Anti ha

Manufactured by Abbkine

Anti-HA is a laboratory reagent used for the detection and analysis of proteins tagged with the hemagglutinin (HA) epitope. It provides a specific and efficient tool for immunoaffinity purification, immunoprecipitation, and Western blotting applications.

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Lab products found in correlation

Np 40 lysis buffer, by Beyotime (1 mentions) Anti nav1, by Alomone (1 mentions) Protease inhibitor mixture cocktail, by Roche (1 mentions) Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Anti ankg, by Santa Cruz Biotechnology (1 mentions) Quinidine, by Maclin Biochemical Technology (1 mentions) Anti flag, by Abbkine (1 mentions) A02010, by Abbkine (1 mentions)

2 protocols using anti ha

Investigating Sodium Channel Interactome

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Commercial antibodies used were anti-AnkG (Santa Cruz), anti-Slack (NeuroMab), anti-Na_V1.2 (Alomone), anti-Na_V1.6 (Alomone), anti-HA (Abbkine), anti-Flag (Abbkine), anti-β-actin (Biodragon), HRP goat anti-mouse IgG LCS (Abbkine), HRP mouse anti-rabbit IgG LCS (Abbkine), Alexa Fluor 488-AffinityPure Fab Fragment donkey anti-rabbit IgG (Jackson), and Alexa Fluor 594 donkey anti-mouse IgG (Yeason). GPCR Extraction Reagent was from Pierce, NP40 lysis buffer was from Beyotime, protease inhibitor mixture cocktail was from Roche Applied Science, rabbit IgG and mouse IgG were from Santa Cruz, and Protein G Dynabeads were from Invitrogen. Tetrodotoxin was from Absin Bioscience. Quinidine was from Macklin, and riluzole was from Meilunbio. All other reagents were purchased from Sigma-Aldrich.

Yuan T., Wang Y., Jin Y., Yang H., Xu S., Zhang H., Chen Q., Li N., Ma X., Song H., Peng C., Geng Z., Dong J., Duan G., Sun Q., Yang Y., Yang F, & Huang Z. (2024). Coupling of Slack and NaV1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development. eLife, 12, RP87559.

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Co-Immunoprecipitation of VAMP2 and APOE

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For Co-IP analysis, HEK-293T cells were co-transfected with pCMV-VAMP2-HA and pCMV-APOE3-3 × Flag or pCMV-APOE4 × 3Flag plasmids. After 48 h of transfection, the cells were washed with PBS and lysed with IP lysis buffer for 30 min at 4°C. The supernatant of cell lysates was incubated with the respective antibodies overnight at 4°C, followed by treatment with protein A/G PLUS-Agarose for 2 h at 4°C. Beads were washed three times with lysis buffer before suspension in an equal volume of 2× SDS sample buffer. Protein bands were detected by western blotting using the anti-Flag (Abbkine, A02010) and anti-HA (Abbkine, A02040) antibodies.

Chen F., Chen Y., Chen H., Wang Y., Wei W., Cai Y., Liang C., Cao H., Chen X., Yao J., Zhang D., Sun Y., Wang Y., & Cui L. (2021). APOE4 Regulates Synaptic Function by Directly Modulating the SNARE Complex Assembly.

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