mentions) Anti sfrp1, by Abcam (2 mentions) Odyssey imaging system, by LI COR (1 mentions) Gapdh, by Merck Group (1 mentions) Anti axin2, by Abcam (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti dkk3, by GeneTex (1 mentions) Nuclear extraction kit, by Active Motif (1 mentions) Anti hdac1, by Santa Cruz Biotechnology (1 mentions) Anti β catenin, by Merck Group (1 mentions)

6 protocols using rabbit anti gapdh monoclonal antibody

1

Cardioprotective Preconditioning Mechanisms

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Diazoxide, mitoKATP blocker −5-hydroxydecanoate (5-HD), ROS scavenger—N-2-mercaptopropionylglycine (MPG), and HIF-1 agonist—dimethyloxaloylglycine (DMOG) were purchased from Sigma (St. Louis, MO, USA). Rabbit-anti-HIF-1α monoclonal antibody, rabbit-anti VEGF monoclonal antibody, and rabbit-anti-GAPDH monoclonal antibody were purchased from Sigma. Pentobarbital was purchased from Shanghai Tyrael Biological Technology Co., Ltd. The study was approved by the Institutional Review Board of The Affiliated Hospital of Zunyi Medical University, Guizhou, China.
Chen X.Y., Wang J.Q., Cheng S.J., Wang Y., Deng M.Y., Yu T., Wang H.Y, & Zhou W.J. (2021). Diazoxide Post-conditioning Activates the HIF-1/HRE Pathway to Induce Myocardial Protection in Hypoxic/Reoxygenated Cardiomyocytes. Frontiers in Cardiovascular Medicine, 8, 711465.
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2

Investigating Hypoxia Signaling Pathways

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Sevoflurane was purchased from Maruishi Pharmaceutical, Japan. Rabbit-anti-HIF-1α monoclonal antibody, rabbit-anti-VEGF monoclonal antibody, rabbit-anti-eNOS monoclonal antibody, and rabbit-anti-GAPDH monoclonal antibody were purchased from Sigma, USA. HIF-1α inhibitor 2ME2 was purchased from selleck, USA. Pentobarbital was purchased from Shanghai Tyrael Biological Technology Co., LTD.
Wu J., Yang L., Xie P., Yu J., Yu T., Wang H., Maimaitili Y., Wang J., Ma H., Yang Y, & Zheng H. (2017). Cobalt Chloride Upregulates Impaired HIF-1α Expression to Restore Sevoflurane Post-conditioning-Dependent Myocardial Protection in Diabetic Rats. Frontiers in Physiology, 8, 395.
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3

Cardioprotective Preconditioning Mechanisms

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Diazoxide, mitoKATP blocker −5-hydroxydecanoate (5-HD), ROS scavenger—N-2-mercaptopropionylglycine (MPG), and HIF-1 agonist—dimethyloxaloylglycine (DMOG) were purchased from Sigma (St. Louis, MO, USA). Rabbit-anti-HIF-1α monoclonal antibody, rabbit-anti VEGF monoclonal antibody, and rabbit-anti-GAPDH monoclonal antibody were purchased from Sigma. Pentobarbital was purchased from Shanghai Tyrael Biological Technology Co., Ltd. The study was approved by the Institutional Review Board of The Affiliated Hospital of Zunyi Medical University, Guizhou, China.
Chen X.Y., Wang J.Q., Cheng S.J., Wang Y., Deng M.Y., Yu T., Wang H.Y, & Zhou W.J. (2021). Diazoxide Post-conditioning Activates the HIF-1/HRE Pathway to Induce Myocardial Protection in Hypoxic/Reoxygenated Cardiomyocytes. Frontiers in Cardiovascular Medicine, 8, 711465.
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4

Angpt1 Western Blot Protocol

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Western blot analysis of Angpt1 was performed with 50 μg of protein extract using polyclonal antibodies to Angpt1 (1:100; Abcam, Cambridge, United Kingdom), VWF (1:1,000; ABclonal, China), and the appropriate IRDyeTM 800‐conjugated secondary antibody (1:10,000). Signals were detected using the Odyssey Imaging System (LI‐COR Biosciences, Lincoln, NE) and analyzed with Odyssey software. Results were normalized relative to the glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (rabbit anti‐GAPDH monoclonal antibody, 1:1,000; Sigma‐Aldrich, St. Louis, MO) expression to correct for variations in protein loading and transfer.
Yang L., Yue W., Zhang H., Zhang Z., Xue R., Dong C., Liu F., Chang N., Yang L, & Li L. (2022). Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis. Hepatology Communications, 6(6), 1425-1442.
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5

Western Blot Analysis of Wnt Pathway Proteins

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Western blot analysis was performed according to a previously described standard method51 (link) using anti-AXIN2 (1:500; Abcam, Cambridge, MA), anti-DKK3 (1:500; GeneTex, San Antonio, TX), anti-SFRP1 (1:500; Abcam) or anti-β-catenin (1:1,000; Cell Signaling, Danvers, MA) antibodies. Blotted membranes were stripped and re-blotted with anti-GAPDH rabbit monoclonal antibody (1:2000; Sigma, St. Louis, MO) as a loading control. Original data of immunoblotting are presented in Supplementary Fig. 8.
Fang L., Cai J., Chen B., Wu S., Li R., Xu X., Yang Y., Guan H., Zhu X., Zhang L., Yuan J., Wu J, & Li M. (2015). Aberrantly expressed miR-582-3p maintains lung cancer stem cell-like traits by activating Wnt/β-catenin signalling. Nature Communications, 6, 8640.
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6

Western Blot and Nuclear Extraction Analysis

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For western blots, total cellular protein was extracted from cells and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Nuclear extracts were prepared using the Nuclear Extraction Kit (ActiveMotif), according to the manufacturer's instructions. The following antibodies were used: anti-β-catenin (Millipore, Billerica, MA), anti-LZTFL1 (Sigma, St. Louis, MO), anti-SFRP1 (Abcam), anti-DKK2 (GeneTex, San Antonio, TX), anti-HDAC1 (Santa Cruz Biotechnology, Santa Cruz, CA), anti-GAPDH rabbit monoclonal antibody (Sigma, St. Louis, MO).
Lu J., Wei J.H., Feng Z.H., Chen Z.H., Wang Y.Q., Huang Y., Fang Y., Liang Y.P., Cen J.J., Pan Y.H., Liao B., Chen W.F., Chen W, & Luo J.H. (2017). miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/β-catenin signalling. Oncotarget, 8(13), 21461-21471.
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